Effects of cultured Cordyceps mycelia polysaccharide A on tumor neurosis factor-α induced hepatocyte injury with mitochondrial abnormality

Tang, Huiling; Wei, Weikun; Wang, Wang; Zha, Zhengqi; Li, Ting; Zhang, Zhijie; Luo, Can; Yin, Huijuan; Huang, Fengjie; Wang, Ying

doi:10.1016/j.carbpol.2017.01.019

Cited by 22 publications

(5 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25 Bcl-2 and Bax proteins as the two major regulators of apoptosis in the Bcl-2 family are functionally opposite. 26,27 Studies have shown tumor cell apoptosis is closely related to the pathway of MAPK. In the researches of AO/EB staining analysis and cell apoptosis analysis, DMDD significantly induced apoptosis of 4T1 cells.…”

Section: Discussionmentioning

confidence: 99%

<p>Anti-Breast Cancer Effect of 2-Dodecyl-6-Methoxycyclohexa-2,5-Diene-1,4-Dione in vivo and in vitro Through MAPK Signaling Pathway</p>

Zhou¹,

Wu²,

Qin³

et al. 2020

DDDT

View full text Add to dashboard Cite

Background: 2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD) has been reported to inhibit a variety of cancer cell lines. The purpose of this study was to investigate the effects of DMDD on 4T1 breast cancer cells and the effects of DMDD on 4T1 breast cancer in mice and its molecular mechanisms. Methods: 4T1 breast cancer cells were treated with different concentrations of DMDD, and their proliferation, apoptosis, cell-cycle distribution, migration, and invasion were detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT, Acridine orange and ethidium bromide dual staining analysis (AO/EB) dual staining, flow cytometry, scratch test, and the Transwell assay. Relative quantitative real-time qPCR analysis and Western blot were applied to examine the expression levels of related genes and proteins. In animal experiments, we established a xenograft model to assess the anti-breast cancer effects of DMDD by evaluating the inhibition rate. The apoptotic activity of DMDD was evaluated by hematoxylin-eosin (HE) staining, transmission electron microscope (TEM) analysis and TdT-mediated dUTP nick end labeling (TUNEL) assays. The mRNA expression levels of MAPK pathway components were detected by relative quantitative real-time qPCR. In addition, the protein expression levels of MAPK pathway components were assessed through immunohistochemical assays and Western blotting. Results: Experiments showed that DMDD could inhibit the proliferation, migration, invasion of 4T1 cells and induce cellular apoptosis and G1 cell cycle arrest. Moreover, DMDD down-regulated the mRNA expressions of raf1, mek1, mek2, erk1, erk2, bcl2, and upregulated the mRNA expression of bax. DMDD reduced the protein expressions of p-raf1, p-mek, perk , p-p38, Bcl2, MMP2, MMP9 and increased the protein expressions of Bax and p-JNK. The results showed that DMDD can effectively reduce the tumor volume and weight of breast cancer in vivo, up-regulate the expression of IL-2, down-regulate the expression of IL-4 and IL-10, induce the apoptosis of breast cancer cells in mice, and regulate the expression of genes and proteins of the MAPK pathway. Conclusion: Our study indicates that DMDD can inhibit proliferation, migration, and invasion and induces apoptosis and cell-cycle arrest of 4T1 breast cancer cells. Also, our findings indicate that DMDD induces the apoptosis of breast cancer cells and inhibits the growth in mice. Its mechanism may be related to the MAPK pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

<p>Anti-Breast Cancer Effect of 2-Dodecyl-6-Methoxycyclohexa-2,5-Diene-1,4-Dione in vivo and in vitro Through MAPK Signaling Pathway</p>

Zhou¹,

Wu²,

Qin³

et al. 2020

DDDT

View full text Add to dashboard Cite

show abstract

“…Overproduced ROS adversely affects biomolecules such as DNA, RNA, proteins, and enzymes [ 40 ]. Mitochondria play a significant role in apoptosis initiation, ROS formation, and energy metabolism [ 41 ]. When malondialdehyde (MDA) and ROS are accumulated in mitochondria by stimuli such as inflammation, stress, and infection and the activity of antioxidant enzymes is inhibited, the balance of the oxidation-antioxidant system is disrupted, and oxidative stress damage occurs [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Citrus unshiu Peel Attenuates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice due to Modulation of the PI3K/Akt Signaling Pathway and MAPK and NF-κB

