Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Taufiq, Muhammad; Darah, I.

doi:10.22207/jpam.13.4.67

Cited by 4 publications

(7 citation statements)

References 46 publications

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“…The present finding agreed with Salehi et al 45 who revealed the culture medium nourished with Corylus avellana extract could enhanced paclitaxel production of Epicoccum nigrum YEF2 and its growth too. The present results confirm the previous study conducted by Taufiq and Darah 46 .…”

Section: Resultssupporting

confidence: 93%

Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae

Jalil¹,

Zakaria²,

Demirtaş³

2021

J. Pure Appl. Microbiol.

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The effect of incorporating plant extract in the culture medium on anti-MRSA activity of Lasiodiplodia pseudotheobromae IBRL OS-64, was investigated in the present study. On disk diffusion assay, the ethyl acetate fungal extract from culture medium supplemented with host plant extract (HPE) of Ocimum sanctum leaves demonstrated good anti-MRSA activity with a diameter inhibition zone of 22.6±0.6 mm. Meanwhile, the minimal inhibition concentration (MIC) values of the extract from YES broth and YES broth incorporated with HPE were 1000 µg/mL and 250 µg/mL, respectively. The MBC values were 8000 µg/mL and 500 µg/mL, respectively. The YES + HPE extracts exerted bactericidal effect against the test bacteria since the MBC/MIC ratio was less than or equal to 4. The time-kill study revealed a 90% of growth reduction of MRSA ATCC 33591 after 16 h exposure to the fungal extract cultured in YES + HPE. Ironically, for fungal extract grown in YES broth, time-kill curve showed a regrowth pattern of bacterial cells after 24 h exposed to the extract. Therefore, the present study suggested that the addition of HPE in the culture medium could enhance the anti-MRSA activity of endophytic fungus, L. pseudotheobromae IBRL OS-64 against MRSA ATCC 33591.

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Section: Resultssupporting

confidence: 93%

Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae

Jalil¹,

Zakaria²,

Demirtaş³

2021

J. Pure Appl. Microbiol.

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“…Cultural conditions have a significant effect on microbial growth and the production of their secondary metabolites. Taufiq and Darah (2019) reported the improvement of cultural conditions, including pH, temperature, light intensity and the age of seed culture, could significantly enhance the anti-MRSA activity of the endophytic fungus, Lasiodiplodia pseudotheobromae IBRL OS-64. In this study, the physical parameters such as culture duration, pH, temperature, agitation speed and percentage of agar significantly influenced the production of prodigiosin by S. marcescens IBRL USM84.…”

Section: Discussionmentioning

confidence: 99%

“…Anti-MRSA activity was determined according to the method described by Taufiq and Darah (2019). One loop full of MRSA colony at 24 h old was inoculated in a 20.0 mL universal bottle containing 5.0 mL of nutrient broth and incubated at 37 °C for 24 h. The 0.5 McFarland was prepared as a standard for visually adjusting the turbidity of MRSA inoculums.…”

Section: Assay For Anti-mrsa Activitymentioning

confidence: 99%

Effect of physical parameters in enhancing prodigiosin production and anti-MRSA activity of marine bacterium, Serratia marcescens IBRL USM84

Nazari¹,

Ibrahim²,

Zakaria³

et al. 2023

MJM

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Aims: Marine bacteria have been reported to produce potential natural pigment with pharmaceutical properties and their growth can be manipulated in the laboratory to increase pigment production and their antimicrobial activity. Hence, this study aimed to enhance the prodigiosin production in Serratia marcescens IBRL USM84 by improving physical conditions. Methodology and results: The quantification of the pigment produced by S. marcescens IBRL USM84, bacterial cell growth, and its antibacterial activity in the broth medium were determined using a spectrophotometry method. Meanwhile, the antibacterial effect of red pigment on MRSA cells was observed under a scanning electron microscope (SEM). This marine isolate produced the highest yield of prodigiosin (6.95 µg/mL) when cultivated in marine broth with the addition of 0.2% of agar, 25 °C incubation temperature, initial medium pH of 7, 150 rpm of agitation speed for 48 h of cultivation time under light illumination. There was an increment of 151.81% in prodigiosin production after enhancement compared to before the enhancement of cultural conditions. SEM observations revealed that severe damage to the cell's morphologies was exposed to red pigment as indicated by the formation of small dents, which led to completely collapse and eventually, cell death. Conclusion, significance and impact of study:A positive correlation between pigment production and antibacterial activity was observed in the present study. The results supported the fact that marine bacteria are a reservoir of various pigments with antimicrobial properties. Also, the pigment production by S. marcescens and its antibacterial activity were significantly influenced by physical parameters.

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“…The pH of culture medium was adjusted the to 6.0. (10). The flask then was incubated according to the predetermined conditions (120 rpm, 30 0 C, dark condition).…”

Section: Anti-mrsa Potential Profilingmentioning

confidence: 99%

“…The anti-MRSA potential and fungal growth were measured at interval of two days for consecutively 30 days. The steps for this investigation were performed independently three times (10).…”

Section: Anti-mrsa Potential Profilingmentioning

confidence: 99%

Enhancement of Anti-MRSA Potential Produced by an Endophytic Fungus Ceratobasidium Ramicola IBRLCM127 via Submerged Fermentation System

Abdul Rahman,

Abdul Rahim,

Zarkasi

et al. 2023

MJMHS

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Introduction: Exploring endophytic fungi isolated from medicinal herbs could be a turning point in the research of secondary metabolites biosynthesis, as these endophytic fungi are capable of synthesizing the similar compounds as their host plant. The advantages of manipulating endophytic fungi for bioactive compound production are the reduction of dependency rate on slow-growing and rare plants, cost-effective, continuous process, environmentally friendly and high yield in a short period. Thus, the current study envisages investigating the influence of culture conditions against the anti-MRSA potential production of the endophytic fungal isolate, Ceratobasidium ramicola IBRLCM127 isolated from the local medicinal plant Curcuma mangga Valeton & Zijp. Methods: The endophytic fungal isolate was used to produce fungal metabolites through submerged fermentation. The physical parameter improvement was investigated using the ‘one-factor-at-a-time’ technique. The fungal fermentative broth was subjected to an anti-MRSA assay using Lorian method, whereas the growth of a fungus was determined based on the cell growth weight. Results: The highest anti-MRSA potential of 42.50±0.1 U/ml and 5.49±0.1 g/L of mycelial growth was observed after improving the basal medium containing yeast extract sucrose broth incorporated with water extract from the host plant, 6 days old of inoculum age, 2 agar plugs of mycelia, incubation temperature of 25 0C and 12 days of cultivation 12 days of cultivation shaken at 120 rpm in the absence of light. Conclusion: The improved culture conditions shorten the incubation period and yield a significant enhancement of anti-MRSA potential and fungal growth with 13.27% and 10.91%, respectively.

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Effects of Cultural Conditions in Enhancing the Production of Anti-MRSA Activity of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from Leaf of Ocimum sanctum L. in Submerged Fermentation System

Cited by 4 publications

References 46 publications

Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae

Effect of Culture Medium Incorporated with Ocimum sanctum Extract in Enhancing Anti-MRSA activity of Endophytic Fungus, Lasiodiplodia pseudotheobromae

Effect of physical parameters in enhancing prodigiosin production and anti-MRSA activity of marine bacterium, Serratia marcescens IBRL USM84

Enhancement of Anti-MRSA Potential Produced by an Endophytic Fungus Ceratobasidium Ramicola IBRLCM127 via Submerged Fermentation System

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