Effects of contaminants of emerging concern on Myzus persicae (Sulzer, Hemiptera: Aphididae) biology and on their host plant, Capsicum annuum

Pennington, Marcus J.; Rothman, Jason A.; Jones, Michael B.; McFrederick, Quinn S.; Gan, Jay; Trumble, John T.

doi:10.1007/s10661-018-6503-z

Cited by 15 publications

(14 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We prepared 16S rRNA gene libraries for paired-end Illumina MiSeq sequencing for each individual bee (N=134) using the protocol from McFrederick and Rehan 2016 (61), Pennington et al 2018 (62) and Rothman et al 2018 (33). We incorporated the 16S rRNA gene primer sequence, unique barcode sequence, and Illumina adapter sequence as in (63).…”

Section: Methodsmentioning

confidence: 99%

The direct and indirect effects of environmental toxicants on the health of bumble bees and their microbiomes

Rothman

Russell

Leger

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Bumble bees (Bombus spp.) are important and widespread insect pollinators, but the act of foraging on flowers can expose them to harmful pesticides and environmental chemicals such as oxidizers and heavy metals. How these compounds directly influence bee survival and indirectly affect bee health via the gut microbiome is largely unknown. As the toxins and toxicants in floral nectar and pollen take many forms, we explored the genomes of core bumblebee microbes (Using RAST) for their potential to detoxify cadmium, copper, selenium, the neonicotinoid pesticide imidacloprid, and hydrogen peroxide - which have all been identified in floral nectar and pollen. We then exposed Bombus impatiens workers to varying concentrations of these chemicals spiked into their diet and identified the direct effects on bee survival. Using field realistic doses, we further explored indirect effects on bee microbiomes. We found multiple genes in core gut microbes that have the potential to aid in detoxifying harmful chemicals. We also found that while the chemicals are largely toxic at levels within and above field-realistic concentrations, the field-realistic concentrations - except for imidacloprid - altered the composition of the bee microbiome, potentially causing gut dysbiosis. Overall, our study shows that environmental chemicals found in floral nectar and pollen can directly cause bee mortality, and at field-realistic levels, likely have indirect, deleterious effects on bee health via their influence on the bee microbiome.

show abstract

Section: Methodsmentioning

confidence: 99%

The direct and indirect effects of environmental toxicants on the health of bumble bees and their microbiomes

Rothman

Russell

Leger

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…We used a modified DNA extraction protocol based on the study by Engel and colleagues () and Pennington and and colleagues (; ). Using sterile technique, we dissected whole guts out of each bee and placed them into 96‐well bead‐beating plates (Qiagen, Valencia, CA) containing 50–100 μl of 0.1 mm glass beads, one 3.4 mm steel‐chrome bead (Biospec, Bartlesville, OK), and Qiagen lysis buffer, then homogenized the mixture with a Qiagen Tissuelyser at 30 Hz for 6 min.…”

Section: Methodsmentioning

confidence: 99%

The bumble bee microbiome increases survival of bees exposed to selenate toxicity

Rothman

Leger

Graystock

et al. 2019

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Summary Bumble bees are important and widespread insect pollinators who face many environmental challenges. For example, bees are exposed to the metalloid selenate when foraging on pollen and nectar from plants growing in contaminated soils. As it has been shown that the microbiome of animals reduces metalloid toxicity, we assayed the ability of the bee microbiome to increase survivorship against selenate challenge. We exposed uninoculated or microbiota‐inoculated Bombus impatiens workers to a field‐realistic dose of 0.75 mg l−1 selenate and found that microbiota‐inoculated bees survive slightly but significantly longer than uninoculated bees. Using 16S rRNA gene sequencing, we found that selenate exposure altered gut microbial community composition and relative abundance of specific core bacteria. We also grew two core bumble bee microbes – Snodgrassella alvi and Lactobacillus bombicola – in selenate‐spiked media and found that these bacteria grew in the tested concentrations of 0.001–10 mg l−1 selenate. Furthermore, the genomes of these microbes harbour genes involved in selenate detoxification. The bumble bee microbiome slightly increases survivorship when the host is exposed to selenate, but the specific mechanisms and colony‐level benefits under natural settings require further study.

show abstract

“…We used a modified DNA extraction protocol based on Engel et al 2013 [37], Pennington et al 2017 [38], and Pennington et al 2018 [39]. We used sterile technique to remove the entire pollen provision of a M. rotundata brood cell and transferred it into 96-well tissue lysis plates (Qiagen, Valencia, CA).…”

Section: Dna Extraction and Amplicon Sequencing Library Preparationmentioning

confidence: 99%

Floral and Foliar Source Affect the Bee Nest Microbial Community

et al. 2018

Self Cite

View full text Add to dashboard Cite

Managed pollinators such as the alfalfa leafcutting bee, Megachile rotundata, are essential to the production of a wide variety of agricultural crops. These pollinators encounter a diverse array of microbes when foraging for food and nest-building materials on various plants. To test the hypothesis that food and nest-building source affects the composition of the bee-nest microbiome, we exposed M. rotundata adults to treatments that varied both floral and foliar source in a 2 × 2 factorial design. We used 16S rRNA gene and internal transcribed spacer (ITS) sequencing to capture the bacterial and fungal diversity of the bee nests. We found that nest microbial communities were significantly different between treatments, indicating that bee nests become inoculated with environmentally derived microbes. We did not find evidence of interactions between the fungi and bacteria within our samples. Furthermore, both the bacterial and fungal communities were quite diverse and contained numerous exact sequence variants (ESVs) of known plant and bee pathogens that differed based on treatment. Our research indicates that bees deposit plantassociated microbes into their nests, including multiple plant pathogens such as smut fungi and bacteria that cause blight and wilt. The presence of plant pathogens in larval pollen provisions highlights the potential for bee nests to act as disease reservoirs across seasons. We therefore suggest that future research should investigate the ability of bees to transmit pathogens from nest to host plant.

show abstract

Effects of contaminants of emerging concern on Myzus persicae (Sulzer, Hemiptera: Aphididae) biology and on their host plant, Capsicum annuum

Cited by 15 publications

References 63 publications

The direct and indirect effects of environmental toxicants on the health of bumble bees and their microbiomes

The direct and indirect effects of environmental toxicants on the health of bumble bees and their microbiomes

The bumble bee microbiome increases survival of bees exposed to selenate toxicity

Floral and Foliar Source Affect the Bee Nest Microbial Community

Contact Info

Product

Resources

About