Effects of culture conditions on mycelium biomass and intracellular cordycepin production ofCordyceps militaris in natural medium

Xie, Changqing; Liu, Gaixia; Gu, Zhenxin; Fan, Gongjian; Zhang, Lei; Gu, Yan

doi:10.1007/bf03178331

Cited by 17 publications

(8 citation statements)

References 22 publications

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“…The optimal pH for cordycepin production by different strains of C. militaris has been reported to be in the range of 4-7 (Kang et al, 2014;Zhong et al, 2011). Other studies reported that C. militaris strain BCC2816 achieved the highest dry mycelial mass (19.1 g l -l ), and optimal cordycepin yield (1.8 mg l -l ) at 28 °C, pH 6.2 (Chunyan et al, 2009). In general, the effects of pH in static culture of strains AG-1, PSJ-1 in the present study were lower compared with other studies.…”

Section: Effect Of Ph On the Mycelial C Militaris Biomass And Extra-contrasting

confidence: 62%

Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1)

Hung

Wang

Lay

et al. 2020

AAS

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The mycelial biomass, antioxidant activity and production of extra- and intra-cellular polysaccharides production [EPS, IPS] of Cordyceps militaris strains AG-1, PSJ-1 were evaluated under different submerged liquid culture (SLC) conditions. At 24 0C mycelial biomass and polysaccharide production of AG-1, PSJ-1 was optimal using PVC media and static culture conditions; (AG-1: 21.85 ± 1.00; PSJ-1: 18.20 ± 1.84 g l-1 ), and oven drying at 40 oC (AG-1: 25.95 ± 0.84, PSJ-1: 23.55 ± 0.69 mg g-1) compared with hot water extraction (AG-1: 7.07 ± 0.15, PSJ-1: 7.39 ± 0.61 mg g-1). Maximum biomass, EPS and IPS production were observed when the initial pH was 6.7: AG-1: 12.92 ± 0.33, 209.70 ± 1.56, 32.62 ± 0.87; PSJ-1: 9.03 ± 0.24 g l-1 , 198.16 ± 0.85 mg g-1, 30.63 ± 1.96 mg g-1, respectively. The use 3.5 % coconut oil improved biomass, EPS, IPS production, which were 8.27 ± 0.09, 8.01 ± 0.01 g l-1 ; 1208.00 ± 8.60, 1110.40 ± 7.20 mg g-1; 32.43 ± 0.49, 29.74 ± 0.44, for AG-1 and PSJ-1, respectively. Both culture condition and oven drying methods had significant effects on H2O2 and radical scavenging activity, ABTS.+ radical activity, lipid peroxidation, and also had effects on total flavonoid and, total phenolic contents. The use of crude submerged liquid culture and oven drying on strains AG-1, PSJ-1 led to extracts with potent antioxidant activity, suggesting the therapeutic use of polysaccharides from strains AG-1, PSJ-1.

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Section: Effect Of Ph On the Mycelial C Militaris Biomass And Extra-contrasting

confidence: 62%

Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1)

Hung

Wang

Lay

et al. 2020

AAS

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“…In previous work, the orthogonal design method [ 44 – 46 ], Box-Behnken design [ 34 , 47 ], and central composite design [ 31 ] were used to optimize culture conditions for cordycepin production by Cordyceps sp. These experimental designs have been successfully used to optimize medium for the mycelial growth and microbial metabolite production in liquid culture processes.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Large-Scale Culture Conditions for the Production of Cordycepin withCordyceps militarisby Liquid Static Culture

Kang

Wen

Kang

et al. 2014

The Scientific World Journal

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Cordycepin is one of the most important bioactive compounds produced by species of Cordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

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“…Many studies have reported that oxygen supply could evidently influence the formation and accumulation of bioactive metabolites in the submerged cultivation of medicinal fungus [24]. Xie et al [25] also reported that with the increasing of medium capacity dry mycelia weight decreased but cordycepin production enhanced, and obtained the highest cordycepin yield of 1.8 mg g -1 .…”

Section: Nucleoside and Mannitol Analysismentioning

confidence: 99%

Composition and Antitumor Activity of the Mycelia and Fruiting Bodies of Cordyceps militaris

Liu¹,

Huang²,

Zhou³

2014

JFNR

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Cordyceps militaris contains many kinds of bioactive components with the various physiological activities, but has less natural sources of fruiting bodies. Compared with the fruiting bodies from the same origin, the fermented mycelia contained significantly higher amount of protein, crude fat and polysaccharides. The mycelia had more amount of the total amino acids, with proline (3.84 ± 0.12 g 100g -1 ), leucine (2.96 ± 0.10 g 100g -1 ), and aspartic acid (2.68 ± 0.06 g 100g -1 ) as the top three aboundant amino acids, while glutamic acid (3.56 ± 0.07 g 100g - 1), aspartic acid (2.52 ± 0.13 g 100g -1 ), and valine (2.35 ± 0.11 g 100g -1 ) in fruiting bodies. The tested minerals in the mycelia were much more than those in fruiting bodies, especially Selenium which was nondetectable in fruiting bodies. However, the mycelia had lower level of cordycepin and mannitol. The mycelia also exhibited higher antitumor activity against six tested human cancer cell lines, with the range of IC 50 (μg ml -1 ) from 25.03 ± 1.37 to 39.81 ± 0.54. In the animal test for liver cancer cell Hep G2, even high-dose treatment (250 mg kg-1 BW/day) of the extract of the mycelia inhibited the growth of tumor by only 27.6%, significantly lower than the positive control, CTX (30 mg kg -1 BW/day), but had no influence on body weight. The results confirm the potential of cultivated mycelia to be a good substitute of natural products.

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Effects of culture conditions on mycelium biomass and intracellular cordycepin production ofCordyceps militaris in natural medium

Cited by 17 publications

References 22 publications

Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1)

Impact of different fermentation characteristics on the production of mycelial biomass, extra-cellular polysaccharides, intra-cellular polysaccharides, and on the antioxidant activities of Cordyceps militaris (L.) Fr. (strains AG-1, PSJ-1)

Optimization of Large-Scale Culture Conditions for the Production of Cordycepin withCordyceps militarisby Liquid Static Culture

Composition and Antitumor Activity of the Mycelia and Fruiting Bodies of <i>Cordyceps </i><i>m</i><i>ilitaris</i>

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