Effects of Cytochrome c on Liver Functions of Old Rats

Ohnishi, Haruo; Tsukuda, Shigeru; Hayashi, Yutaka; Ogawa, Norio; Yajima, Gonpachi; Masugi, Yohei; Aihara, Kaoru; Suzuki, Katsuya

doi:10.1038/newbio239084a0

Cited by 8 publications

(6 citation statements)

References 9 publications

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“…David (1985) reported a sevenfold decline in the volume of the average rat liver mitochondrion, whereas Herbener (1976) did not detect any change in this parameter in the mouse liver as a function of aging. A number of studies reported a decrease in the number of mitochondria per volume of liver tissue (Herbener, 1976;Ohnishi et al, 1972;Shamoto, 1968;Tate and Herbener, 1976;Tauchi et al, 1974), an increase (Pieri et al, 1975) and a decrease (David, 1985) in the number per hepatocyte, a 15-20% decline (Pieri et al, 1975;David, 19851, and no change (Meihuizen and Blansjaar, 1980) in the relative volume of the mitochondrial compartment in rat liver tissue during aging ( Fig. 10).…”

Section: Mitochondriamentioning

confidence: 97%

Hepatocyte fine structure during maturation and senescence

Schmucker

1990

J. Elec. Microsc. Tech.

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Aging is accompanied by a myriad of changes in cell structure, function, and composition. The fact that much of the information concerning age-related alterations in cellular morphology is qualitative precludes meaningful correlations with biochemical changes in order to enhance data interpretation. The mammalian liver has been subjected to both qualitative and quantitative evaluations of hepatocyte structure as a function of aging, i.e., development, maturation, and senescence. Although these data are characterized by considerable variability and, in some instances, blatant contradictions, there exists sufficient agreement in several parameters to permit a consensus in the inbred rat model. Certainly the volume of individual hepatocytes increases with age, and many of the organelle compartments reflect this change. While old rats exhibit a high incidence of polyploidy, there is no definitive evidence to demonstrate a concomitant increase in the binuclear hepatocyte index. Several specific hepatocellular organelles undergo changes in their relative volume or surface area that appear to correlate with functional alterations. The volume density of the lysosomal compartment enlarges significantly during senescence and is accompanied by increased activities of several constituent hydrolases. The hepatic concentration of smooth-surfaced endoplasmic reticulum declines markedly with aging, as does the yield of liver microsomes and the activities of several microsomal enzymes, e.g., mono-oxygenases and glucose-6-phosphatase. However, the responses of the majority of hepatocyte organelles to aging is varied and inconsistent based on the limited data currently available.

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Section: Mitochondriamentioning

confidence: 97%

Hepatocyte fine structure during maturation and senescence

Schmucker

1990

J. Elec. Microsc. Tech.

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“…Results of studies on actions of cytochrome c on the liver have been reported by the authors (1)(2)(3)(4). In a previous report (4), a conclusion was reached that the improving action exhibited by cytochrome c on spontaneously decreasing liver function in aged rats results from the improvement of mitochondrial function and an increase in hepatic glutathione.…”

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confidence: 75%

Effects of Cytochrome C on Lipid and Sugar Metabolism in Aged Rats

Ohnishi

Tsukuda

Ogawa

1974

Japanese Journal of Pharmacology

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Results of studies on actions of cytochrome c on the liver have been reported by the authors (1-4). In a previous report (4), a conclusion was reached that the improving action exhibited by cytochrome c on spontaneously decreasing liver function in aged rats results from the improvement of mitochondrial function and an increase in hepatic glutathione.In the present study, attempts have been made to determine the effects of aging on lipid and sugar metabolism as well as the effects of cytochrome c administration of lipid and sugar metabolism in aged rats showing spontaneous decrease in liver function. MATERIALS AND METHODSThe experimental materials and methods conformed to those described in the previ ous report (4). Equine cytochrome c was given to 500 day-old rats i.nn. into thigh or p.o.in the form of enteric-coated granules in a dose of 100 mg/kg for 21 consecutive days and biochemical studies were conducted in comparison with 70 and 250 day-old rats.Method of Lipid Determination: Total serum lipids, serum cholesterol and phospho lipids were measured by means of the Bragdon's oxidation method (5), the Zurkowsky's method (6) and the Hoeflmayer and Fried's method (7) respectively, whereas free fatty acid, triglyceride and f3-lipoprotein were measured by means of the Doncomb's method (8), the Van Handel's method (9) and the heparin calcium chloride sedimentaion method (10) respectively. In determining hepatic lipids, total lipids, cholesterol, phospholipids and neutral fat were measured by the same method as used in serum determinations by extracting lipids from a homogenate prepared by adding 9 ml. of 0.9 % aqueous sodium chloride to 1 g. of the liver with the use of a mixture of chloroform and methanol (2: 1).Analysis of Fatty Acid Composition: The fatty acid composition of the serum total lipids and the hepatic total lipids were analyzed by Higasa's method (11) and Takenaka's method (12), respectively.

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“…It was also reported that the heme protein improves spontaneously, lowered functions of the liver and the lipid metabolism in aged rats (2).…”

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confidence: 99%