2022
DOI: 10.7717/peerj.13945
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Effects of CRISPR/Cas9-mediated stearoyl-Coenzyme A desaturase 1 knockout on mouse embryo development and lipid synthesis

Abstract: Background Lipid synthesis is an indispensable process during embryo and growth development. Abnormal lipid synthesis metabolism can cause multiple metabolic diseases including obesity and hyperlipidemia. Stearoyl-Coenzyme A desaturase 1 (SCD1) is responsible for catalyzing the synthesis of monounsaturated fatty acids (MUFA) and plays an essential role in lipid metabolism. The aim of our study was to evaluate the effects of SCD1 on embryo development and lipid synthesis in a knockout mice model.… Show more

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Cited by 5 publications
(2 citation statements)
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“…Following these results, we further designed the sgRNAs based on the sequence of the mouse Scd1 gene in NCBI. The sgRNAs were designed on the CHOPCHOP website ( , accessed on 18 July 2020) to target the first and second Exon region, and 2 high-efficiency sgRNAs were selected [ 32 ] ( Figure 1 C, and Table 1 ) for embryo microinjection ( Figure 1 D and Figure S1A,B ). We first asked whether the concentration of sgRNA/Cas9 had an effect on the gene editing efficiency.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Following these results, we further designed the sgRNAs based on the sequence of the mouse Scd1 gene in NCBI. The sgRNAs were designed on the CHOPCHOP website ( , accessed on 18 July 2020) to target the first and second Exon region, and 2 high-efficiency sgRNAs were selected [ 32 ] ( Figure 1 C, and Table 1 ) for embryo microinjection ( Figure 1 D and Figure S1A,B ). We first asked whether the concentration of sgRNA/Cas9 had an effect on the gene editing efficiency.…”
Section: Resultsmentioning
confidence: 99%
“…We created Scd1 knockout mice based on our previous research [ 32 ]. In addition, 8-week-old C57BL6 female mice and ICR mice were used as embryo donors and recipients.…”
Section: Methodsmentioning
confidence: 99%