Effects of Concentration on Aggregation of BODIPY-Based Fluorescent Dyes Solution

8-[4-(2-Hydroxypropane-2-yl)phenyl]-1,3,4,4,5,7-hexamethyl-4-boron-3a,4a-diaza-S-indacene

“…The OH-group produced a broad singlet signal at 1.27 ppm. In the 13 C NMR spectrum, all signals of aromatic and heterocyclic fragments could be observed in range of 121-153 ppm, and alkyl fragments produce signals at 14-73 ppm region. However, the signals of B(CH3)2 fragment are not visible in the 13 C spectrum.…”

“…In the 13 C NMR spectrum, all signals of aromatic and heterocyclic fragments could be observed in range of 121-153 ppm, and alkyl fragments produce signals at 14-73 ppm region. However, the signals of B(CH3)2 fragment are not visible in the 13 C spectrum. The same peculiarity was previously described for other BODIPY containing B-C bond [11].…”

“…The maximum absorption wavelength in DMSO is λmax = 503 nm, with the absorbance 0.093, producing the molar extinction coefficient ε(λmax) = 93,000 M −1 cm −1 . In water with 1% DMSO, the absorption peak is blue-shifted and wider, probably indicating the formation of aggregates [12,13]. Figure 2B shows the emission spectra of the same solutions excited at 500 nm.…”

“…NMR spectra were recorded on Bruker Avance-300 (300. 13 MHz for 1 H) and Avance-400 (400.13 MHz for 1 H, 100.62 MHz 13 C) spectrometers; chemical shifts of 1 H and 13 C{ 1 H} are provided in ppm, with solvent signals serving as the internal standard ( 1 H = 7.24 ppm and 13 C = 77.16 ppm for CDCl3). The masses of molecular ions were determined by high-resolution mass spectrometry (HRMS) by means of a DFS Thermo Scientific instrument (EI, 70 eV).…”

“…The following are available online: copies of 1 H and13 C NMR spectra for all isolated compounds. Moskalensky A.E.…”