Effects of Cryptosporidium parvum infection on intestinal fungal microbiota in yaks (Bos grunniens)

Lu, Sijia; Zou, Wen; Chen, Xiushuang; Sun, Guangming; Cidan, Yangji; Almutairi, Mikhlid H.; Dunzhu, Luosang; Nazar, Mudassar; Mehmood, Khalid; Zhu, Yanbin; Basang, Wangdui; Li, Kun

doi:10.1016/j.micpath.2023.106322

Cited by 6 publications

(6 citation statements)

References 38 publications

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“…Pichia and Sporisorium are two major genera in healthy humans [31,38], while higher amounts of Lecanicillium were found in healthy Tibetan pigs compared with diarrheal animals [39]; the higher abundances of these genera in the DZ groups means that concentrated feed #2 increased the abundance of beneficial fungi in the sheep. A higher abundance of Mortierella was detected in healthy yaks compared with ruminants infected with Cryptosporidium parvum [40]. A lower abundance of Plectosphaerella was examined in fluoride-induced mice [41,42], Tomentella was positively related to the generation of short-chain fatty acids [43][44][45] and Filobasidium reduced ulcerative colitis [46]; the higher abundance of this genus in the EZ group revealed that concentrated feed #3 promoted sheep health by mediating fungi abundance.…”

Section: Discussionmentioning

confidence: 96%

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Ren,

Ciwang,

Wang

et al. 2023

Life

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The gut microbiome plays an important role in the metabolism, nutrient absorption and immunocompetency of animals. The dynamics of the microbiota can be influenced by modulatory factors that involve nutrition, environment, health, diseases, etc. Few reports have been documented regarding the effects of different feeds on the fungi microbiome of Suffolk crossed with Tibetan sheep. A total of 30 Suffolk crossed with Tibetan sheep (ST sheep) were selected for the study and randomly divided into five equal groups (n = 6): AZ, BZ, CZ, DZ and EZ. Group AZ was fed with alfalfa and oat grass, whereas group BZ was fed with mixture of concentrated feed, alfalfa and oat grass. Groups CZ, DZ and EZ were fed with concentrated feed #1, #2 and #3, respectively. All experimental animals were fed twice a day for four months, and rectum samples were collected for microbiota analysis. Results revealed that 2,781,461 raw reads and 2,333,239 clean reads were achieved in the ST sheep. When compared with the sheep of groups AZ and BZ (164), the shared amplicon sequence variants (ASVs) between AZ and CZ (109), AZ (113) and DZ (118) as well as AZ along with EZ were fewer. Conspicuous different phyla (8) and genera (56) were examined and compared with free-range sheep in AZ. Genera including Xeromyces, Kazachstania, Cordyceps, Rhodotorula, Pichia, Spor, etc. were found higher in animals in the CZ, DZ and EZ groups. The results of this study provide new insights regarding the effects of different feeds on the fungi microbiome of sheep farmed on the plateau. We concluded that the differences in feed in Suffolk crossed with Tibetan sheep altered their gut microbiota.

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Section: Discussionmentioning

confidence: 96%

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Ren,

Ciwang,

Wang

et al. 2023

Life

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“…Statistics have indicated that diarrhea is the cause of half of the mortality amongst unweaned yaks [26,30]. However, several elements, including a harsh climate, malnutrition, and stress reactions, make controlling diarrhea in yaks very challenging [31]. Extensive studies have shown that the disruption of the intestinal microbiota is a significant factor leading to diarrhea [13,15].…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis Reveals A Gut Microbiota Structure and Function Alteration between Healthy and Diarrheic Juvenile Yaks

