Effects of Dangguibuxue decoction on rat glomerular mesangial cells cultured under high glucose conditions

Ren, Xiaojie; Zhang, Ying‐Wen; Wang, Xiuping; Li, Yarong

doi:10.1186/s12906-017-1774-4

Cited by 12 publications

(8 citation statements)

References 39 publications

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“…One study manifested that it could inhibit high glucose-induced GMC proliferation and synthesis of various ECM proteins, indicating the value for treatment of DKD [85]. Other studies also showed that it could inhibit the proliferation of GMCs by attenuating the α -SMA expression and reducing hydroxyproline secretion in cultured GMCs under high glucose conditions [86].…”

Section: The Action Mechanisms Of Chm On Dkdmentioning

confidence: 99%

The Efficacy and Mechanism of Chinese Herbal Medicine on Diabetic Kidney Disease

Zhong

Liu

et al. 2019

Journal of Diabetes Research

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Diabetic kidney disease (DKD) is the most common microvascular complication of diabetes and is one of the main causes of end-stage renal disease (ESRD) in many countries. The pathological features of DKD are the hypertrophy of mesangial cells, apoptosis of podocytes, glomerular basement membrane (GBM) thickening, accumulation of extracellular matrix (ECM), glomerular sclerosis, and tubulointerstitial fibrosis. The etiology of DKD is very complicated and many factors are involved, such as genetic factors, hyperglycemia, hypertension, hyperlipidemia, abnormalities of renal hemodynamics, and metabolism of vasoactive substances. Although some achievements have been made in the exploration of the pathogenesis of DKD, the currently available clinical treatment methods are still not completely effective in preventing the progress of DKD to ESRD. CHM composed of natural products has traditionally been used for symptom relief, which may offer new insights into therapeutic development of DKD. We will summarize the progress of Chinese herbal medicine (CHM) in the treatment of DKD from two aspects. In clinical trials, the Chinese herbal formulas were efficacy and safety confirmed by the randomized controlled trials. In terms of experimental research, studies provided evidence for the efficacy of CHM from the perspectives of balancing metabolic disorders, reducing inflammatory response and oxidative stress, antifibrosis, protecting renal innate cells, and regulating microRNA and metabolism. CHM consisting of different ingredients may play a role in synergistic interactions and multiple target points in the treatment of DKD.

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Section: The Action Mechanisms Of Chm On Dkdmentioning

confidence: 99%

The Efficacy and Mechanism of Chinese Herbal Medicine on Diabetic Kidney Disease

Zhong

Liu

et al. 2019

Journal of Diabetes Research

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“…It was found that the AM-AS compound could affect the concentration of TNF-α 28 , and their extracts inhibit the proliferation and fibrosis of glomerular mesangial cells (GMCs) in diabetic rats 49 . AM polysaccharides can reduce the levels of serum IL-6 and AngII in DM rats, and inhibit the expression of TGF-β1 and TNF-α 50 .…”

Section: Discussionmentioning

confidence: 93%

Network pharmacology-based investigation of potential targets of astragalus membranaceous-angelica sinensis compound acting on diabetic nephropathy

Dong

Zhao

Wang

2021

Sci Rep

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To explore the mechanism of the Astragalus membranaceous (AM)-Angelica sinensis (AS) compound in the treatment of diabetic nephropathy (DN) we used network pharmacology and molecular docking. Screen the components and targets of the AM-AS compound in the TCMSP and the BATMAN-TCM, and establish a component-target interaction network by Cytoscape 3.7.2. After searching relevant targets of DN in related databases, the common targets of the AM-AS compound and DN were obtained by comparison. Gene ontology (GO) analysis and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway enrichment analysis were performed through David database. Molecular docking was performed by PyMoL2.3.0 and AutoDock Vina software. After screening, 142 main targets of the AM-AS compound in the treatment of DN have been identified. Target network was established and the topology of PPI network was analyzed. KEGG pathway enrichment analysis shows that these targets are related to apoptosis, oxidative stress, inflammation, insulin resistance, etc. Molecular docking shows that the target proteins have good combinations with the main active components of the AM-AS compound. AM-AS compound may treat DN by acting on VEGFA, TP53, IL-6, TNF, MARK1, etc., and regulate apoptosis, oxidative stress, inflammation, glucose, and lipid metabolism processes. The in vivo study results suggest that AM-AS compound can significantly reduce the FBG level of diabetic rats, increase the level of INS, improve renal functions, reduce urinary proteins, inhibit glycogen deposition, granulocyte infiltration and collagen fiber proliferation in renal tissue, and restrain the progress of DN. In vivo study combined with network pharmacology and molecular docking methods provides new ideas for the pathogenesis and treatments of DN.

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“…ECM protein deposition, including Collagen and Fibronectin, was related to the development of renal glomerular fibrosis in DN [29]. Besides, HG treatment induced ECM deposition, inflammation, and oxidative stress in HGMC cells, which were key pathological features of multiple renal diseases and widely reported in previous studies [30,31]. To investigate the biological role of KCNQ1OT1 in DN, HGMC cells treated with 30 mM glucose were used as the cellular model for DN.…”

Section: Discussionmentioning

confidence: 94%

lncRNA KCNQ1OT1 regulated high glucose-induced proliferation, oxidative stress, extracellular matrix accumulation, and inflammation by miR-147a/SOX6 in diabetic nephropathy (DN)

Zhan

2022

Endocr J

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Long non-coding RNAs (lncRNAs) have been proved to play critical roles in diabetic nephropathy (DN). This study aimed to investigate the functions and underlying mechanism of potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (KCNQ1OT1) in DN. Blood samples were obtained from 33 DN patients and 30 healthy volunteers. Kidney biopsies tissues of DN patients (n = 10) and patients with normal kidney morphology (n = 10) were collected. We found that KCNQ1OT1 was markedly overexpressed in the blood and kidney biopsies tissues of DN patients, as well as in high glucose (HG)-cultured human glomerular mesangial (HGMC) cells. Knockdown of KCNQ1OT1 suppressed proliferation, extracellular matrix (ECM) accumulation, inflammation, and oxidative stress in HG-treated HGMC cells in vitro. KCNQ1OT1 functioned as a sponge for microRNA-147a (miR-147a), and SRY-Box Transcription Factor 6 (SOX6) was directly targeted by miR-147a. Downregulation of miR-147a or upregulation of SOX6 partly overturned the prohibitive effects of KCNQ1OT1 knockdown or miR-147a overexpression on proliferation, ECM accumulation, inflammation, and oxidative stress in HG-treated HGMC cells. Altogether, KCNQ1OT1 mediated the proliferation, ECM accumulation, inflammation, and oxidative stress in HG-treated HGMC cells via miR-147a/SOX6 axis, which might be a novel target for DN therapy.

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Effects of Dangguibuxue decoction on rat glomerular mesangial cells cultured under high glucose conditions

Cited by 12 publications

References 39 publications

The Efficacy and Mechanism of Chinese Herbal Medicine on Diabetic Kidney Disease

The Efficacy and Mechanism of Chinese Herbal Medicine on Diabetic Kidney Disease

Network pharmacology-based investigation of potential targets of astragalus membranaceous-angelica sinensis compound acting on diabetic nephropathy

lncRNA KCNQ1OT1 regulated high glucose-induced proliferation, oxidative stress, extracellular matrix accumulation, and inflammation by miR-147a/SOX6 in diabetic nephropathy (DN)

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