Effects of cylindrospermopsin, its decomposition products, and anatoxin-a on human keratinocytes

Adamski, Michał; Zimoląg, Eliza; Kamiński, Ariel; Drukała, Justyna; Białçzyk, Jan

doi:10.1016/j.scitotenv.2020.142670

Cited by 10 publications

(6 citation statements)

References 40 publications

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“…Thus, the suggested sensor can be applied for the low-concentration detection of small molecules, while adjusting for the flatness of the gold film needs to be performed to improve the dynamic range. Cyanotoxins are small molecules with low molecular weight, e.g., for CYN this is 415 Da [26], that can hardly be detected without signal amplification. The enhancement of the sensitivity of SPR-based biosensors is discussed in terms of the synergetic effect of graphene resulting in charge transfer from graphene to gold film and the increased adsorption efficiency [27,28].…”

Section: Resultsmentioning

confidence: 99%

“…This provides the Gr/SPR with sufficient sensitivity (Table 1), but still with a more significant level of noise that decreases the dynamic range. Cyanotoxins are small molecules with low molecular weight, e.g., for CYN this is 415 Da [26], that can hardly be detected without signal amplification. The enhancement of the sensitivity of SPR-based biosensors is discussed in terms of the synergetic effect of graphene resulting in charge transfer from graphene to gold film and the increased adsorption efficiency [27,28].…”

Section: Resultsmentioning

confidence: 99%

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Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Jaric

Bajaj

Vukić

et al. 2023

Toxins

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In this work, we report a novel method for the label-free detection of cyanotoxin molecules based on a direct assay utilizing a graphene-modified surface plasmon resonance (SPR) aptasensor. Molecular dynamic simulation of the aptamer’s interaction with cylindrospermopsin (CYN) reveals the strongest binding sites between C18–C26 pairs. To modify the SPR sensor, the wet transfer method of CVD monolayer graphene was used. For the first time, we report the use of graphene functionalized by an aptamer as a bioreceptor in conjunction with SPR for the detection of CYN. In a direct assay with an anti-CYN aptamer, we demonstrated a noticeable change in the optical signal in response to the concentrations far below the maximum tolerable level of 1 µg/L and high specificity.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Jaric

Bajaj

Vukić

et al. 2023

Toxins

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“…CYN is the most frequently studied hepatotoxin after Microcystin so far and their distribution is increasing day by day, they are known to be an effective cytotoxin that affects different organs and their functions in animals, plants causing fatal deaths in cattle and outbreaks in human health situations by causing the illness 49 . In vitro, their decomposed products are toxic to humans and their presence of harmful characters in CYN is huge and primarily it includes the cytotoxicity 55 . It inhibits the glutathione, protein synthesis, and cytochrome P450, also characterized by a tricyclic carbon skeleton attached to the hydroxymethyl uracil, a sulfate group, Guanidine 15,54 .…”

Section: Cylindrospermopsin (Cyn)mentioning

confidence: 99%

“…49 . The main harmful effect after exposure to CYN is the inhibition of protein synthesis due to reaction with eukaryotic translation protein synthesis 55 . Their biological activity involves the interruption of various metabolic pathways and also causes oxidative stress by increasing the concentration of free oxygen radicals 54 .…”

Section: Cylindrospermopsin (Cyn)mentioning

confidence: 99%

Insight into the Potential Cyanobacterial Metabolites and their Screening Strategies

Pooja

Niveshika

2022

Biosci., Biotech. Res. Asia

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Cyanobacteria are the first oxygenic photosynthesis performing prokaryotes. They are considered to have fast growth, amenability to genetic modifications towards photo-autotrophy. Have the ability to grow under heterotrophic conditions with minimum available sunlight to obtain energy by utilizing organic carbon as its substrate. Cyanobacteria are diversely spread in marine, freshwater, terrestrial habitats which differ from each other concerning their structural and functional metabolism. It produces bioactive compounds which are toxic to animals as well as humans which are produced in freshwater habitats whereas marine species of cyanobacteria produce secondary metabolites which are involved in the production of new drugs and also show potential in various fields such as Biotechnological applications, pharmacology, agriculture sustainability, and environmental remediation. Cyanobacteria also produce non-toxic compounds which help in protecting plants by producing phytohormones, siderophores, and UV protective or absorbing compounds. Marine cyanobacterial bioactive compounds are involved in several bioactivities such as antiviral, antialgal, antiprotozoal, antifungal activities, etc. Freshwater species are involved in forming harmful cyanobacterial blooms which are highly toxic to animals as well as humans (Ex: cyanotoxins, hepatotoxins, etc). Different strategies are used to detect the cyanobacterial compounds under in-vivo and in-vitro cultures. To analyze the quality and safety of water, screening methods are necessary to detect possible toxic compounds present in the environmental habitats. Screening methods include microscopy assay, physiological methods, chemical methods, biochemical-based methods, and molecular-based methods. All these methods of screening help in characterizing, identifying the cyanobacterial toxins and also have a few limitations in their reliability, sensitivity, and limit in the detection of the compounds.

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“…Certain secondary metabolites synthesized by cyanobacteria, known as cyanotoxins, are some of the most dangerous compounds produced in nature. Based on their effect on vertebrates, these compounds can be divided into four groups: hepatotoxins, neurotoxins, cytotoxins and dermatotoxins [1][2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Effect of Microcystin-LR, Nodularin, Anatoxin-a, β-N-Methylamino-L-Alanine and Domoic Acid on Antioxidant Properties of Glutathione

Adamski

Kamiński

2022

Life

Self Cite

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Cyanobacteria produce a range of toxic secondary metabolites that affect many processes in human, animal and also plant cells. In recent years, some efforts have concentrated on deepening the understanding of their effect on living cells in the context of the disruption of antioxidant systems. Many results suggest that cyanotoxins interfere with glutathione (GSH) metabolism, which often leads to oxidative stress and, in many cases, cell death. Knowledge about the influence of cyanotoxins on enzymes involved in GSH synthesis or during its antioxidant action is relatively broad. However, to date, there is no information about the antioxidant properties of GSH after its direct interaction with cyanotoxins. In this paper, we investigated the effect of four cyanotoxins belonging to the groups of hepatotoxins (microcystin-LR and nodularin) or neurotoxins (anatoxin-a and β-N-methylamino-L-alanine) on the in vitro antioxidant properties of GSH. Moreover, the same study was performed for domoic acid (DA) produced by some diatoms. The obtained results showed that none of the studied compounds had an effect on GSH antioxidant potential. The results presented in this paper are, to the best of our knowledge, the first description of the kinetics of scavenging radicals by GSH reactions under the influence of these cyanotoxins and DA. This work provides new and valuable data that broadens the knowledge of the impact of cyanotoxins and DA on GSH metabolism and complements currently available information. Future studies should focus on the effects of the studied compounds on antioxidant systems in vivo.

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Effects of cylindrospermopsin, its decomposition products, and anatoxin-a on human keratinocytes

Cited by 10 publications

References 40 publications

Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Label-Free Direct Detection of Cylindrospermopsin via Graphene-Enhanced Surface Plasmon Resonance Aptasensor

Insight into the Potential Cyanobacterial Metabolites and their Screening Strategies

Effect of Microcystin-LR, Nodularin, Anatoxin-a, β-N-Methylamino-L-Alanine and Domoic Acid on Antioxidant Properties of Glutathione

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