Effects of Cu, Zn and their mixtures on bioaccumulation and antioxidant enzyme activities in Galleria mellonella L. (Lepidoptera: Pyralidae)

Abstract

The effects of Cu, Zn and their mixture on bioaccumulation and antioxidant enzyme activities of midgut and fat body of Galleria mellonella larvae were investigated. The application of metals as a mixture showed a synergistic effect and the accumulation levels were increased in both tissues. Zn accumulation increased in midgut and fat body of G. mellonella larvae exposed to metal singly. On the other hand, Cu accumulation increased in midgut, while a decrease was observed in fat body exposed to Cu singly. Moreover, it was determined that oxidative stress was occured in midgut and fat body of G. mellonella larvae with signi cant decreases and increases in antioxidant and detoxi cation enzyme activities when fed singly and in mixture with different concentrations of Cu and Zn. Understanding the reactions of G. mellonella, which is a model organism showing immune system responses similar to vertebrates and bioindicator species, to metals by detoxi cation systems, which is an important parameter in insect physiology, is thought to contribute to future toxicological, genotoxic, physiological and ecotoxicological studies. G. mellonella larvae, which were used as a model organism, shows a similar reaction to mammals.

Exploring the Antioxidant Potential of Talisia esculenta Using In Vitro and In Vivo Approaches

“…The massive influence of CuSO 4 exposure on the larval development and survival rate of Galleria mellonella due to oxidative stress has already been described. For example, it has been verified that CuSO 4 is a source of oxidative stress for invertebrate models [100,101]. Tuncsoy et al [102] also observed a modulation on the antioxidant enzyme activity for G. mellonella larvae exposed to copper oxide nanoparticles.…”