Effects of Copper Oxide Nanoparticles on Tissue Accumulation and Antioxidant Enzymes of Galleria mellonella L.

Tunçsoy, Benay; Tunçsoy, Mustafa; Gomes, Tânia; Sousa, Vânia Serrão; Bebianno, Maria João; Özalp, Pınar

doi:10.1007/s00128-018-2529-8

Cited by 49 publications

(20 citation statements)

References 30 publications

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“…Prophylactic administration of free anidulafungin led to a less than ~40% survival of Galleria for all administration times investigated. While G. mellonella infections have been used to evaluate the activity of antifungal therapeutics (Kloezen et al, 2018; Li et al, 2013), limited studies have been performed that investigate administration of nanoparticles in a G. mellonella infection model (Sezer Tuncsoy et al, 2019). Here, we hypothesize that after infection, the liposomes improve localization to the C. albicans within the G. mellonella open circulation (Pass, 2000), similar to fungal cell localization results observed via flow cytometry in vitro.…”

Section: Discussionmentioning

confidence: 99%

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

Vera‐González

Bailey‐Hytholt

Langlois

et al. 2020

J Biomedical Materials Res

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Fungal infections can cause significant patient morbidity and mortality. Nanoparticle therapeutics have the potential to improve treatment of these infections. Here we report the development of liposomal nanoparticles incorporating anidulafungin, a potent antifungal, with the goal of increasing its solubility and aiding in localization to fungi. Liposomes were fabricated with three concentrations of anidulafungin yielding monodisperse ~100 nm unilamellar vesicles. All three formulations inhibited planktonic Candida albicans growth at a minimum inhibitory concentration equivalent to free drug. All three formulations also disrupted preformed C. albicans biofilms, reducing fungal burden by as much as 99%, exhibiting superior biofilm disruption compared with free drug. Liposome formulations tested in vivo in C. albicans infected Galleria mellonella wax moth larvae demonstrated increased survival compared to free drug equivalents, leading to a survival of 33 to 67% of larvae over 7 days depending on the liposome utilized compared with only 25% survival of larvae administered free drug. Liposomal formulations along with free anidulafungin did not cause red blood cell lysis. Ultimately, the liposome formulations reported here increased anidulafungin solubility, displayed promising efficacy against planktonic and biofilm C. albicans, and improved the survival of C. albicans–infected G. mellonella compared to free anidulafungin.

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Section: Discussionmentioning

confidence: 99%

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

Vera‐González

Bailey‐Hytholt

Langlois

et al. 2020

J Biomedical Materials Res

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“…% Inhibition = 100-100 × (GGT with NPs /GGT without NPs). (1) Several studies revealed the effects of copper oxide nanoparticles on tissue accumulation and antioxidant enzymes of Galleria Mellonella L. [30] and Oreochromis niloticus [31]. The previous study of Kojouri et al indicated the beneficial effects of Se NPs on antioxidant activity and weight gain patterns of newborn lambs which may promote lambs' growth rate in the postnatal period [32].…”

Section: Effect Of Cuo and Se Nanoparticles On Gamma-glutamyl Transferase Activitymentioning

confidence: 99%

Impact of Copper Oxide and Selenium Nanoparticles on the Activities of Myeloperoxidase and Gamma-Glutamyl Transferase Related Oxidative Stress of Myocardial Infarction Patients

Ahmed¹,

Hussain²,

Abdulsattar³

2021

Nano BioMed ENG

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Nanomedicine has tremendous prospects for the improvement of the diagnosis and treatment of human diseases. Nanotechnology has the potential to revolutionize a wide array of tools in biotechnology so that they are more personalized, portable, cheaper, safer and easier to administer. The present study aims to study the impact of copper oxide and selenium nanoparticles on the oxidative stress in sera of myocardial infraction patients through the synthesis and characterization of copper oxide and selenium nanoparticles, and the evaluation of the status of oxidative stress in sera of myocardial infraction patients through measurement of the activities of myeloperoxidase and gammaglutamyl transferase in the presence and absence of nanoparticles in sera of myocardial infraction patients. Cupric oxide nanoparticles and selenium nanoparticles have been prepared by a chemical method and by laser ablation method respectively, and have been characterized. The result indicated that copper oxide (grain shapes with the average size 30 -80 nm) and selenium nanoparticles (rodshaped structures with the average size 70 -90 nm) had an inhibition effects on myeloperoxidase and gamma-glutamyl transferase activities, and as a result had an inhibition effects on oxidative stress.

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“…The ability of these larvae to survive at 15-37 • C is very important [27,28]. Since the expression of virulence factors of many pathogenic microorganisms that threaten human health occurs at 37 • C, this fact brings G. mellonella larvae to the forefront in establishing treatment protocols against infectious diseases [24,29,30]. The hemolymph of G. mellonella larvae contains different types of immune system cells called hemocytes.…”

Section: Introductionmentioning

confidence: 99%

Antifungal and Anti-Virulent Activity of Origanum majorana L. Essential Oil on Candida albicans and In Vivo Toxicity in the Galleria mellonella Larval Model

et al. 2022

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The aim of this study was to investigate and compare in detail both the antifungal activity in vitro (with planktonic and biofilm-forming cells) and the essential oil composition (EOs) of naturally growing (OMN) and cultivated (OMC) samples of Origanum majorana L. (marjoram). The essential oil composition was analyzed using GC-MS. The major constituent of both EOs was carvacrol: 75.3% and 84%, respectively. Both essential oils showed high antifungal activity against clinically relevant Candida spp. with IC50 and IC90 less than or equal to 0.5 µg mL−1 and inhibition of biofilm with a concentration of 3.5 µg mL−1 or less. Cultivated marjoram oil showed higher anti-biofilm activity against C. albicans. In addition, OMC showed greater inhibition of germ-tube formation (inhibition by 83% in Spider media), the major virulence factor of C. albicans at a concentration of 0.125 µg mL−1. Both EOs modulated cell surface hydrophobicity (CSH), but OMN proved to be more active with a CSH% up to 58.41%. The efficacy of O. majorana EOs was also investigated using Galleria mellonella larvae as a model. It was observed that while the larvae of the control group infected with C. albicans (6.0 × 108 cells) and not receiving treatment died in the controls carried out after 24 h, all larvae in the infected treatment group survived at the end of the 96th hour. When the treatment group and the infected group were evaluated in terms of vital activities, it was found that the difference was statistically significant (p < 0.001). The infection of larvae with C. albicans and the effects of O. majorana EOs on the hemocytes of the model organism and the blastospores of C. albicans were evaluated by light microscopy on slides stained with Giemsa. Cytological examination in the treatment group revealed that C. albicans blastospores were phagocytosed and morphological changes occurred in hemocytes. Our results indicated that the essential oil of both samples showed strong antifungal activities against planktonic and biofilm-forming C. albicans cells and also had an influence on putative virulence factors (germ-tube formation and its length and on CSH).

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Effects of Copper Oxide Nanoparticles on Tissue Accumulation and Antioxidant Enzymes of Galleria mellonella L.

Cited by 49 publications

References 30 publications

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

Impact of Copper Oxide and Selenium Nanoparticles on the Activities of Myeloperoxidase and Gamma-Glutamyl Transferase Related Oxidative Stress of Myocardial Infarction Patients

Antifungal and Anti-Virulent Activity of Origanum majorana L. Essential Oil on Candida albicans and In Vivo Toxicity in the Galleria mellonella Larval Model

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