Effects of copper and cadmium on development and superoxide dismutase levels in horseshoe crab (Limulus polyphemus) embryos

Hamilton, Mary G.; Esposito, C. R.; Malin, Mia; Cusumano, Lucas R.; Botton, Mark L.

doi:10.1186/s40064-015-1267-1

Cited by 10 publications

(3 citation statements)

References 40 publications

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“…1a, b, the increased levels of MDA and LDH induced by HCY were significantly reduced by catalpol. GSH, as a co-factor for glutathione peroxidase and the redox enzyme, catalyzes the reduction of lipid peroxidation [21]. The GSH redox cycle involves in ROS scavenging and is considered as the most important anti-oxidant system in endothelial cells.…”

Section: Resultsmentioning

confidence: 99%

Catalpol Inhibits Homocysteine-induced Oxidation and Inflammation via Inhibiting Nox4/NF-κB and GRP78/PERK Pathways in Human Aorta Endothelial Cells

Wang

Jin

et al. 2018

Inflammation

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Hyperhomocysteinemia (HHCY) has been recognized as an independent risk factor for atherosclerosis and plays a vital role in the development of atherosclerosis. Catalpol, an iridoid glucoside extracted from the root of Rehmannia glutinosa , can produce anti-inflammatory, anti-oxidant, anti-tumor, and dopaminergic neurons protecting effects. This study aimed to determine the protecting effects of catalpol against homocysteine (HCY)-induced injuries in human aortic endothelial cells (HAECs) and uncover the underlying mechanisms: 1. HAECs were cultured with different concentrations of HCY (3 mM) and catalpol (7.5 μΜ, 15 μΜ, 30 μΜ) for 24 h. (1) The level of MDA and GSH as well as LDH release was measured with colorimetric assay. (2) Reactive oxygen species (ROS) were detected by flow cytometry analysis. (3) Western blotting analysis was performed to detect the expression of Nox4, p22 phox , ICAM-1, MCP-1, VCAM-1, IκB, nucleus p65, p65 phosphorylation, caspase-3, −9, bax, bcl-2, and ER stress-related proteins. (4) The expressions of CHOP, ATF4 were measured by qRT-PCR. (5) Mitochondrial membrane potential in HCY-treated HAECs was measured by rhodamine 123 staining, and the samples were observed by confocal laser scanning microscopy. 2. DPI, PDTC, and TUDCA were used to determine the interaction among Nox4/ROS, NF-κB, and endoplasmic reticulum stress. 3. TUDCA or Nox4 siRNA were used to investigate whether the effect of catalpol inhibiting the over-production of ROS were associated with inhibiting ER stress and Nox4 expression. Catalpol significantly suppressed LDH release, MDA level, and the reduction of GSH. Catalpol reduced HCY-stimulated ROS over-generation, inhibited the NF-κB transcriptional activation as well as the protein over-expressions of Nox4, ICAM-1, VCAM-1, and MCP-1. Catalpol elevated bcl-2 protein expression and reduced bax, caspase-3, −9 protein expressions in the HCY-treated HAECs. Simultaneously, catalpol could also inhibit the activation of ER stress-associated sensors GRP78, IRE1α, ATF6, P-PERK, P-eIF2α, CHOP, and ATF4 induced by HCY. In addition, the extent of catalpol inhibiting ROS over-generation and NF-κB signaling pathway was reduced after inhibiting Nox4 or ER stress with DPI or TUDCA. The inhibitor of NF-κB PDTC also reduced the effects of catalpol inhibiting the expressions of Nox4 and GRP78. Furthermore, the effect of catalpol inhibiting the over-generation of ROS was reduced by Nox4 siRNA. Catalpol could ameliorate HCY-induced oxidation, cells apoptosis and inflammation in HAECs possibly by inhibiting Nox4/NF-κB and ER stress.

