Effects of cryopreservation on meiotic spindles of oocytes and its dynamics after thawing: clinical implications in oocyte freezing—a review article

Chen, Shee‐Uan; Lien, Yih-Ron; Chao, Kuang Han; Ho, Hong Nerng; Yang, Yu Shih; Lee, T.Y.

doi:10.1016/s0303-7207(03)00070-4

Cited by 146 publications

(99 citation statements)

References 48 publications

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“…These changes may trigger tubulin depolymerization and microtubular disassembly with deleterious effects on chromosomal organization [1]. It has been shown that only a small part of the oocytes could completely recover spindle integrity after a few hours of culture at the in vivo temperature [47].…”

Section: Discussionmentioning

confidence: 99%

“…The most efficient way to have oocytes on-call for reproductive applications is to store them under liquid nitrogen [1]. However, the larger volume and smaller surface-to-volume ratio of oocytes makes their cryopreservation more difficult, especially in mammals [2].…”

Section: Introductionmentioning

confidence: 99%

“…Meiotic spindles are dynamic structures consisting of microtubules which concomitantly organize and disorganize during the vitrification procedure [27] and these might be affected by temperature reduction, cryoprotectant concentration and in vitro culture [28]. Disorganization of meiotic spindles might lead to chromosomal dispersion, aneuploidy or polyploidy of in vitro matured cryopreserved oocytes, as well as failure of normal fertilization and development [1]. However, post-thawing incubation could compensate for these problems to some degree, but not completely [29].…”

Section: Introductionmentioning

confidence: 99%

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In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. Methods Freshly isolated (control group) and vitrifiedwarmed COCs (cryotop group) were matured in vitro. The expression of pro-and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively. The expression rates of proand anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p<0.05). Conclusion Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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“…That was mainly due to the low rates of survival, fertilization, and development. Cryopreservation may lead to the premature release of cortical granules, hardening of the zona, and depolymerization of meiotic spindles (1). In recent years, modifications of the slow-freezing method or vitrification have significantly improved the survival rate (2)(3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization

Chen

Lien

Chang

et al. 2004

J Assist Reprod Genet

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Purpose : To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle. Methods : The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed. Results : After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX. Conclusions : In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.

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“…La criopreservación de oocitos bovinos es valiosa al favorecer la disponibilidad de material genético para los procedimientos de producción de embriones, ingeniería genética y preservación de germoplasma (1), sin embargo, el éxito de esta tecnología ha sido deficiente (2), debido a la alta sensibilidad y vulnerabilidad de los oocitos cuando son expuestos a la hipotermia (3, 4), lo que se refleja en las bajas tasas de sobrevivencia, fertilización y desarrollo a partir de oocitos criopreservados (5).…”

Section: Introductionunclassified

EFECTO DE LA MADURACIÓN in vitro DE OOCITOS BOVINOS CON SUERO FETAL BOVINO SOBRE SU ACTIVIDAD MITOCONDRIAL POST-DESVITRIFICACIÓN

Betancur¹,

Vásquez²

2009

Rev MVZ Córdoba

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Objetivo. Evaluar el efecto de la maduración in vitro (MIV) con suero fetal bovino (SFB) sobre la actividad mitocondrial (potencial de membrana) de oocitos bovinos desvitrificados. Materiales y métodos. Se recolectaron oocitos bovinos a partir de ovarios post-mortem, para su posterior MIV en medio Sintetic Oviductal Fluid Amino Acids (SOFaa) en tratamientos con y sin SFB. Posteriormente, fueron destinados para su criopreservación mediante vitrificación en Open Pulled Straw (OPS). Los oocitos fueron desvitrificados y evaluados para su potencial de membrana mitocondrial mediante el uso del colorante fluorescente JC-1. Los datos fueron analizados mediante la prueba t de Student y un análisis de varianza. Resultados. Los resultados evidenciaron una diferencia estadística (p

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Effects of cryopreservation on meiotic spindles of oocytes and its dynamics after thawing: clinical implications in oocyte freezing—a review article

Cited by 146 publications

References 48 publications

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization

EFECTO DE LA MADURACIÓN in vitro DE OOCITOS BOVINOS CON SUERO FETAL BOVINO SOBRE SU ACTIVIDAD MITOCONDRIAL POST-DESVITRIFICACIÓN

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