Effects of copper oxide nanomaterials (CuONMs) are life stage dependent – full life cycle in Enchytraeus crypticus

Bicho, Rita C.; Santos, Fátima C.F.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J.B.

doi:10.1016/j.envpol.2017.01.067

Cited by 56 publications

(61 citation statements)

References 36 publications

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“…The present study of Ni using the full life cycle test design confirms its potential to discriminate effects of nanomaterials not only at the single-organism level but also at the embryonic as well as other stages of the life cycle. The potential of the full life cycle test has already been confirmed for other materials: Ag NPs [24], CuO nanomaterials [28], and CdCl 2 [25]. Moreover, assessment of effects on the embryo stage is directly comparable to vertebrate models such as D. rerio [24].…”

Section: Discussionmentioning

confidence: 87%

Hazard assessment of nickel nanoparticles in soil—The use of a full life cycle test with Enchytraeus crypticus

Santos

Gomes

Scott-Fordsmand

et al. 2017

Enviro Toxic and Chemistry

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Nanoparticles (NPs) such as nickel (Ni) are widely used in several applications. Nevertheless, the environmental effects of Ni NPs are still poorly understood. In the present study, the toxicity of Ni NPs and nickel nitrate (NiNO ) was assessed using the standard test species in soil ecotoxicology, Enchytraeus crypticus (Oligochaeta), in a full life cycle test, adding the endpoints hatching, growth, and time to reach maturity, besides survival and reproduction as in the standard Organisation for Economic Co-operation and Development Guideline 220 and/or International Organization for Standardization 16387. For Ni NPs, the Ni in soil and in soil solution was concentration- and time-dependent, with a relatively higher soil solution content in the lower and shorter exposure concentrations and times. Overall, NiNO was more toxic than Ni NPs, and toxicity seemed to occur via different mechanisms. The former caused reduced hatching (50% effect concentration [EC50] = 39 mg Ni/kg soil), and the negative effects remained throughout the life cycle, in all measured endpoints (growth, maturation, survival, and reproduction). For Ni NPs, hatching was the most sensitive endpoint (EC50 = 870 mg Ni/kg soil), although the organisms recovered; that is, additional endpoints across the life cycle showed that this effect corresponded to a delay in hatching because organisms survived and reproduced at concentrations up to 1800 mg Ni/kg soil. On the other hand, the lowest tested concentration of Ni NPs (100 mg Ni/kg soil) caused reproduction effects similar to those at higher concentrations (1000 and 1800 mg Ni/kg soil). The present results show that the potential implications of a nonmonotonic dose response should be considered when assessing the risks of Ni NP exposure in soil. Environ Toxicol Chem 2017;36:2934-2941. © 2017 SETAC.

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Section: Discussionmentioning

confidence: 87%

Hazard assessment of nickel nanoparticles in soil—The use of a full life cycle test with Enchytraeus crypticus

Santos

Gomes

Scott-Fordsmand

et al. 2017

Enviro Toxic and Chemistry

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“…The CuCl 2 was obtained from Sigma-Aldrich (CAS number 10125-13-0) with a purity of 99%. The tested concentrations were selected based on the EC 50 for reproduction effect (CuCl 2 = 180 mg Cu/kg and CuO-NPs = 1400 mg Cu/kg soil dry weight) as known from previous Enchytraeid Reproduction Test (ERT) results 45 . CuCl 2 was added to pre moistened soil (20% w/w) as aqueous solution.…”

Section: Methodsmentioning

confidence: 99%

“…Further, the developed assay was used to study the longevity effects of CuO-NPs in comparison to CuCl 2 , using similar 50% effect concentrations on reproduction (EC 50 = 1400 and 180 mg Cu/kg for CuO-NPs and CuCl 2 , respectively) 45 .…”

Section: Introductionmentioning

confidence: 99%

Shorter lifetime of a soil invertebrate species when exposed to copper oxide nanoparticles in a full lifespan exposure test