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Section: Resultsmentioning

confidence: 99%

Catalpol Inhibits Homocysteine-induced Oxidation and Inflammation via Inhibiting Nox4/NF-κB and GRP78/PERK Pathways in Human Aorta Endothelial Cells

Wang

Jin

et al. 2018

Inflammation

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“…The increase of the activity or expression of SOD was shown earlier for the mollusk Ruditapesdecussatusexposed to a 4--100 µg/LCd 2+ [29] and for the freshwater crab Sinopotamonyangtsekienseafter exposure to 7.2 and 14.5 µg/L of Cd 2+ [30]. Hamilton et al [31], in turn, report the absence of well-defined relationship between Cd concentration and the SOD in the embryos of the horseshoe-shaped crab (Limulus polyphemus). It has been show…”

Section: Discussionmentioning

confidence: 81%

Antioxidant System Response of Freshwater Mussel Anodontacygnea to Cadmium Exposure

Sukhovskaya

Borvinskaya

Kochneva

et al. 2020

KLS

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Cadmium is one of the widespread toxic substances being hazardous for man as it capable to enter from the environment into animal and plant tissues and spreading along the food chain. We have studied the effect of Cd on the gills and hepatopancreas of the fresh water bivalve mollusks Anodontacygnea. After 12-day acclimation the mollusks were kept for 24 and 72 hours in aquariums with Cd2+ concentrations of 10, 50 and 100 μg/L. Mass-spectrometric analysis has shown that Cd accumulation rate increased with increasing metal concentration in the water. At cadmium concentration of 100 μg/L the mollusk was capable of accumulating up to 0.44 μg of Cd per day. The accumulation of such high metal concentrations in the mollusk tissues did not kill the animals, but signs of oxidative stress, more pronounced in the gills than in the digestive gland, were observed. Exposure to cadmium ions decreased GSH concentration and increase Рx activity in the mollusk gills as early as 24 hours after the beginning of the experiment. Changing the water in the aquarium had a considerable influence on SOD activity in the gills comparable with the effect of the addition of Cd.

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“…According to the literature, this is not specific to the considered species. In the horseshoe crab Limulus polyphemus , a high percentage of embryos that survive and hatch is generally unaffected by metal exposure, except at the highest copper concentration, even if there were significant delay in developmental rate (Hamilton et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Modulatory effect of the exudates released by the brown kelp Lessonia spicata on the toxicity of copper in early developmental stages of ecologically related organisms

Fellous

Andrade

Vidal-Ramirez

et al. 2016

Environ Sci Pollut Res

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Lessonia spicata is a key dominant species along the Pacific coast of South America, providing a habitat for many organisms. However, this role can be affected by abiotic stress, such as metals. To counteract the toxic effect, L. spicata, among other seaweeds, releases exudates that bind metals. In this study, tolerances to copper of organisms related to the kelp forest (spores of Ulva lactuca (Chlorophyceae) and L. spicata (Phaeophyceae) and Zoea I of Taliepus dentatus (Milne-Edwards, Crustacea)) were studied; then, exudates are assessed by their protective effect. Exudates increase the 48-h 50% effective concentration (EC50) of the germination of spores from 8 to 23 μg Cu L−1 for U. lactuca and from 119 to 213 μg Cu L−1 for L. spicata and the survival of the larvae Zoea I 48-h 50% of lethal concentration (LC50) from 144 to 249 μg Cu L−1. Results indicated that exudates had a protective effect. Each species is specifically sensitive to copper. Crab larvae Zoea I were able to support higher doses, and exposure before hatching increased their tolerance.

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Effects of copper and cadmium on development and superoxide dismutase levels in horseshoe crab (Limulus polyphemus) embryos

Cited by 10 publications

References 40 publications

Catalpol Inhibits Homocysteine-induced Oxidation and Inflammation via Inhibiting Nox4/NF-κB and GRP78/PERK Pathways in Human Aorta Endothelial Cells

Catalpol Inhibits Homocysteine-induced Oxidation and Inflammation via Inhibiting Nox4/NF-κB and GRP78/PERK Pathways in Human Aorta Endothelial Cells

Antioxidant System Response of Freshwater Mussel Anodontacygnea to Cadmium Exposure

Modulatory effect of the exudates released by the brown kelp Lessonia spicata on the toxicity of copper in early developmental stages of ecologically related organisms

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