Effects of culturing bovine oocytes either singly or in groups on development to blastocysts

Doherty, Eva; Wade, M.; Hill, Joanne M.; Boland, M.P.

doi:10.1016/s0093-691x(97)00199-4

Cited by 105 publications

(91 citation statements)

References 13 publications

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“…In bovine, in disagreement with our results, some authors observed that blastocysts obtained after culture individually contained a lower number of cells than embryos cultured in large groups (O'Doherty et al, 1997;Larson and Kubisch, 1999). However, other authors did not find a significant decrease on blastocyst cell number of the embryos cultured singly (Goovaerts et al, 2009 and or in reduced groups (Hoelker et al, 2009).…”

Section: Discussioncontrasting

confidence: 99%

“…IVEP efficiency has increased in recent years, although the quality of these embryos remains lower than in embryos produced in vivo (reviewed by Rizos et al, 2008). In particular, when embryos were cultured individually or in reduced groups, in addition to a lower efficiency of embryo production, the obtained embryos usually presented a lower number of cells per blastocyst (Paria and Dey, 1990;O'Doherty et al, 1997; Nagao et al, 2008: 5 v. 25 embryos). Numerous authors have previously indicated, in several mammalian species, that IVEP efficiency is negatively affected when a single or a low number of oocytes/embryos were in vitro cultured in comparison with a higher number of the same (Paria and Dey, 1990;Lane and Gardner, 1992;Carolan et al, 1996;O'Doherty et al, 1997;Fujita et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…In particular, when embryos were cultured individually or in reduced groups, in addition to a lower efficiency of embryo production, the obtained embryos usually presented a lower number of cells per blastocyst (Paria and Dey, 1990;O'Doherty et al, 1997; Nagao et al, 2008: 5 v. 25 embryos). Numerous authors have previously indicated, in several mammalian species, that IVEP efficiency is negatively affected when a single or a low number of oocytes/embryos were in vitro cultured in comparison with a higher number of the same (Paria and Dey, 1990;Lane and Gardner, 1992;Carolan et al, 1996;O'Doherty et al, 1997;Fujita et al, 2006). Moreover, we have observed that post-fertilisation culture, especially the last part of the culture period, seems to be the most important period for bovine embryo development when single and/or low numbers (5 to 10) of embryos were cultured (Salvador et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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“…In animal models, small culture volume as well embryo grouping have been proposed as two different strategies to improve embryo developmental potential [2,4,5,7,8,10,18,19,30] since autocrine and paracrine factors of embryonic origin are likely to mimick in vivo conditions [1,4,5,7,9,13]. In a recent review, Reed and coauthors [7] describe the impact of group versus individual cultures on embryo development.…”

Section: Discussionmentioning

confidence: 99%

Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes

Minasi

Fabozzi

Casciani

et al. 2014

J Assist Reprod Genet

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Purpose The aim of the present randomized, comparative study was to evaluate the effect of reduced culture volumes on sibling human embryo development. Methods Firstly, sibling injected oocytes obtained from 88 out of 165 consenting couples undergoing infertility treatment were cultured either in large (35 μl) or in small drops (15 μl) of culture medium. Secondly, sibling injected oocytes from 77 couples were cultured either in large (35 μl) or in mini drops (7 μl). Embryo quality on day-2 and day-3 and blastocyst formation rate on day-5 were evaluated. Results No statistically significant difference in terms of embryo quality was detected comparing embryos cultured either in large (35 μl) or small (15 μl) drops until blastocyst stage. Similarly, no difference appeared between large (35 μl) or mini (7 μl) drops until day-3, however a significantly higher blastocyst formation rate was observed in mini (7 μl) drops on day-5. Conclusions Reduced culture volume seems not to influence early embryo development but a reduction of medium appears to positively affect blastocyst development. This supports the hypothesis that the pre-implantation embryo produces autocrine factors which exert a positive effect on embryo development when culture is performed in a reduced volume.