Gonçalves

Gomes

Scott-Fordsmand

et al. 2017

Sci Rep

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Toxicity tests that last the all life duration of the organisms are not common, instead, long-term tests usually include one reproductive cycle. In the present study we optimized and propose a lifespan (all life) term test using Enchytraeus crypticus (Oligochaeta). The effect of copper oxide nanoparticles (CuO-NPs) was assessed in this lifespan test and compared to copper salt (CuCl2), using the same effect concentrations on reproduction (EC50). Monitored endpoints included survival and reproduction over-time (202 days). Results from survival showed that CuO-NPs caused shorter life of the adults compared to CuCl2 (control LT50: 218 days > CuCl2 LT50: 175 days > CuO-NPs LT50: 145 days). The effect was even more amplified in terms of reproduction (control ET50: 158 days > CuCl2 ET50: 138 days > CuO-NPs ET50: 92 days). Results suggest that CuO-NPs may cause a higher Cu effect via a trojan horse mechanism. The use of lifespan tests brings a novel concept in soil ecotoxicity, the longevity. This is a particularly important aspect when the subject is nanomaterials toxicity, where longer term exposure time is expected to reveal unpredicted effects via the current short/long-term tests. The present study confirms this higher effect for CuO-NPs.

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“…It has been given considerable focus and has currently an extensive suite of tools available for hazard assessment, far beyond the standard ecotoxicity test battery of many other species. The tools cover bioaccumulation (Amorim et al, 2011), avoidance (Bicho et al, 2015a), full life cycle test (Bicho et al, 2015b(Bicho et al, , 2016(Bicho et al, , 2017aSantos et al, 2017), embryotoxicity (Gonçalves et al, 2015), full life span (Gonçalves et al, 2017), multigenerational (Bicho et al, 2017b), multispecies (mesocosm) system (SMS) (Menezes-Oliveira et al, 2013), full transcriptome and microarray tool (Castro-Ferreira et al, 2014;Gomes et al, 2017), oxidative stress biomarkers (Ribeiro et al, 2015), energy metabolism and cellular energy allocation , genotoxicity via the comet assay (Maria et al, 2017), and metabolomics and proteomics. This species has further potential as it can be exposed via water for a short period and hence allows the assessment of other routes of exposure (Gomes et al, 2015b).…”

Section: Enchytraeid Toxicity Studies: Enchytraeus Crypticus/enchytramentioning

confidence: 99%

Nanomaterials in the Environment: Perspectives on in Vivo Terrestrial Toxicity Testing

Mendonça

Rizoli

Ávila

et al. 2017

Front. Environ. Sci.

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Over the last decade, engineered nanomaterials (NMs) brought a revolutionary development in many sectors of human life including electronics, paints, textiles, food, agriculture, and health care. However, the exponential growth in the number of NMs applications resulted in uncertainties regarding their environmental impacts. Currently, the common approach for assessing the toxicity of NMs such as, carbon-(fullerenes, single-and multi-walled carbon nanotubes), mineral-(gold and silver nanoparticles, cerium and zinc oxide, silicon and titanium dioxide), and organic-based NMs (dendrimers) includes standard guidelines applied to all chemical compounds. Nevertheless, NMs differ from traditional materials as their physicochemical and surface properties influence the toxic rather than their composition alone. Considering such NMs specificities, adaptations in some methods are necessary to ensure that environmental and human health risks are accurately investigated. In this context, the focus of this mini-review is to summarize the current knowledge in nanotoxicology regarding relevant organisms and experimental assays for assessing the terrestrial toxicity of NMs.

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Effects of copper oxide nanomaterials (CuONMs) are life stage dependent – full life cycle in Enchytraeus crypticus

Cited by 56 publications

References 36 publications

Hazard assessment of nickel nanoparticles in soil—The use of a full life cycle test with Enchytraeus crypticus

Hazard assessment of nickel nanoparticles in soil—The use of a full life cycle test with Enchytraeus crypticus

Shorter lifetime of a soil invertebrate species when exposed to copper oxide nanoparticles in a full lifespan exposure test

Nanomaterials in the Environment: Perspectives on in Vivo Terrestrial Toxicity Testing

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