Effects of composite antimicrobial peptide on growth performance and health in weaned piglets

Shi, Jiankai; Zhang, Pan; Xu, Meng; Fang, Zhengfeng; Lin, Yan; Che, Lianqiang; Feng, Bin; Li, Jian; Li, Gang; Wu, De; Xu, Shengyu

doi:10.1111/asj.12933

Cited by 12 publications

(9 citation statements)

References 30 publications

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“…As reported, AMP P5 can increase the apparent digestibility of total dry matter, crude protein and total energy of weaned piglets (Yoon et al, 2013). Compound antimicrobial peptide (CAP) can increase the total apparent digestibility of dry matter and crude ash of weaned piglets (Shi et al, 2018). The AMP KR-32 can improve the apparent digestibility of crude protein and crude fat in piglets infected with E. coli (Liu et al, 2019).…”

Section: Discussionmentioning

confidence: 93%

APB‐13 improves the adverse outcomes caused by TGEV infection by correcting the intestinal microbial disorders in piglets

Liang

Wang

Lian

et al. 2021

Animal Physiology Nutrition

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Porcine transmissible gastroenteritis virus (TGEV) is an enteric coronavirus that has caused high morbidity and mortality of piglets worldwide. Previous studies have shown that the TGEV can lead to severe diarrhoea, vomiting and dehydration in 2-week-old piglets and weaned piglets, resulting in a large number of piglet deaths.Antimicrobial peptides have broad-spectrum antimicrobial activity and a strong killing effect on bacteria, especially on the drug-resistant pathogenic bacteria, and it has attracted broad concern. However, there are very few reports on the effect of APB-13 (an antimicrobial peptide) on the intestinal microbes of piglets infected with TGEV. In this study, 16S rRNA gene sequencing was used to compare the microbial phylum and the genus of piglet's enteric microorganism in different experimental groups, and to predict the metabolic function of the microbial flora. At the same time, the apparent digestibility of nutrients, digestive enzyme activity, daily weight gain and survival rate were also measured. TGEV infection could cause the imbalance of intestinal microbes in piglets, and increase of the relative abundance of Proteobacteria, and decrease of the relative abundance of Firmicutes, Bacteroidetes and Actinobacteri. With the addition of APB-13, this problem can be alleviated, which can reduce the relative abundance of Proteobacteria and improve the balance of intestinal microorganisms. At the microbial genus level, after adding APB-13, the relative abundance of Catenibacterium, Enterobacter and Streptococcus in the intestinal tract of piglets infected with TGEV showed significant decrease, while the relative abundance of Lactobacillus and Ruminococcus increased. Finally, we found that APB-13 can significantly increase the activity of digestive enzyme in the intestinal tract of piglet, thereby improving the apparent digestibility of nutrients and the growth performance of piglets. This study demonstrates that APB-13 can alleviate the adverse outcomes caused by TGEV infection by correcting the intestinal microbial disorders.

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Section: Discussionmentioning

confidence: 93%

APB‐13 improves the adverse outcomes caused by TGEV infection by correcting the intestinal microbial disorders in piglets

Liang

Wang

Lian

et al. 2021

Animal Physiology Nutrition

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“…peripartum period (20,21). The previous research found that adding antimicrobial peptides to the diet reduced the pH value in the intestine and increased the abundance of Firmicutes such as lactic acid bacteria (6,22). It is noteworthy that the abundance of Butyrivibrio genus related to butyric acids is decreased in the present study, and there was an upper trend in the milk yield at the end of the experiment in goats that received the supplementation of BLFc.…”

