2013
DOI: 10.1074/jbc.m112.419689
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Effects of Cysteine Proteases on the Structural and Mechanical Properties of Collagen Fibers

Abstract: Background: Collagen macromolecules are biologically relevant substrates in tissue remodeling and bone-related diseases. Results: We investigated the action of cysteine proteases on the structural integrity and mechanical functionality of collagen fibers. Conclusion: Using ultrastructural and biochemical techniques, we present a model of collagen fiber degradation via cathepsin K. Significance: Our data provide new insights in matrix degradation and may allow new strategies to inhibit it.

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Cited by 86 publications
(83 citation statements)
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“…Cathepsin-K, a collagenase secreted by osteoclasts, cleaves proteoglycans from the fibril exterior before degrading the primary fibril structure. 18 OVX results in increased osteoclast activity coupled to increased cathepsin-K activity. This is directly countered by the ERT therapy resulting in partial prevention of the collagen fibril microstructure changes.…”
Section: Resultsmentioning
confidence: 99%
“…Cathepsin-K, a collagenase secreted by osteoclasts, cleaves proteoglycans from the fibril exterior before degrading the primary fibril structure. 18 OVX results in increased osteoclast activity coupled to increased cathepsin-K activity. This is directly countered by the ERT therapy resulting in partial prevention of the collagen fibril microstructure changes.…”
Section: Resultsmentioning
confidence: 99%
“…Because of the hydrolysis of proteoglycans and telopeptides by myroicolsin, the complex hierarchical structure of collagen fiber was destroyed, and free collagen monomers were produced. Recently, the cysteine protease cathepsin K was reported to disassociate proteoglycans from collagen fiber prior to collagen degradation (53). The cleavage pattern of bovine type I collagen by myroicolsin was further analyzed.…”
Section: Discussionmentioning
confidence: 99%
“…The presence of glycosaminoglycans allows access to the triple helix, leading to the cutting of the fibril into smaller sub-fibrils and a simultaneous release of glycosaminoglycans. This process eventually results in a progressive unfolding of the fibrils, making them less stable and therefore accessible for further degradation [96]. Cathepsins that do not exhibit collagenase activity, such as cathepsins L, V, S, and B have only a limited effect on the fibril structures [96].…”
Section: Cathepsin Kmentioning
confidence: 99%
“…This process eventually results in a progressive unfolding of the fibrils, making them less stable and therefore accessible for further degradation [96]. Cathepsins that do not exhibit collagenase activity, such as cathepsins L, V, S, and B have only a limited effect on the fibril structures [96]. In addition to bone, cathepsin K is also expressed in hematopoietic, epithelial, and fibroblast cells, and was shown to play a role in arthritis, obesity, schizophrenia, bone metastases, and various other pathological conditions [89].…”
Section: Cathepsin Kmentioning
confidence: 99%