Zhao,

Mo,

Kulyar

et al. 2024

Animals

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Diarrhea-induced mortality among juvenile yaks is highly prevalent in the pastoral areas of the Qinghai-Tibet plateau. Although numerous diseases have been linked to the gut microbial community, little is known about how diarrhea affects the gut microbiota in yaks. In this work, we investigated and compared changes in the gut microbiota of juvenile yaks with diarrhea. The results demonstrated a considerable drop in the alpha diversity of the gut microbiota in diarrheic yaks, accompanied by Eysipelatoclostridium, Parabacteroides, and Escherichia-Shigella, which significantly increased during diarrhea. Furthermore, a PICRust analysis verified the elevation of the gut–microbial metabolic pathways in diarrhea groups, including glycine, serine, and threonine metabolism, alanine, aspartate, oxidative phosphorylation, glutamate metabolism, antibiotic biosynthesis, and secondary metabolite biosynthesis. Taken together, our study showed that the harmful bacteria increased, and beneficial bacteria decreased significantly in the gut microbiota of yaks with diarrhea. Moreover, these results also indicated that the dysbiosis of the gut microbiota may be a significant driving factor of diarrhea in yaks.

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“…OMEGA Soil DNA Kit (D5625–01) (Omega Bio-Tek, Norcross, GA, USA) was used to extract the genomic DNA (gDNA) from feces, quality and quantity of DNA was assessed and was stored at −20°C. According to the previous reports, the selected hypervariable regions of bacterial 16S rRNA genes (V3–V4) and fungal ITS genes were amplified by PCR using specific primers with Barcode and high-fidelity DNA polymerase ( Lundberg et al., 2013 ; Wen et al., 2022 ; Lu et al., 2023 ). PCR products were subjected to 2% agarose gel electrophoresis, and DNA fragments were excised and purified by using Quant-iT Pico Green dsDNA Assay Kits.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we utilized high-throughput sequencing (HTS) to investigate the effects of different feeding strategies on the gut microbiota structure and function of yaks in Nimu County. HTS is a molecular biology technique with high sequencing flux, fast detection speed, and low error rate, widely used in the field of microbiology research, and has been widely used in the exploration of gut microorganisms ( Lee et al., 2020 ; Lu et al., 2023 ). The application of HTS in determining genomic sequences has been demonstrated for the first time through genome sequencing of Acinetobacter baumannii .…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of intestinal microbiota composition between free-ranged captive yak populations in Nimu County

Zhu,

Lu,

Cidan

et al. 2024

Front. Cell. Infect. Microbiol.

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The intestinal microbiota assumes a pivotal role in modulating host metabolism, immune responses, overall health, and additional physiological dimensions. The structural and functional characteristics of the intestinal microbiota may cause alterations within the host’s body to a certain extent. The composition of the gut microbiota is associated with environmental factors, dietary habits, and other pertinent conditions. The investigation into the gut microbiota of yaks remained relatively underexplored. An examination of yak gut microbiota holds promise in elucidating the complex relationship between microbial communities and the adaptive responses of the host to its environment. In this study, yak were selected from two distinct environmental conditions: those raised in sheds (NS, n=6) and grazed in Nimu County (NF, n=6). Fecal samples were collected from the yaks and subsequently processed for analysis through 16S rDNA and ITS sequencing methodologies. The results revealed that different feeding styles result in significant differences in the Alpha diversity of fungi in the gut of yaks, while the gut microbiota of captive yaks was relatively conserved. In addition, significant differences appeared in the abundance of microorganisms in different taxa, phylum Verrucomicrobiota was significantly enriched in group NF while Firmicutes was higher in group NS. At the genus level, Akkermansia, Paenibacillus, Roseburia, Dorea, UCG_012, Anaerovorax and Marvinbryantia were enriched in group NF while Desemzia, Olsenella, Kocuria, Ornithinimicrobium and Parvibacter were higher in group NS (P<0.05 or P<0.01). There was a significant difference in the function of gut microbiota between the two groups. The observed variations are likely influenced by differences in feeding methods and environmental conditions both inside and outside the pen. The findings of this investigation offer prospective insights into enhancing the yak breeding and expansion of the yak industry.

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Effects of Cryptosporidium parvum infection on intestinal fungal microbiota in yaks (Bos grunniens)

Cited by 6 publications

References 38 publications

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Metagenomic Analysis Reveals A Gut Microbiota Structure and Function Alteration between Healthy and Diarrheic Juvenile Yaks

Comparative analysis of intestinal microbiota composition between free-ranged captive yak populations in Nimu County

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