Section: Table 3 |mentioning

confidence: 45%

“…Antimicrobial peptides promote the abundance of Firmicutes and Tenericutes and increase LF synthesis in the peripartum period ( 20 , 21 ). The previous research found that adding antimicrobial peptides to the diet reduced the pH value in the intestine and increased the abundance of Firmicutes such as lactic acid bacteria ( 6 , 22 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Supplementation of Bovine Lactoferricin on Rumen Microbiota, Lactation, and Health in Dairy Goats

et al. 2021

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This study aimed to investigate the biological effects of supplementation of bovine lactoferricin (BLFc) at the rate of 100 mg/kg/day (LF-1) or 200 mg/kg/day (LF-2) in lactating dairy goats. Dietary BLFc supplementation increased the concentration of lactoferrin (LF) in the milk and serum (p < 0.05) without affecting the feed intake. In the LF-1 group, serum Fe, total antioxidant (T-AOC), and immunoglobulin A (IgA) were increased (p < 0.05), while malondialdehyde (MDA) was decreased (p < 0.05). In the LF-2 group, ruminal fluid pH value was decreased (p < 0.05), and the composition of ruminal microflora on day 42 was more diversified. Firmicutes phylum in the LF-2 group was the most abundant phyla. In contrast, Bacteroidetes phylum in the control group and the LF-1 group were the most abundant. Lower milk somatic cell count and higher IgA were observed in the LF-1 group and the LF-2 group than those in the control group (p < 0.05). These results suggested beneficial effects of supplementation of 100 mg/kg/day BLFc on reducing the oxidative stress and altering diversity of ruminal microflora.

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“…In the present study, we randomly classified 32 Duroc × Landrace × Yorkshire (DLY) female weaned piglets into two groups, either raised with spontaneous microbial colonization as the control (CON) group or treated with two types of broad-spectrum LDA, i.e., chlortetracycline and virginiamycin (see Methods). During the 4 weeks of the experimental period, growth phenotypes and diarrhoea index (DI) [ 18 , 19 ] for all the DLY piglets were gathered in each week (Table S 1 ) and analysed by SPSS 20.0 (SPSS, Inc.) with a two-tailed independent t-test. During 4 weeks of treatment, a growth-promoting effect was observed starting from the first week, as exemplified by the elevated body weight (BW) and average daily gain (ADG) of the LDA group.…”

Section: Resultsmentioning

confidence: 99%

Multi-omics profiling highlights lipid metabolism alterations in pigs fed low-dose antibiotics

Zhang

Liu

et al. 2020

BMC Genet

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Background In order to study the relations of hepatocellular functions, weight gain and metabolic imbalance caused by low-dose antibiotics (LDA) via epigenetic regulation of gene transcription, 32 weaned piglets were employed as animal models and randomly allocated into two groups with diets supplemented with 0 or LDA (chlorotetracycline and virginiamycin). Results During the 4 weeks of the experiment, LDA showed a clear growth-promoting effect, which was exemplified by the significantly elevated body weight and average daily gain. Promoter methylome profiling using liquid hybridization capture-based bisulfite sequencing (LHC-BS) indicated that most of the 745 differential methylation regions (DMRs) were hypermethylated in the LDA group. Several DMRs were significantly enriched in genes related with fatty acids metabolic pathways, such as FABP1 and PCK1. In addition, 71 differentially expressed genes (DEGs) were obtained by strand-specific transcriptome analysis of liver tissues, including ALOX15, CXCL10 and NNMT, which are three key DEGs that function in lipid metabolism and immunity and which had highly elevated expression in the LDA group. In accordance with these molecular changes, the lipidome analyses of serum by LC-MS identified 38 significantly differential lipids, most of which were downregulated in the LDA group. Conclusions Our results indicate that LDA could induce epigenetic and transcriptional changes of key genes and lead to enhanced efficiency of lipid metabolism in the liver.

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Effects of composite antimicrobial peptide on growth performance and health in weaned piglets

Cited by 12 publications

References 30 publications

APB‐13 improves the adverse outcomes caused by TGEV infection by correcting the intestinal microbial disorders in piglets

APB‐13 improves the adverse outcomes caused by TGEV infection by correcting the intestinal microbial disorders in piglets

Effects of Dietary Supplementation of Bovine Lactoferricin on Rumen Microbiota, Lactation, and Health in Dairy Goats

Multi-omics profiling highlights lipid metabolism alterations in pigs fed low-dose antibiotics

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