Effects of corn silage and grass silage in ruminant rations on diurnal changes of microbial populations in the rumen of dairy cows

A Structural and Functional Elucidation of the Rumen Microbiome Influenced by Various Diets and Microenvironments

“…There were no diet, sample fraction or host related shifts in community structure of archaea probably due to the less versatile metabolic capabilities when compared to bacteria (Henderson et al, 2015 ) and the relatively low numbers of identified proteins and OTUs. The findings of the present study support the results of a previous study that analyzed the abundance of total methanogens and the Rumen Cluster C in the same sample material using quantitative real-time PCR (Lengowski et al, 2016 ). However, in contrast to the bacterial datasets, the archaeal community composition inferred from the quantified proteins differed clearly from the structure obtained by Illumina amplicon sequencing (Figure 3 ).…”

“…This may emphasize the benefits and the necessity of applying multiple, complementary methods to investigate the microbiomes of complex ecosystems like the rumen. A previous study analyzed the identical sample material by quantitative real-time PCR and reported no dietary impact on the abundance of S. ruminantium (Lengowski et al, 2016 ). Contrarily, the LFQ-values of proteins of the Selenomonadaceae family assessed in the present study revealed a lower abundance throughout the grass silage-based diet when compared to the corn silage- and the hay-based samples (Figure 2 ) including proteins involved in propionate formation (Table S7 ) and with sequence similarity to SLH (Table S3 ).…”

“…The animals of this study were kept according to the German Animal Welfare legislation at the Agricultural Experiment Station Meiereihof of the University of Hohenheim in Stuttgart, Germany. The experimental procedures and treatments were authorized by the Regierungspräsidium Stuttgart in Germany as previously reported (Lengowski et al, 2016 ).…”

“…Various methods have been employed to study the rumen microbiome ranging from classical cultivation (Bryant, 1959 ; Hungate et al, 1964 ) to molecular approaches including next generation sequencing (Edwards et al, 2004 ; Mackie and Cann, 2005 ; Creevey et al, 2014 ) and functional metagenomics (Brulc et al, 2009 ; Hess et al, 2011 ; Ferrer et al, 2012 ) as well as metabolomics (Ametaj et al, 2010 ; Saleem et al, 2013 ). Furthermore, investigations of diet induced shifts in microbial community composition of the rumen in different contexts are numerous (Tajima et al, 2001 ; Fernando et al, 2010 ; Kong et al, 2010 ; Pitta et al, 2010 ; de Menezes et al, 2011 ; Ann Huws et al, 2012 ; Belanche et al, 2012 ; Carberry et al, 2012 ; Petri et al, 2013 ; Thoetkiattikul et al, 2013 ; Zhang et al, 2013 ; Lengowski et al, 2016 ) but generally rumen studies are restricted to nucleic acids-based approaches with limited functional insights.…”

Antibiofilm Activity of <i>Lactobacillus</i> Strains

“…These consortia are formed by cells of different microbial species [3] consist of microbial cells and a wide range of self-generated extracellular polymeric substances (EPS), including polysaccharides, nucleic acids, and proteins. [2,4]. The biofilms are attached on different, according to their nature and structure substrates, from which the microbial cells derive their nutrients [2,5,6] This form of co-existence of microorganisms ensures the resistance of cells against the effects of high temperatures, osmotic pressure, high salt content and the low pH of the medium [2,7].…”