Effects of Cytochrome C on the Liver Function of Aged Rats

Ohnishi, Haruo; Tsukuda, Shigeru; Ogawa, Nobuhisa

doi:10.1254/jjp.24.15

Cited by 4 publications

(4 citation statements)

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“…Although the mechanism of the increase in the level of hepatic ATP has yet to be clarified, the increase is attributed to an increase in he patic glutathione and decline in lipid peroxides as described in a previous paper (4). Fur thermore an increase in coenzyme A and a decline in pyruvic acid suggest an increase in the activity of the TCA cycle.…”

Section: Effects Of Cytochronre C Administrationmentioning

confidence: 80%

“…The increase in the level of hepatic ATP suggests improved energy metabolism, coin ciding with an increase in the levels of cytochromes a, b and c by the mitochondria and the reactivation of the tissue respiration (4). Although the mechanism of the increase in the level of hepatic ATP has yet to be clarified, the increase is attributed to an increase in he patic glutathione and decline in lipid peroxides as described in a previous paper (4).…”

Section: Effects Of Cytochronre C Administrationmentioning

confidence: 81%

“…Results of studies on actions of cytochrome c on the liver have been reported by the authors (1)(2)(3)(4). In a previous report (4), a conclusion was reached that the improving action exhibited by cytochrome c on spontaneously decreasing liver function in aged rats results from the improvement of mitochondrial function and an increase in hepatic glutathione.…”

mentioning

confidence: 82%

“…The experimental materials and methods conformed to those described in the previ ous report (4). Equine cytochrome c was given to 500 day-old rats i.nn.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Cytochrome C on Lipid and Sugar Metabolism in Aged Rats

Ohnishi

Tsukuda

Ogawa

1974

Japanese Journal of Pharmacology

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Results of studies on actions of cytochrome c on the liver have been reported by the authors (1-4). In a previous report (4), a conclusion was reached that the improving action exhibited by cytochrome c on spontaneously decreasing liver function in aged rats results from the improvement of mitochondrial function and an increase in hepatic glutathione.In the present study, attempts have been made to determine the effects of aging on lipid and sugar metabolism as well as the effects of cytochrome c administration of lipid and sugar metabolism in aged rats showing spontaneous decrease in liver function. MATERIALS AND METHODSThe experimental materials and methods conformed to those described in the previ ous report (4). Equine cytochrome c was given to 500 day-old rats i.nn. into thigh or p.o.in the form of enteric-coated granules in a dose of 100 mg/kg for 21 consecutive days and biochemical studies were conducted in comparison with 70 and 250 day-old rats.Method of Lipid Determination: Total serum lipids, serum cholesterol and phospho lipids were measured by means of the Bragdon's oxidation method (5), the Zurkowsky's method (6) and the Hoeflmayer and Fried's method (7) respectively, whereas free fatty acid, triglyceride and f3-lipoprotein were measured by means of the Doncomb's method (8), the Van Handel's method (9) and the heparin calcium chloride sedimentaion method (10) respectively. In determining hepatic lipids, total lipids, cholesterol, phospholipids and neutral fat were measured by the same method as used in serum determinations by extracting lipids from a homogenate prepared by adding 9 ml. of 0.9 % aqueous sodium chloride to 1 g. of the liver with the use of a mixture of chloroform and methanol (2: 1).Analysis of Fatty Acid Composition: The fatty acid composition of the serum total lipids and the hepatic total lipids were analyzed by Higasa's method (11) and Takenaka's method (12), respectively.

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Section: Effects Of Cytochronre C Administrationmentioning

confidence: 80%

Section: Effects Of Cytochronre C Administrationmentioning

confidence: 81%

mentioning

confidence: 82%

“…The experimental materials and methods conformed to those described in the previ ous report (4). Equine cytochrome c was given to 500 day-old rats i.nn.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations