2008
DOI: 10.1016/j.brainres.2008.06.074
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Effects of continuous hypoxia on energy metabolism in cultured cerebro-cortical neurons

Abstract: Mechanisms underlying hypoxia-induced neuronal adaptation have not been fully elucidated. In the present study we investigated glucose metabolism and the activities of glycolytic and TCA cycle enzymes in cerebro-cortical neurons exposed to hypoxia (3 days in 1% of O 2 ) or normoxia (room air). Hypoxia led to increased activities of LDH (251%), PK (90%), and HK (24%) and decreased activities of CS (15%) and GDH (34%). Neurons were incubated with [1-13 C]glucose for 45 and 120 min under normoxic or hypoxic (120 … Show more

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Cited by 28 publications
(24 citation statements)
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“…The use of young cultures (3–5 days after isolation) is essential, since mature cultured neurons exhibit significant and permanent adaptations in their energy metabolism [12]. Cerebellar granule cells loaded with fluo-3 were stimulated by brief pulses (≤ 10 s) of 60 mM K + ACSF.…”
Section: Resultsmentioning
confidence: 99%
“…The use of young cultures (3–5 days after isolation) is essential, since mature cultured neurons exhibit significant and permanent adaptations in their energy metabolism [12]. Cerebellar granule cells loaded with fluo-3 were stimulated by brief pulses (≤ 10 s) of 60 mM K + ACSF.…”
Section: Resultsmentioning
confidence: 99%
“…For example, cells are normally cultured under 95% air (20% O 2 ) and 5% CO 2 whereas cells in the normal brain are exposed to much lower levels of O 2 closer to around 2–5% [34]. These relatively low levels of physiological O 2 are accompanied by expression of hypoxia inducible factor 1 (HIF1), which transcriptionally activates the expression of many genes that includes several coding for glycolytic enzymes and glucose transporters [35];[36];[35];[36];[37]. The expression of different isoforms of monocarboxylic acid transporters (MCT), which transport pyruvate, lactate, and ketone bodies, can also be influenced by HIF1 [38] and by ambient levels of different fuels, including ketone bodies [39].…”
Section: Discussionmentioning
confidence: 99%
“…To address the impact of cooling to normalize the state of brain tissue under hypoxia, the redox ratio of coronal brain slices was measured under different oxygen tensions. The induction of hypoxic conditions is expected to diminish oxidative phosphorylation and shift the metabolic reactions towards glycolysis [63,69]. This shift is expected to lead to a reduction in FAD fluorescence signals, an increase in NADH fluorescence signals, and lowering the redox ratio.…”
Section: Resultsmentioning
confidence: 99%