Lee

Shin

et al. 2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Aim. Citrus unshiu peel has been used to treat various diseases in traditional East Asian medicine including Korea, and many studies have been reported regarding inflammatory diseases including ulcerative colitis (UC). However, the underlying mechanism by which Citrus unshiu peel modulates inflammation in UC remains unclear. Therefore, this study aimed to evaluate the therapeutic effect and underlying mechanism of Citrus unshiu peel water extract (CUP) for UC. Methods. The experiment for UC was conducted with 8-week-old male Balb/c mice. After 1 week of adaptation, acute colitis was induced in all groups except the normal group by 5% DSS dissolved in drinking water for 1 week. Balb/c mice were divided into 5 groups (n = 8/group): control group (Control), distilled water-treated group (DSS), 100 mg/kg sulfasalazine-treated group (SASP), 100 mg/kg CUP-treated group (CUPL), and 200 mg/kg CUP-treated group (CUPH). The efficacy of CUP on UC was evaluated by biochemical analyses such as ROS and MPO in serum and MDA in tissues, and expression of proteins related to inflammation and apoptosis was evaluated through Western blot. Results. As a result of confirming the macroscopic changes and H&E staining in colon tissues to confirm the preventive and therapeutic effects of CU, decrease in colon length and inflammatory lesions were inhibited in the CUP-treated group compared to the DSS group. In addition, as a result of serum ROS and tissue MDA analysis and oxidative stress-related protein analysis, it was significantly decreased in the CUP-administered group compared to the control group. In addition, treatment with CUP not only inactivated MAPK, p-IκBα, and NF-κBp65 by blocking the PI3K/Akt pathway but also significantly reduced the expression of inflammatory cytokines. Conclusion. These results show that CUP not only suppresses oxidative stress in UC but also regulates inflammation-related proteins and apoptotic proteins by regulating the PI3K/Akt signaling pathway, suggesting that it has the potential as a material for developing new natural therapeutic agents for UC.

show abstract

“…This helped to infer that the cell death was not due to necrosis, but due to apoptosis, which were con rmed by the ow cytometry study.The dual AO/EB staining and ow cytometry analysis suggested that ZJW induced apoptosis of MGC-803 cells in a dose-dependent manner.The Bcl-2 family is one of the key factors that affects the ability to regulate apoptosis of the mitochondrial pathway, which is one of the apoptotic pathways [32]. Bcl-2 and Bax proteins, as the two main regulators of apoptosis in the Bcl-2 family, are functionally opposite [33][34]. The western blot experiments showed that ZJW decreased the expression levels of the Bcl2, and increased the expression levels of Bax, thereby inducing apoptosis of MGC-803 cells.The caspase family, a group of protease enzymes (cysteine proteases), exist as the initiator and the executor of apoptosis in the form of inactive zymogens in mammal cells.…”

Section: Discussionmentioning

confidence: 99%

The Anticancer Effect of Zuojinwan on Gastric Cancer Through Multiple Signaling Pathway via miR-107/RUNX1T1 Axis

Rao

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Zuojinwan (ZJW), a famous Chinese medicine formula, has been widely used to treat gastric cancer (GC) which is the third leading cause of cancer-related death around the world.miR-107, a kind of small non-coding RNAs, play a vital role in the occurrence and development of GC. Aim:A network pharmacology-based strategy combined with in vitro and in vivo experiment were employed to investigate the anticancer effect, potential targets, and molecular mechanism of ZJW against GC by miR-107. Methods:Potential targets and signaling pathways of ZJW to treating gastric cancerby miR-107 were determined usingbioinformatics analysis from public databases. miR-107 and its downstream protein expression levels in GC tissues and cell lines were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) or western blot,which were further confirmed by Luciferase reporter assay and Pearson correlation test.The anticancer effect of ZJW in vitro and in vivo were examined by Nude mouse xenograft tumor model and MGC-803 cells. The possible antitumor mechanism of ZJW was studied by using western blot analysis. Results:A total of 33 bioactive ingredients and 147 gene targets of ZJW acting on GC by miR-107 were identified. The miR-107 level was reduced and RUNX1T1 was down-expressed in GC samples.The expression of target was positively correlated with miR-107 in patients with GC.RUNX1T1 was targeted by miR-107.Since overexpression of miR-107 significantly decreased RUNX1T1 expression in MGC-803 cells, and knockdown of miR-107 enhanced RUNX1T1 expression. ZJW inhibited MGC-803 cells proliferation in dose- and time-dependent manner by activating PI3K/AKT/mTOR pathway and arresting the cell cycle in S phase through regulating the expression of Cyclin A, Cyclin B1 and Cyclin E. ZJW suppressed MGC-803 cells migration in dose-dependent manner by increasing E-cadherin expression. ZJW induced MGC-803 cells apoptosis by Fas death receptor and Bcl-2/Bax pathway. Tumor growth was attenuated by ZJW in nude mouse.miR-107 expression was downregulated in ZJW-treated group compared with untreated group, while the protein expression of RUNX1T1 was upregulated. Conclusions: showed significant anti-tumor activity against gastric cancer MGC-803 cells through multiple signaling pathways via miR-107/RUNX1T1 axis both in vitro and in vivo.

show abstract

Effects of cultured Cordyceps mycelia polysaccharide A on tumor neurosis factor-α induced hepatocyte injury with mitochondrial abnormality

Cited by 22 publications

References 43 publications

<p>Anti-Breast Cancer Effect of 2-Dodecyl-6-Methoxycyclohexa-2,5-Diene-1,4-Dione in vivo and in vitro Through MAPK Signaling Pathway</p>

<p>Anti-Breast Cancer Effect of 2-Dodecyl-6-Methoxycyclohexa-2,5-Diene-1,4-Dione in vivo and in vitro Through MAPK Signaling Pathway</p>

Citrus unshiu Peel Attenuates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice due to Modulation of the PI3K/Akt Signaling Pathway and MAPK and NF-κB

The Anticancer Effect of Zuojinwan on Gastric Cancer Through Multiple Signaling Pathway via miR-107/RUNX1T1 Axis

Contact Info

Product

Resources

About