Effects of culture filtrates of endophytic fungi obtained from Piper aduncum L. on the growth of Mycobacterium tuberculosis

Lima, Alita Moura de; Salem, Júlia Ignez; Souza, João Vicente Braga de; Cortez, Ana Cláudia Alves; Carvalho, Clarice Maia; Chaves, F. C. M.; Veiga, Valdir Florêncio da

doi:10.2225/vol14-issue4-fulltext-11

Cited by 1 publication

(1 citation statement)

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“…Fungal isolates were cultivated on Petri dishes containing potato dextrose agar (PDA) at room temperature for 7-10 days. To obtain secondary metabolites, the fungi were cultured in a broth using a method similar to that described by Lima et al (2011), with some modifications. The bioprocesses were conducted in triplicate.…”

Section: Screening Of Antibacterial Producersmentioning

confidence: 99%

Tapping into Tapajos: antibacterial potential of fungal strains isolated from decaying wood in the Brazilian Amazon

Canto,

Bentes,

Silva

et al. 2023

Braz. J. Biol.

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The emergence of bacterial resistance to antimicrobials poses a significant health threat. To address this issue, exploring the fungal diversity in freshwater environments in the Amazon Forest has potential in the search for new antimicrobials. This study aimed to investigate the production of antibacterial metabolites by aquatic fungi from Amazon lakes, specifically Lake Juá and Lake Maicá (Brazil-PA). The fungal isolates were obtained from wood fragments submerged in these lakes, and the ethyl acetate extracts were evaluated for antibacterial activity against Staphylococcus aureus ATCC 25923, S. aureus (MRSA), ATCC 43300, Escherichia coli ATCC 25922, and E. coli (ESBL) NCTC 13353. Additionally, toxicity of the extracts (EtOAc with antimicrobial activity) against human fibroblasts MRC-5 was investigated. The study identified 40 fungal strains with antimicrobial screening, and the ethyl acetate extracts of Fluviatispora C34, Helicascus C18, Monodictys C15, and Fusarium solani LM6281 exhibited antibacterial activity. F. solani LM6281 showed the lowest minimum inhibitory concentration (MIC) of 50 µg/mL against S. aureus strains and MIC of 100 µg/mL against E. coli strains including ESBL. The cytotoxicity (IC50) of the extract (EtOAc) of F. solani LM6281 was 34.5 µg/mL. Preliminary studies of the TLC culture and RNM-H from the extract (EtOAc) of F. solani suggested the presence of substances from the class of terpenes, quinones, phenolics, and flavonoids. This study highlights the potential of submerged wood fungi in the Amazon region to produce antibacterial substances, thus identifying them as sources of novel bioactive compounds with potential use in the pharmaceutical industry and regional bioeconomy.

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Section: Screening Of Antibacterial Producersmentioning

confidence: 99%