Effects of cryopreservation methods in liquid nitrogen on viability of Puccinia abrupta var. partheniicola urediniospores

Host specificity testing and assessment of the pathogenicity of the rust, Puccinia abrupta var. partheniicola, as a biological control agent of Parthenium weed (Parthenium hysterophorus)

“…The rust was imported into Australia in 1991 following studies to assess the most effective method of storing the rust prior to transport (Holden & Smith, 1992). The first field releases were made by the Queensland Department of Lands in June of that year.…”

Conservation of Cerealfungi Following Different Methods of Preservation for Long Terms

“…graminis (Elliott, 2005) and strawberry pathogens (Legard and Chandler, 2000), and the same used 70 fungal isolates in this research kept as a fungal suspension in sterile distilled water at 4°C and by freezing of fungal cultures at -16°C (Sakr, 2018(Sakr, , 2019a. On the other hand, Holden and Smith (1992) described problems of contamination in collections of fungi stored with the continuous subculturing method in PDA due to mites or other causes. Longevity of water-stored cultures may be more or less efficient for an individual species or genus followed by its optimal storage condition.…”

“…Such isolates are also essential for assessing disease resistance and for plant breeding strategies (Abd-Elsalam et al, 2010). Continual sub-culture is the most common method of fungal isolate preservation in isolate collections but is not suitable for prolonged preservation because it is taking a lot time and vulnerable to contamination (Holden and Smith, 1992). Over the years, several methods for the storage of fungal cultures have been assessed in order to reduce theses disadvantages, even though results depend on the fungus group in question.…”