Effects of cultural conditions on fungal biomass, blastospore yields and toxicity of fungal secreted proteins in batch cultures of <i>Metarhizium anisopliae</i> (Ascomycota: Hypocreales)

Ortiz‐Urquiza, Almudena; Garrido‐Jurado, Inmaculada; Arias-Borrego, Ana; Quesada‐Moraga, Enrique

doi:10.1002/ps.1934

Cited by 16 publications

(8 citation statements)

References 53 publications

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“…The effect of the exposure time on the acute insecticidal activity of the CSPE was studied at a concentration of 1 mg of protein/ml, which was the lethal concentration (LC 99 ) when the CSPE was applied chronically (Ortiz‐Urquiza et al. 2010).…”

Section: Methodsmentioning

confidence: 99%

“…2006a). Previous studies on this CSPE have reported that such insecticide action is due to proteinaceous compounds, among them a monomeric protein of apparent molecular mass of 11 KDa appears as the major responsible for the described effect (Ortiz‐Urquiza et al. 2009, 2010).…”

Section: Introductionmentioning

confidence: 98%

“…Recently, a new insecticide compound, the CSPE from the fermentation product of the EF M. anisopliae (strain EAMa 01/58‐Su), has appeared in the literature as a promising insecticide for the control of the Mediterranean fruit fly, Ceratitis capitata (Wied.) (Diptera: Tephritidae) (Ortiz‐Urquiza et al. 2009, 2010), and other insects (Quesada‐Moraga et al.…”

Section: Introductionmentioning

confidence: 99%

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Insecticidal and sublethal reproductive effects of Metarhizium anisopliae culture supernatant protein extract on the Mediterranean fruit fly

Ortiz‐Urquiza

Vergara-Ortiz

Santiago‐Álvarez

et al. 2010

J Applied Entomology

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Because natural product‐based pesticides are thought to be more rapidly degradable than synthetic chemicals while having a lower ecotoxicological effect, the interest in these products for pest control has grown substantially. Recently, a new insecticide compound, which is the crude soluble protein extract (CSPE) from the fermentation product of the entomopathogenic fungus (EF) Metarhizium anisopliae (strain EAMa 01/58‐Su), has appeared in the literature as a promising insecticide for the control of the Mediterranean fruit fly, Ceratitis capitata. This CSPE has a moderate acute insecticide effect against adults of C. capitata when administered per os at 1 mg of protein/ml, as an exposure time of 8.3 h is necessary to get 50% of mortality. Even though the ingestion of this compound produces serious injuries in the midgut epithelium, it has a very low antifeedant effect on the flies. The effect of sublethal challenges on reproduction of adult flies was studied, and a reduction was found on the female fecundity levels during the first days after the treatment. On the other hand, the sublethal exposure to the CSPE affected neither the egg fertility nor the mortality of the larvae. However, the CSPE did affect the mortality of the pupae from the F1 generation, which suggests that there is a hormetic response from CSPE challenged females. The described sublethal reproductive effects are as important as direct lethal effects in contributing to the total value of the CSPE of the EF M. anisopliae 01/58‐Su strain.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Insecticidal and sublethal reproductive effects of Metarhizium anisopliae culture supernatant protein extract on the Mediterranean fruit fly

Ortiz‐Urquiza

Vergara-Ortiz

Santiago‐Álvarez

et al. 2010

J Applied Entomology

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“…Because of their low mammalian toxicity and residual activity and high host specificity [11], entomopathogenic fungi have been successfully employed against a number of agricultural, urban, and medically important insect pests [12][13][14]. However, the delayed pest mortality by entomopathogenic fungi as compared to synthetic insecticides can be improved either by increasing their toxicity through genetic modifications or by identifying and developing their bioactive metabolites exhibiting lethal and sublethal toxicity to insect pests [15][16][17][18]. Many entomopathogenic fungi have been reported to secrete different antifeedant, insecticidal, and toxic bioactive substances in the broth cultures [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Putative Role of a Yet Uncharacterized Protein Elicitor PeBb1 Derived from Beauveria bassiana ARSEF 2860 Strain against Myzus persicae (Homoptera: Aphididae) in Brassica rapa ssp. pekinensis

