Effects of Commercial Probiotics on Colonic Sensitivity after Acute Mucosal Irritation

Abstract

Gastrointestinal pathologies associated with abdominal pain, such as irritable bowel syndrome or inflammatory bowel disease, lack sufficiently effective treatments. In our study we have used a rat model of visceral pain (72 animals; n = 8–13 per experimental group) to analyze the consequences of intracolonic administration of the irritant acetic acid on visceral sensitivity, histology of the colonic wall, and inflammatory response. Moreover, we have studied the possible beneficial effects of a pretreatment with a commercial probiotic (Actimel®). Contrary to expectations, acetic acid application (7 cm proximal to the anus) decreased the nociceptive response to intracolonic mechanical stimulation, with a slight increase in the histological damage of colonic mucosa. The intensity of these changes depended on the concentration (4% or 0.6%) and the time of application (30 or 60 min). Pretreatment with probiotics (by daily gavage, for 1 week) normalized the values obtained in the visceral sensitivity test but revealed an increase in the number of macrophages. These results suggest a possible activation of inhibitory mechanisms early after colonic irritation, not previously described (which need further experimental confirmation), and the ability of probiotics to normalize the effects of acetic acid. In addition, pretreatment with probiotics has a direct effect on immune functions, stimulating macrophagic activity.

Effects of vincristine and monosodium glutamate on gastrointestinal motility and visceral sensitivity

“…Colorectal sensitivity was measured in cohort 2 as previously described. 16,25,26 Briefly, after sedation with Sedator® (medetomidine hydrochloride, 1 mg kg −1 , ip), a 10 cm longitudinal line was drawn over the linea alba of the abdomen. Transverse lines were drawn every 2 cm to better visualize the contractions during the recordings.…”