Effects of combined application of phosphorus and zinc on growth and physiological characteristics of apple rootstock M9-T337 seedlings (Malus domestica Borkh.)

Abstract

Background Balancing nutrient application is crucial for plant growth. However, excessive fertilizer use, especially imbalanced applications of macronutrients such as phosphate (P), can hinder plant uptake of micronutrients. Balanced P and zinc (Zn) are vital for apple yield and quality, and apple trees are highly sensitive to deficiencies in these nutrient s . Therefore, this study was conducted in May 2022, employed a sand culture experiment to investigate the effects of varying P and Zn levels on the growth phenotype, photosynthetic capacity, antioxidant enzyme activity, sugar composition, endogenous hormone levels, and nutrient absorption and utilization of M9-T337 seedlings. Three levels of P (low, medium, high) and three levels of Zn (low, medium, high) were combined to create a total of nine distinct treatment. Results The results indicate that combined P and Zn fertilization at various levels exerts either synergistic or antagonistic effects on the growth, nutrient absorption, and utilization of M9-T337 seedlings. Compared to low and medium levels of P, a combination of high P (4 mmol·L −1 ) and an adequate amount of Zn significantly enhanced plant growth, root system development, and the microstructure of leaves. Notably, seedlings treated with high P and low Zn (HPLZn) reached a height 1.54 times that of the medium P and medium Zn (MPMZn, control). Physiological indicators under HP conditions revealed significant increases in antioxidant enzyme activity, leaf water retention, photosynthetic pigment concentration, osmotic adjustment substances, and the contents of glucose, sucrose, fructose, endogenous hormones, as well as P and Zn accumulation in the leaves, compared to the control. However, an increase in Zn application led to a declining trend in these parameters. Specifically, the HPLZn treatment exhibited substantial increases in Net photosynthetic rate ( Pn ), Total chlorophyll (Chl a + b), glucose, fructose, sucrose, and Auxin(IAA), with increments of 7.12%, 27.32%, 11.40%, 23.20%, 16.67%, and 55.11%, respectively, compared to the control. Conclusion Based on the comprehensive ranking from principal component analysis, the combination of HP ( 4 mmol·L −1 ) and LZn (0.5 µmol·L −1 ) was found to be the most effective in enhancing the antioxidant capacity, sugar accumulation, osmotic regulation ability, photosynthetic capacity, endogenous hormone levels, as well as P and Zn nutrient absorption and utilization in M9-T337 seedlings. Supplementary Information The online version contains supplementary material available at 10.1186/s12870-024-05724-y.