Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesis

Abstract

Bone tissue with strong adaptability is often in a complex dynamical microenvironment in vivo, which is associated with the pathogenesis and treatment of orthopedic diseases. Therefore, it is of great significance to investigate the effects of corresponding compound stimulation on cell behaviors. Herein, a fluid shear stress (FSS) plus ultrasound stimulation platform suitable for cell studies based on a microfluidic chip was constructed and bone marrow mesenchymal stem cell (BMSC) was chosen as a model cell. The proliferation and osteogenesis of BMSCs under the compound stimulation of FSS plus ultrasound in growth medium without any soluble induction factors were firstly investigated. Single FSS stimulation and static culture conditions were also examined. Results illustrated that suitable single FSS stimulation (about 0.06 dyn/cm2) could significantly enhance cell proliferation and osteogenesis simultaneously when compared to the static control, while greater FSS mitigated or even restricted these enhancing effects. Interestingly, ultrasound stimulation combined with this suitable FSS stimulation further accelerated cell proliferation as the intensity of ultrasound increasing. As for the osteogenesis under compound stimulation, it was relatively restricted under lower ultrasound intensity (about 0.075 W/cm2), while promoted when the intensity became higher (about 1.75 W/cm2). This study suggests that both the cell proliferation and osteogenesis are very responsive to the magnitudes of FSS and ultrasound stimulations and can be both significantly enhanced by proper combination strategies. Moreover, these findings will provide valuable references for the construction of effective cell bioreactors and also the treatment of orthopedic diseases.

Development of biodegradable Zn-Mn-Li and CaP coatings on Zn-Mn-Li alloys and cytocompatibility evaluation for bone graft

“…However, the spread area of osteoblasts on CaP Zn0.8Mn0.1Li was larger, and the aspect ratio was about 2:1. Relevant studies had also confirmed that the cytoskeleton guides cells to regulate their adhesion or other behaviors to adapt to the corresponding microenvironment ( Jing et al, 2021 ). It had been reported that more cell contact and larger cell spread are conducive to osteogenic differentiation of MC3T3-E1 cells ( Yao et al, 2013c ).…”

One-Step Approach to Prepare Transparent Conductive Regenerated Silk Fibroin/PEDOT:PSS Films for Electroactive Cell Culture

“…The RSF/PEDOT:PSS films were cut into 1.5 × 1.5 cm 2 squares and placed at the bottom of a 24-well plate. The films were treated by plasma (the power, gas flow rate, and treating time were fixed as 800 W, 0.8 L/min, and 2 min, respectively) and fibronectin (25 μg/mL, 500 μL/well, and 12 h incubation) before cell seeding to achieve good cell adhesion ability. One milliliter of PC12 cell suspension with a concentration of 3 × 10 4 cells/mL was added on each film sample.…”

“…Ryan et al reported a kind of conductive degummed natural silk fiber modified by immersion of PEDOT:PSS . The RSF materials are more versatile than the degummed natural silk fibers as the shape and structure of the RSF materials could be arbitrarily designed and well fabricated through solution processing approaches. − However, RSF lacks the hierarchical fibrillar structure present in the degummed natural silk, which makes it difficult to construct an effective and stable conductive coating on RSF materials using the traditional immersion method, especially for the surface with low roughness. Besides, the strategy of blending RSF and PEDOT:PSS may show the relatively poor electroconductive property of the composite material due to the diluted CP density.…”

Recent advances in regenerative biomaterials

“…Yao et al . [ 434 ] developed a platform to investigate the influence of fluid shear stress plus ultrasound stimulation on the behavior of bone marrow MSCs. The results showed that cell differentiation was responsive to the magnitudes of fluid shear stress and ultrasound stimulations and thus significantly enhanced by proper combination strategies.…”