et al. 2020

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This study reports the characterization of protein elicitor PeBb1 derived from entomopathogenic fungus Beauveria bassiana ARSEF-2860 strain and its putative role in induced systemic resistance in Brassica rapa ssp. pekinensis against green peach aphid Myzus persicae. The sequence of purified elicitor protein was matched with the genomic sequence of a hypothetical protein BBA_10269 from B. bassiana ARSEF-2860 (GenBank Accession No. XP_008603588.1). The protein-encoding gene PeBb1 contained 534 bp cDNA encoding a polypeptide of 177 amino acids with a molecular mass of 19 kDa. The recombinant elicitor protein was expressed in Escherichia coli using pET-28a (+) expression vector and induced necrosis in the leaves of tobacco. The effects of elicitor protein on aphid M. persicae was determined by applying three different concentrations of PeBb1 (i.e., 26, 35, 53 µM) on B. rapa plants at 4-leaf stage and the treated plants were exposed to newly emerged (0-6 h old) apterous adult aphids. Bioassay results showed significant (p < 0.05) sub-lethal effects of the exogenous application of PeBb1 elicitor on M. persicae. Moreover, the RT-qPCR gene expression analyses showed a significant up-regulation of most of the key genes linked to ethylene (ET)-and jasmonic acid (JA)-associated plant defense pathways in elicitor-treated plants.These results not only recommend the putative utilization of PeBb1 elicitor protein in future biological pest control strategies against phloem-feeding insect pests such as M. persicae, but also help in better comprehension of the mechanisms through which beneficial fungi trigger the induced plant resistance.

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“…know from other entomopathogenic fungi, that a production of 1•5 % dry weight fungal biomass in liquid culture is realistic (Bernhardt, pers. comm., (Ortiz-Urquiza et al 2010). Based on our fluid-bed coating settings and results, 1 L of fermentation culture of JKI-BI-1496 or B.b.007 with 1•5 % dry weight fungal biomass would be enough to produce 25 kg granule with around 100% granule colonization.…”

Section: Accepted Articlementioning

confidence: 94%

Development of a fluid‐bed coating process for soil‐granule‐based formulations of Metarhizium brunneum , Cordyceps fumosorosea or Beauveria bassiana

Stephan

Bernhardt

Buranjadze

et al. 2020

J. Appl. Microbiol.

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Aim: Granule based products of solid state fermented microorganisms are available for biocontrol. Because liquid fermentation has several advantages we investigated fluid-bed coating with liquid fermented biomass. Methods and Results: Biomass containing mycelium or mycelium and submerged spores of the entomopathogenic fungi Metarhizium brunneum, Cordyceps fumosorosea and Beauveria bassiana were produced in liquid culture, separated and different biomass concentrations were adjusted. Based on the examined thermo-tolerance we defined fluid-bed coating This article is protected by copyright. All rights reserved Accepted Article adjustments and investigated granule colonization and sporulation on granules. Granule colonization depended on the biomass concentration and strain. For C. fumosorosea and B. bassiana concentrations of 0•003%dry weight resulted in nearly 100% granule colonization, for M. brunneum with concentrations of 0•7%dry weight in only 50%. The conidiation on granules in sterile soil was highly influenced by the moisture content. Because the granule colonization of M. brunneum was unsatisfactory, we precoated nutrients followed by coating with biomass, submerged spores or conidia. Malt extract had a positive effect on the granule colonization for biomass and submerged spores. Furthermore, aerial conidia can also be coated. Conclusions: Fluid-bed coating of fungal biomass is suitable for the development of granules. Significance and Impact of this Study: With this technology cost efficient biocontrol products can be developed.

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Effects of cultural conditions on fungal biomass, blastospore yields and toxicity of fungal secreted proteins in batch cultures of Metarhizium anisopliae (Ascomycota: Hypocreales)

Abstract: By optimising the culture media, the insecticidal effect of the CSPE against C. capitata can be improved. In the CSPE from G(40):P(20) (40 g L(-1) glucose and 20 g L(-1) peptone in dH(2)O), the LC(50) and the LT(50) were 7 and 4.5 times lower than in the CSPE obtained from Adamek.

Cited by 16 publications

References 53 publications

Insecticidal and sublethal reproductive effects of Metarhizium anisopliae culture supernatant protein extract on the Mediterranean fruit fly

Insecticidal and sublethal reproductive effects of Metarhizium anisopliae culture supernatant protein extract on the Mediterranean fruit fly

Putative Role of a Yet Uncharacterized Protein Elicitor PeBb1 Derived from Beauveria bassiana ARSEF 2860 Strain against Myzus persicae (Homoptera: Aphididae) in Brassica rapa ssp. pekinensis

Development of a fluid‐bed coating process for soil‐granule‐based formulations of Metarhizium brunneum , Cordyceps fumosorosea or Beauveria bassiana

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