Effects of combined radiofrequency radiation exposure on the cell cycle and its regulatory proteins

Lee, Kwan-Yong; Kim, Bong Cho; Han, Na-Kyung; Lee, Yun‐Sil; Kim, Taejoon; Yun, Jae-Hoon; Kim, Nam; Pack, Jeong‐Ki; Lee, Jae‐Seon

doi:10.1002/bem.20618

Cited by 21 publications

(11 citation statements)

References 79 publications

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“…Even though a considerable number of epidemiological, in vivo, and in vitro studies have been conducted to provide scientific evidences for health risk assessment [Rothman, 2000; Meltz, 2003; Verschaeve et al, 2010], no reports regarding biological effects of exposure to multiple RF radiation signals are available except our group's recent reports. We recently demonstrated that combinations of CDMA signals at 837 MHz and W‐CDMA signals at 1950 MHz did not affect cell cycle progression in vitro [Lee et al, 2011b]. We also reported that combined exposure to CDMA and W‐CDMA RF radiation did not cause any observable adverse effects on mouse fetuses [Lee et al, 2009].…”

Section: Discussionmentioning

confidence: 95%

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Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or combined on oxidative stress in MCF10A cells

Hong

Kim

et al. 2012

Bioelectromagnetics

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The aim of this study was to determine whether the exposure to either single or multiple radio-frequency (RF) radiation frequencies could induce oxidative stress in cell cultures. Exposures of human MCF10A mammary epithelial cells to either a single frequency (837 MHz alone or 1950 MHz alone) or multiple frequencies (837 and 1950 MHz) were conducted at specific absorption rate (SAR) values of 4 W/kg for 2 h. During the exposure period, the temperature in the exposure chamber was maintained isothermally. Intracellular levels of reactive oxygen species (ROS), the antioxidant enzyme activity of superoxide dismutase (SOD), and the ratio of reduced/oxidized glutathione (GSH/GSSG) showed no statistically significant alterations as the result of either single or multiple RF radiation exposures. In contrast, ionizing radiation-exposed cells, used as a positive control, showed evident changes in all measured biological endpoints. These results indicate that single or multiple RF radiation exposure did not elicit oxidative stress in MCF10A cells under our exposure conditions.

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Section: Discussionmentioning

confidence: 95%

“…A radial transmission line (RTL) exposure system was used as an in vitro multi‐frequency radiation exposure system for this study [Lee et al, 2011b]. A typical CDMA signal at 837 MHz and a W‐CDMA signal at 1950 MHz were applied to the RTL after amplification.…”

Section: Methodsmentioning

confidence: 99%

Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or combined on oxidative stress in MCF10A cells

Hong

Kim

et al. 2012

Bioelectromagnetics

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“…We reported that combined exposure of CDMA and WCDMA RF radiation did not cause any observable adverse effects on mouse fetuses [24]. We demonstrated that combinations of CDMA signal at 837 MHz and WCDMA signal at 1950 MHz did not affect cell cycle progression in vitro [3]. We also observed no effect of combined RF radiation on ROS level, superoxide dismutase activity, or ratio of reduced/oxidized glutathione in human mammary epithelial MCF10A cells at 10, 24 or 48 h after exposure [25].…”

Section: Discussionmentioning

confidence: 99%

“…The details about this exposure system have been described previously [3]. A typical CDMA signal of 837 MHz and a WCDMA signal of 1950 MHz were applied to the RTL after amplification.…”

Section: Methodsmentioning

confidence: 99%

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Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells

Kang

Lee

et al. 2013

Journal of Radiation Research

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The objective of this study was to investigate the effects of the combined RF radiation (837 MHz CDMA plus 1950 MHz WCDMA) signal on levels of intracellular reactive oxygen species (ROS) in neuronal cells. Exposure of the combined RF signal was conducted at specific absorption rate values of 2 W/kg of CDMA plus 2 W/kg of WCDMA for 2 h. Co-exposure to combined RF radiation with either H2O2 or menadione was also performed. The experimental exposure groups were incubator control, sham-exposed, combined RF radiation-exposed with or without either H2O2 or menadione groups. The intracellular ROS level was measured by flow cytometry using the fluorescent probe dichlorofluorescein diacetate. Intracellular ROS levels were not consistently affected by combined RF radiation exposure alone in a time-dependent manner in U87, PC12 or SH-SY5Y cells. In neuronal cells exposed to combined RF radiation with either H2O2 or menadione, intracellular ROS levels showed no statically significant alteration compared with exposure to menadione or H2O2 alone. These findings indicate that neither combined RF radiation alone nor combined RF radiation with menadione or H2O2 influences the intracellular ROS level in neuronal cells such as U87, PC12 or SH-SY5Y.

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Study of p53 expression and post‐transcriptional modifications after GSM‐900 radiofrequency exposure of human amniotic cells

Bourthoumieu

Magnaudeix

Terro

et al. 2012

Bioelectromagnetics

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The potential effects of radiofrequency (RF) exposure on the genetic material of cells are very important to determine since genome instability of somatic cells may be linked to cancer development. In response to genetic damage, the p53 protein is activated and can induce cell cycle arrest allowing more time for DNA repair or elimination of damaged cells through apoptosis. The objective of this study was to investigate whether the exposure to RF electromagnetic fields, similar to those emitted by mobile phones of the second generation standard, Global System for Mobile Communications (GSM), may induce expression of the p53 protein and its activation by post-translational modifications in cultured human cells. The potential induction of p53 expression and activation by GSM-900 was investigated after in vitro exposure of human amniotic cells for 24 h to average specific absorption rates (SARs) of 0.25, 1, 2, and 4 W/kg in the temperature range of 36.3-39.7 °C. The exposures were carried out using a wire-patch cell (WPC) under strictly controlled conditions of temperature. Expression and activation of p53 by phosphorylation at serine 15 and 37 were studied using Western blot assay immediately after three independent exposures of cell cultures provided from three different donors. Bleomycin-exposed cells were used as a positive control. According to our results, no significant changes in the expression and activation of the p53 protein by phosphorylation at serine 15 and 37 were found following exposure to GSM-900 for 24 h at average SARs up to 4 W/kg in human embryonic cells.

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Effects of combined radiofrequency radiation exposure on the cell cycle and its regulatory proteins

Cited by 21 publications

References 79 publications

Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or combined on oxidative stress in MCF10A cells

Effects of 837 and 1950 MHz radiofrequency radiation exposure alone or combined on oxidative stress in MCF10A cells

Effects of combined radiofrequency radiation exposure on levels of reactive oxygen species in neuronal cells

Study of p53 expression and post‐transcriptional modifications after GSM‐900 radiofrequency exposure of human amniotic cells

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