Effects of Copper on the Burrowing Behavior of Estuarine and Coastal Invertebrates, the Polychaete<i>Nereis diversicolor</i>and the Bivalve<i>Scrobicularia plana</i>

Bonnard, Marc; Roméo, Michèle; Amiard‐Triquet, Claude

doi:10.1080/10807030802614934

Cited by 82 publications

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“…Tissues have potential defense mechanisms, including intracellular antioxidants and antioxidant enzymes [30] such as glutathione S-transferase (GST), superoxide dismutase (SOD), catalase (CAT), and metallothionein proteins (MTs). Our previous investigations [31] showed that activities/concentration of these biomarkers increased following exposure to AuNPs of different sizes on the marine bivalve Scrobicularia plana which is an intertidal deposit-feeder organism widely used in ecotoxicological studies [32,33]. With regard to these results, in the present study we explored the cellular impact of these gold nanoparticles on S. plana.…”

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Subcellular localization of gold nanoparticles in the estuarine bivalve Scrobicularia plana after exposure through the water

et al. 2013

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Nanoparticles are extensively used particularly in biomedical and industrial applications. Because of their colloidal stability, gold nanoparticles (AuNPs) are suspected being persistent in aquatic ecosystem. Thus, the potential toxicity of gold nanoparticles is addressed by using a bivalve model Scrobicularia plana. Using AuNPs in a range of sizes (5, 15, and 40 nm), we examined their subcellular localization in gills and digestive gland. Clams were exposed to AuNPs stabilized with citrate buffer and then diluted in seawater at the concentration of 100 μgL −1 .After 16 days water-borne exposure, using transmission electron microscopy, few particles were observed in gills, distributed as free in the cytoplasm, or associated with vesicles. In the digestive gland, the most striking feature was the presence of individual or small aggregates 40 nm sized within the nuclei colocalized with DNA. Depending on the size, individual or small aggregates (40 nm AuNPs) or more aggregated NPs (5 and 15 nm) were observed, with at least one of the dimensions (40-50 nm) allowing the passage through nuclear pores. Disorganization of chromatin was marked with an increase in filamentous structures. In some parts no chromatin was visible. Moreover, the perinuclear space from nuclei was enlarged in contaminated clams when compared to controls.

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Subcellular localization of gold nanoparticles in the estuarine bivalve Scrobicularia plana after exposure through the water

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“…This observation is in agreement with previous results obtained with N. diversicolor (Mouneyrac et al in press), showing that independent of any special event, the sediment quality is often lower in the Loire estuary. This cannot be attributed to differences in grain size (higher in the reference site than in the Loire estuary; see Table 2) since Bonnard et al (2009) have shown that burrowing in relatively coarse sediment required acclimation of the ragworms. Clear behavioural changes were detected in April, while nearly no differences were seen in May and June.…”

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“…They can significantly affect biogeochemical processes in sediments through bioturbation (Banta et al. 1999).Burrowing behaviour of polychaetes exposed to contaminated sediments was investigated by several authors, who highlighted the relationship between the aberration in locomotion and the effects of contaminants (Olla et al 1984, Burlinson & Lawrence 2007, Bonnard et al 2009). The ecological significance of reduced burrowing in contaminated sediments is obvious, as it makes the organisms more vulnerable to predator and wave action (Møhlenberg & Kiørboe 1983).…”

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“…Conversely, in April, weight at identical size was higher in ragworms from the Loire estuary. According to Spies et al (1988), responses of benthic populations to crude oil are similar to those expected from enrich- Bonnard et al (2009). Cross-tests were carried out in order to examine the causes of the behavioural disturbances.…”

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“…All the behavioural tests were set up in a dark, temperature-controlled room (16ºC) and conducted within 1 wk of collection. Burrowing experiments were carried out as described by Bonnard et al (2009). Briefly, we used plastic 100 ml containers filled with 5 cm of wet sediment from the site of origin.…”

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Assessment of the health status of populations of the ragworm Nereis diversicolor using biomarkers at different levels of biological organisation

Kálmán¹,

Palais²,

Amiard³

et al. 2009

Mar. Ecol. Prog. Ser.

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We compared behavioural (burrowing speed), biochemical (acetylcholinesterase [AChE] inhibition, activities of digestive enzymes) and physiological (feeding and egestion rates, condition) responses in Nereis diversicolor originating from a multipolluted estuary (Loire estuary) and a relatively clean site (Bay of Bourgneuf) on 3 occasions during spring 2008. Significant inhibition of AChE and digestive enzymes (amylase and carboxymethylcellulase) activities were recorded in individuals from the Loire estuary compared to the reference site. At the individual level no significant intersite differences in burrowing speed were observed when ragworms were exposed to their sediment of origin. No link between AChE activity and burrowing behaviour was found in worms from either site. Cross-tests revealed that behavioural disturbances in locomotion were not due to neurological dysfunction but to avoidance of contaminated sediments. Feeding and egestion rates were depressed significantly in worms originating from the Loire estuary compared to the reference site. These behavioural impairments were accompanied by changes in digestive enzyme activities, which can explain the generally poorer condition of ragworms from the Loire estuary. Temporal changes in responses reported in the present study underline the importance of the fluctuation of environmental factors and thus the need for more than one sampling series to assess the health status of a given site. Disturbances at the different levels of biological organisation are likely explained by environmental contamination either chronic in the Loire estuary or the result of a small oil spill. KEY WORDS: Nereis diversicolor · Estuarine sediment · Biomonitoring · Behavioural impairments · Biochemical biomarkers Resale or republication not permitted without written consent of the publisherMar Ecol Prog Ser 393: [55][56][57][58][59][60][61][62][63][64][65][66][67] 2009 of sediment toxicity tests to examine the impact of pollutants on the aquatic sedimentary environment (DelValls & Conradi 2000, Chapman et al. 2002. In addition, field studies provide information about the biological effects of pollutants at various levels of biological organisation.Although biochemical and physiological biomarkers have been used increasingly in environment toxicology, they are not yet able to predict the effects of contaminants at higher levels of biological organisation. The aim of most ecological risk assessments is to protect populations, communities and ecosystems; only rarely is the goal to protect individuals. Therefore it is important to study the link between the responses of biomarkers at the sub-organismal and supraorganismal levels (Forbes et al. 2006). Studies of biomarkers related to energy metabolism, growth or reproduction (De Coen & Janssen 2003, Durou et al. 2007b as well as behavioural disturbances (Dell'Omo 2002, Amiard-Triquet 2009) appear particularly promising. Behavioural biomarkers are sensitive tools that help us to assess the impact of contaminants at concent...

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Morphology of bivalves

Gosling¹

2015

Marine Bivalve Molluscs

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Effects of Copper on the Burrowing Behavior of Estuarine and Coastal Invertebrates, the PolychaeteNereis diversicolorand the BivalveScrobicularia plana

Cited by 82 publications

References 50 publications

Subcellular localization of gold nanoparticles in the estuarine bivalve Scrobicularia plana after exposure through the water

Subcellular localization of gold nanoparticles in the estuarine bivalve Scrobicularia plana after exposure through the water

Assessment of the health status of populations of the ragworm Nereis diversicolor using biomarkers at different levels of biological organisation

Morphology of bivalves

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