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“…However, for commercial purposes such as use in OPU, where it is usually necessary just to keep the immature oocytes from one donor together, a small number of oocytes/embryos can be cultured (approximately three to six oocytes recovered per nonstimulated cow; Rizos et al, 2005;Chaubal et al, 2006). It has been widely reported that in vitro embryo development in bovine and other mammalian species tends to be suppressed in cultures with a single or low number of embryos (in mouse: Paria and Dey, 1990;Canseco et al, 1992;Lane and Gardner, 1992;Kato and Tsunoda, 1994;in bovine: Palma et al, 1992;Ferry et al, 1994;Keefer et al, 1994;Blondin and Sirard, 1995;Carolan et al, 1996;Donnay et al, 1997;O'Doherty et al, 1997;Ward et al, 2000;Pereira et al, 2005;Fujita et al, 2006). It seems that the culture systems developed to culture embryos in groups could be unsuitable and/or incomplete for individual embryo culture, and there must be some kind of limiting factor or condition in single embryo in vitro culture systems which remain still without knowing (Nagao et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

Salvador

Cebrián-Serrano

Salamone

et al. 1970

Span. j. agric. res.

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The aim of this study was to identify the in vitro development stage at which the culture of a single or low number (n = 5 or 10) of oocytes/embryos could impair development in comparison with culture in group (n = 50). In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group (49.4 vs. 83.0%; 0% vs. 37.8%, respectively; p < 0.05). In Experiment 2 and 3, it was observed no effect on embryo development of culturing single or low number of oocytes during maturation and fertilization, respectively. In Experiment 4, it was observed a detrimental effect on blastocyst rate when cultured single or low number of embryos during post-fertilization in vitro culture (2.9; 10.2-10.8; 33.2% in single, low number of embryos (5-10), and controlgrouped, respectively; p < 0.05). In Experiment 5, it was observed that the last part of the culture period (day 3 onwards) seemed to be more affected by the low number of embryos placed in culture. In conclusion, post-fertilization culture, especially on days 3 to 7 after fertilization, seems to be the most important stage for embryo development on single and/or low number (5-10) of embryos culture.Additional key words: single/low embryo culture. ResumenEfecto del número de ovocitos y embriones en la maduración, fertilización y desarrollo embrionario in vitro en vacunoEl objetivo de este trabajo fue identificar la etapa del proceso de producción de embriones in vitro en la cual, el cultivo individualizado o con bajo número de oocitos/embriones (n = 5 ó 10) penaliza al desarrollo embrionario respecto del cultivo en grupo (n = 50). En el Experimento 1, se confirmó que la producción individualizada de embriones in vitro resulta en una menor tasa de división embrionaria y de blastocistos que las obtenidas con el cultivo en grupo (49,4 vs. 83,0%; 0% vs. 37,8%, respectivamente; p < 0,05). En los Experimentos 2 y 3, no se observó efecto sobre el desarrollo embrionario del cultivo individualizado o de bajo número de embriones en las etapas de maduración y fertilización, respectivamente. En el Experimento 4, se observó un efecto negativo sobre la tasa de blastocistos durante el cultivo in vitro post-inseminación cuando se cultivaron los embriones individualmente o con bajo número [2,9; 10,2-10,8; 33,2% en el cultivo individual, con bajo número de embriones (5-10), y grupo control (50), respectivamente; p < 0,05]. En el Experimento 5, se observó que en la última parte del cultivo (día 3 post-inseminación en adelante) es donde más parece que se manifiesta el efecto negativo del cultivo con número reducido de embriones. Como conclusión, el cultivo post-inseminación, especialmente entre los días 3 y 7 de cultivo, parece ser la etapa más determinante del proceso de producción de embriones in vitro para el desarrollo embrionario en el cultivo individualizado y/o en número reducido de embriones (5-10).Palabras clave adicionales: cultivo individualizado/bajo número de embriones.

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Effects of culturing bovine oocytes either singly or in groups on development to blastocysts

Cited by 105 publications

References 13 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes

Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

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