Effects of conventionally-treated and ozonated wastewater on mortality, physiology, body length, and behavior of embryonic and larval zebrafish (Danio rerio)

Rothe, L.; Botha, Tarryn L.; Feld, Christian K.; Weyand, Michael; Zimmermann, Sonja; Smit, Nico J.; Wepener, Victor; Sures, Bernd

doi:10.1016/j.envpol.2021.117241

Cited by 11 publications

(5 citation statements)

References 64 publications

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“…In this study spanning 4 months, mortality was higher in the effluent-exposed group compared to the control. Controversial results had been found in previous reports ( Han Y. et al, 2021 ; Rothe et al, 2021 ). Effects of chronic toxicity on survival rate was showed in this study.…”

Section: Resultsmentioning

confidence: 82%

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

et al. 2021

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The ever-increasing production and processing of textiles will lead to greater risks of releasing pollutants into the environment. Textile wastewater treatment plants (TWTPs) effluent are an important source of persistent toxic pollutants in receiving water bodies. The effects of specific pollutants on organisms are usually studied under laboratory conditions, and therefore, comprehensive results are not obtained regarding the chronic combined effects of pollutants under aquatic environmental conditions. Thus, this study aimed to determine the combined effects of TWTP effluents on the growth performance, oxidative stress, inflammatory response, and intestinal microbiota of adult zebrafish (Danio rerio). Exposure to TWTP effluents significantly inhibited growth, exacerbated the condition factor, and increased the mortality of adult zebrafish. Moreover, markedly decreases were observed in the activities of antioxidant enzymes, such as CAT, GSH, GSH-Px, MDA, SOD, and T-AOC, mostly in the intestine and muscle tissues of zebrafish after 1 and 4 months of exposure. In addition, the results demonstrated that TWTP effluent exposure affected the intestinal microbial community composition and decreased community diversity. Slight changes were found in the relative abundance of probiotic Lactobacillus, Akkermansia, and Lactococcus in zebrafish guts after chronic TWTP effluent exposure. The chronic toxic effects of slight increases in opportunistic pathogens, such as Mycoplasma, Stenotrophomonas, and Vibrio, deserve further attention. Our results reveal that TWTP effluent exposure poses potential health risks to aquatic organisms through growth inhibition, oxidative stress impairment of the intestine and muscles, and intestinal microbial community alterations.

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Section: Resultsmentioning

confidence: 82%

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

et al. 2021

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“…Afterward, treated wastewater from the municipal wastewater treatment plant Schwerte (North Rhine‐Westphalia, Germany) was filled into three systems to reach a percentage of 33% treated wastewater (~120 L) per mesocosm after removing the same volume of control water beforehand in treatment systems. The wastewater treatment plant (WWTP) operated as explained in Rothe et al (2021) with the following measurements at the effluent on the day of sampling: turbidity 3.4 NTU; chemical oxygen demand 16 mg/L; total phosphorus 0.23 mg/L; temperature 9.1°C; pH 6.81; and conductivity 928 µS/cm.…”

Section: Methodsmentioning

confidence: 99%

“…The wastewater treatment plant (WWTP) operated as explained in Rothe et al (2021) with the following measurements at the effluent on the day of sampling: turbidity 3.4 NTU; chemical oxygen demand 16 mg/L; total phosphorus 0.23 mg/L; temperature 9.1°C; pH 6.81; and conductivity 928 µS/cm. Thus, three systems served as controls ("C") and three systems as treatments ("T").…”

Section: Study Design and Mesocosm Setupmentioning

confidence: 99%

Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater

et al. 2023

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Microbial communities in freshwater streams play an essential role in ecosystem functioning via biogeochemical cycling. Yet, the impacts of treated wastewater influx into stream ecosystems on microbial strain diversity remain mostly unexplored. Here, we coupled full‐length 16S ribosomal RNA gene Nanopore sequencing and strain‐resolved metagenomics to investigate the impact of treated wastewater on a mesocosm system (AquaFlow) run with restored river water. Over 10 days, community Bray–Curtis dissimilarities between treated and control mesocosm decreased (0.57 ± 0.058 to 0.26 ± 0.046) based on ribosomal protein S3 gene clustering, finally converging to nearly identical communities. Similarly, strain‐resolved metagenomics revealed a high diversity of bacteria and viruses after the introduction of treated wastewater; these microbes also decreased over time resulting in the same strain clusters in control and treatment at the end of the experiment. Specifically, 39.2% of viral strains detected in all samples were present after the introduction of treated wastewater only. Although bacteria present at low abundance in the treated wastewater introduced additional antibiotic resistance genes, signals of naturally occurring ARG‐encoding organisms resembled the resistome at the endpoint. Our results suggest that the previously stressed freshwater stream and its microbial community are resilient to a substantial introduction of treated wastewater.

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“…and their metabolites, as well as nutrients, salts, various metals, organometallic compounds and others are known (e.g. [74,75]). The impact of pollutants as stressors on parasites and their associated hosts depends largely on their concentration.…”

Section: Pollutionmentioning

confidence: 99%

Environmental parasitology: stressor effects on aquatic parasites

Sures

Nachev

Schwelm

et al. 2023

Trends in Parasitology

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Effects of conventionally-treated and ozonated wastewater on mortality, physiology, body length, and behavior of embryonic and larval zebrafish (Danio rerio)

Cited by 11 publications

References 64 publications

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

Effect of Chronic Exposure to Textile Wastewater Treatment Plant Effluents on Growth Performance, Oxidative Stress, and Intestinal Microbiota in Adult Zebrafish (Danio rerio)

Temporal disturbance of a model stream ecosystem by high microbial diversity from treated wastewater

Environmental parasitology: stressor effects on aquatic parasites

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