Effects of conditioned medium obtained from human adipose-derived stem cells on skin inflammation

Yano, Fumiko; Takeda, Taiga; Kurokawa, Toshiko; Tsubaki, Toshiya; Chijimatsu, Ryota; Tsuji, Shinsaku; Tsuji, Shinsaku; Tanaka, Sakae; Saito, Taku

doi:10.1016/j.reth.2022.03.009

Cited by 9 publications

(3 citation statements)

References 22 publications

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“…ASC primarily stimulate angiogenesis through paracrine secretions of angiopoietins, VEGF, FGF-2, PDGF, TGF-β, and HGF, 22-24 while also supporting angiogenesis through their own differentiation toward endothelial cells. 25 In addition to angiogenesis, ASC produce cell growth factors (such as FGFs, EGF, KGF, IGF) promoting the proliferation of dermal fibroblast and keratinocytes 26 leading to the dermo-epidermic reconstruction. They also secrete anti-fibrotic molecules 27 that could positively affect chronic wounds, express pattern recognition receptors against pathogens, such as Toll-like receptors, 28 and exert an anti-microbial activity.…”

Section: Mechanism Of Action and Asc Delivery On Woundsmentioning

confidence: 99%

Adipose-Derived Stromal Cells for Chronic Wounds: Scientific Evidence and Roadmap Toward Clinical Practice

Brembilla

Vuagnat

Boehncke

et al. 2022

Stem Cells Translational Medicine

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Chronic wounds, ie, non-healing ulcers, have a prevalence of ~1% in the general population. Chronic wounds strongly affect the quality of life and generate considerable medical costs. A fraction of chronic wounds will heal within months of appropriate treatment; however, a significant fraction of patients will develop therapy-refractory chronic wounds, leading to chronic pain, infection, and amputation. Given the paucity of therapeutic options for refractory wounds, cell therapy and in particular the use of adipose-derived stromal cells (ASC) has emerged as a promising concept. ASC can be used as autologous or allogeneic cells. They can be delivered in suspension or in 3D cultures within scaffolds. ASC can be used without further processing (stromal vascular fraction of the adipose tissue) or can be expanded in vitro. ASC-derived non-cellular components, such as conditioned media or exosomes, have also been investigated. Many in vitro and preclinical studies in animals have demonstrated the ASC efficacy on wounds. ASC efficiency appears to occurs mainly through their regenerative secretome. Hitherto, the majority of clinical trials focused mainly on safety issues. However more recently, a small number of randomized, well-controlled trials provided first convincing evidences for a clinical efficacy of ASC-based chronic wound therapies in humans. This brief review summarizes the current knowledge on the mechanism of action, delivery and efficacy of ASC in chronic wound therapy. It also discusses the scientific and pharmaceutical challenges to be solved before ASC-based wound therapy enters clinical reality.

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Section: Mechanism Of Action and Asc Delivery On Woundsmentioning

confidence: 99%

Adipose-Derived Stromal Cells for Chronic Wounds: Scientific Evidence and Roadmap Toward Clinical Practice

Brembilla

Vuagnat

Boehncke

et al. 2022

Stem Cells Translational Medicine

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“…The conditioned medium of human cord blood mesenchymal stem cells (USC-CM) has an anti-inflammatory effect through the growth factors and cytokines in it, such as EGF [142]. ASC-CM treatment was shown to promote the anti-inflammatory phenotype of macrophages, partially protecting the skin barrier damaged by PMA exposure, and has broad application prospects in wound healing and skin inflammation [143].…”

Section: Effects Of Conditioned Mediamentioning

confidence: 99%

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Wu,

Sun,

et al. 2024

Biomedicines

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Mesenchymal stem cells (MSCs) have been recognized as a cell therapy with the potential to promote skin healing. MSCs, with their multipotent differentiation ability, can generate various cells related to wound healing, such as dermal fibroblasts (DFs), endothelial cells, and keratinocytes. In addition, MSCs promote neovascularization, cellular regeneration, and tissue healing through mechanisms including paracrine and autocrine signaling. Due to these characteristics, MSCs have been extensively studied in the context of burn healing and chronic wound repair. Furthermore, during the investigation of MSCs, their unique roles in skin aging and scarless healing have also been discovered. In this review, we summarize the mechanisms by which MSCs promote wound healing and discuss the recent findings from preclinical and clinical studies. We also explore strategies to enhance the therapeutic effects of MSCs. Moreover, we discuss the emerging trend of combining MSCs with tissue engineering techniques, leveraging the advantages of MSCs and tissue engineering materials, such as biodegradable scaffolds and hydrogels, to enhance the skin repair capacity of MSCs. Additionally, we highlight the potential of using paracrine and autocrine characteristics of MSCs to explore cell-free therapies as a future direction in stem cell-based treatments, further demonstrating the clinical and regenerative aesthetic applications of MSCs in skin repair and regeneration.

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“…MSC therapy is likely to be effective for the treatment of various diseases such as osteoarthritis, cerebral infarction, spinal cord injury, chronic kidney disease, and cirrhosis, in addition to other degenerative and inflammatory diseases. (2) Developments in regenerative medicine are making it possible for patients or athletes to live without debilitating heart disease, to regrow cartilage in an injured knee, for injured soldiers to recover and Then and Now: Regenerative Medicine ---A Short Over view return to normal life, for children with congenital heart defects to receive a heart or valve patch that will grow as they do, and for researchers to finally understand the initiation and progression of various diseases of organs and other connective tissues. But the potential for stem cell medical therapies often seems to be matched only by the controversy the phrase sometimes inspires.…”

Section: Then and Now: Regenerative Medicine ---A Short Over Viewmentioning

confidence: 99%

Then and Now: Regenerative Medicine --- A Short Over view

2022

AHS

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This is an article on regenerative medicine about the current status and prospects of the field. Regenerative medicine is a branch of translational research which deals with the process of replacing, regenerating or engineering human cells, tissues, or organs to restore or establish normal function (1) Stem cells are cells with the ability to grow and differentiate into more than 200 cell types. The differentiation ability of all stem cell types would be stimulated to obtain specialized cells that represent renewable sources of functional cells useful for cell-based therapy.

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Effects of conditioned medium obtained from human adipose-derived stem cells on skin inflammation

Cited by 9 publications

References 22 publications

Adipose-Derived Stromal Cells for Chronic Wounds: Scientific Evidence and Roadmap Toward Clinical Practice

Adipose-Derived Stromal Cells for Chronic Wounds: Scientific Evidence and Roadmap Toward Clinical Practice

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

Then and Now: Regenerative Medicine --- A Short Over view

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