Effects of Conidia of Various Aspergillus Species on Apoptosis of Human Pneumocytes and Bronchial Epithelial Cells

Féménia, Françoise; Huet, D.; Lair-Fulleringer, Sybille; Wagner, Marie-Christine; Sarfati, J.; Shingarova, L. N.; Guillot, Jacques; Boireau, Pascal; Chermette, René; Berkova, Nadejda

doi:10.1007/s11046-008-9175-3

Cited by 15 publications

(20 citation statements)

References 35 publications

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“…These results are consistent with a previous study showing that culture filtrates from A. fumigatus did not cause membrane blebbing and apoptotic bodies in A549 cells and that the cells died from necrosis [44]. It has been shown, furthermore, that conidia from A. fumigatus isolated from humans, birds and the environment were able to inhibit apoptosis induced in A549 cells and 16HBE bronchial epithelial cells [45].…”

Section: Discussionsupporting

confidence: 93%

Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells

et al. 2012

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Inhalation of Aspergillus fumigatus conidia can cause severe aspergillosis in immunosuppressed people. A. fumigatus produces a large number of secondary metabolites, some of which are airborne by conidia and whose toxicity to the respiratory tract has not been investigated. We found that spores of A. fumigatus contain five main compounds, tryptoquivaline F, fumiquinazoline C, questin, monomethylsulochrin and trypacidin. Fractionation of culture extracts using RP-HPLC and LC-MS showed that samples containing questin, monomethylsulochrin and trypacidin were toxic to the human A549 lung cell line. These compounds were purified and their structure verified using NMR in order to compare their toxicity against A549 cells. Trypacidin was the most toxic, decreasing cell viability and triggering cell lysis, both effects occurring at an IC50 close to 7 µM. Trypacidin toxicity was also observed in the same concentration range on human bronchial epithelial cells. In the first hour of exposure, trypacidin initiates the intracellular formation of nitric oxide (NO) and hydrogen peroxide (H2O2). This oxidative stress triggers necrotic cell death in the following 24 h. The apoptosis pathway, moreover, was not involved in the cell death process as trypacidin did not induce apoptotic bodies or a decrease in mitochondrial membrane potential. This is the first time that the toxicity of trypacidin to lung cells has been reported.

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Section: Discussionsupporting

confidence: 93%

Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells

et al. 2012

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“…The larger diameter of A. flavus conidia (3.5–4.5 μ m) [4] may explain their lower pathogenicity when compared to A. fumigatus in experimental infections [80]. In a recent study [81], the effects of Aspergillus conidia on human respiratory cell apoptosis was evaluated. A. fumigatus and A. flavus conidia inhibited cellular apoptosis, while A. nidulans , A. niger , and A. oryzae conidia did not.…”

Section: Prerequisites To Avian Aspergillosis Onsetmentioning

confidence: 99%

“…A. fumigatus and A. flavus conidia inhibited cellular apoptosis, while A. nidulans , A. niger , and A. oryzae conidia did not. However, there were no differences in the inhibition of apoptosis by A. fumigatus conidia from either human, avian, or environmental isolates [81]. …”

Section: Prerequisites To Avian Aspergillosis Onsetmentioning

confidence: 99%

Aspergillus fumigatusin Poultry

Arné

Thierry

Wang

et al. 2011

International Journal of Microbiology

113

116

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Aspergillus fumigatus remains a major respiratory pathogen in birds. In poultry, infection by A. fumigatus may induce significant economic losses particularly in turkey production. A. fumigatus develops and sporulates easily in poor quality bedding or contaminated feedstuffs in indoor farm environments. Inadequate ventilation and dusty conditions increase the risk of bird exposure to aerosolized spores. Acute cases are seen in young animals following inhalation of spores, causing high morbidity and mortality. The chronic form affects older birds and looks more sporadic. The respiratory tract is the primary site of A. fumigatus development leading to severe respiratory distress and associated granulomatous airsacculitis and pneumonia. Treatments for infected poultry are nonexistent; therefore, prevention is the only way to protect poultry. Development of avian models of aspergillosis may improve our understanding of its pathogenesis, which remains poorly understood.

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“…Infected vessels of lung can cause formation of thrombosis and hemorrhagic infarction. Therefore, ground-glass-like changes, nodules and cavity with inserted nodules are widely fund, but cavity without inserted nodule is seldom observed in clinical [18,19].…”

Section: Discussionmentioning

confidence: 99%

Preliminary Study of Fourteen AIDS-Associated Lung Complications from Imaging,Pathology and Microbiology

Xia¹,

Hu²,

Wang³

et al. 2017

J Clin Case Rep

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HIV-related pulmonary complication is a serious problem in the worldwide. In order to understand features of AIDS-associated pulmonary complications in clinical. Imaging features, microbe characterization and pathology of 14 AIDS-associated pulmonary disease patients derived from Nanyang Medical College hospitals were measured by corresponding techniques. 14 patients infected by different virus, fungus and bacteria showed special features of image and pathology, respectively. Regarding of various clinical characterizations derived from AIDS-associated pulmonary complications, combination different strategy is contributing to elucidate characterizations of AIDSassociated pulmonary complications.

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Effects of Conidia of Various Aspergillus Species on Apoptosis of Human Pneumocytes and Bronchial Epithelial Cells

Cited by 15 publications

References 35 publications

Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells

Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells

Aspergillus fumigatusin Poultry

Preliminary Study of Fourteen AIDS-Associated Lung Complications from Imaging,Pathology and Microbiology

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