Effects of Dangkwisoo-san, a traditional herbal medicine for treating pain and blood stagnation, on the pacemaker activities of cultured interstitial cells of Cajal

Sung, Soon Ki; Kim, Sung Jin; Ahn, Tae-Seok; Hong, Noo Ri; Park, Hyun Soo; Kwon, Young Kyu; Kim, Byung Joo

doi:10.3892/mmr.2015.4203

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…This study suggested the functional interaction between Qui and β3-adrenoceptors, although it warrants further investigation. Recent studies on herbal medicines, such as Lizhong Tang and Dangkwisoo-san, reported that these modify the gastrointestinal motility or pacemaker activities of ICCs [30,31]. In addition, some herbal extracts of the roots of ginger and Liriope Platyphylla Wang et Tang have been reported to mediate the contraction of the ileal smooth muscle in rats [32,33].…”

Section: Discussionmentioning

confidence: 99%

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine

Yamashita¹,

Shimamoto²,

Morita³

et al. 2018

JFNS

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Previously, the effects of several bitter substances have been investigated in the contraction of the murine jejunum and ileum, reporting that these independently suppress the rhythm generation of the interstitial cells of Cajal. Recently, it was reported that thiamin, which binds to a bitter taste receptor, modifies the early phase of the ileum contraction, whereas the physiological effects on the rhythm and amplitude of jejunum and ileum contractions remain unclear. In this study, it was investigated the physiological effects of thiamin and quinine on the in vitro contraction of the murine jejunum and ileum using mice for all experiments. the periodic contraction of the jejunum was observed before the administration of acetylcholine (Ach) and other substances, and the tonic amplitudes induced by the substances. These bitter substances variably affect the Achinduced rhythmic contraction of the jejunum and ileum in vitro. In addition, quinine hydrochloride (Qui) and thiamin hydrochloride (Thi) variably affect the Ach-induced rhythmic contraction of the jejunum and ileum in vitro. Both Qui and Thi markedly increase the rhythmic contraction in the jejunum. Although Thi does not change the rhythmic contraction in the ileum, it gradually reduces the amplitude in the jejunum. Conversely, Qui gradually reduces the amplitude and almost inhibits the contraction in the jejunum. Furthermore, an antagonist of the adrenalin-beta3 receptor, SR59230A, enhances the Quiinduced inhibition of the contraction in the jejunum.

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Section: Discussionmentioning

confidence: 99%

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine

Yamashita¹,

Shimamoto²,

Morita³

et al. 2018

JFNS

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“…ese results have been obtained mainly from cell-based experiments with ICCs using the patch-clamp technique. Many single-herb extracts [41,42], TCM drugs [14,26,[43][44][45][46][47][48][49][50][51], and herb-derived flavonoids [52] depolarize the pacemaker potential of ICCs in a dose-dependent manner. Depending on the herbal products, different receptors and their signaling pathways are involved in this depolarization process.…”

Section: Effects Of Herbal Products On Icc Receptors and Icc Integritymentioning

confidence: 99%

“…Molecular mechanism Depolarizers of the pacemaker potential of ICCs Gamisoyo-san [14], Citrus unshiu peel extracts [41], Dangkwisoo-san [43] Via M 3 receptor Magnolia officinalis [42] Via M 2 and M 3 receptors Banhasasim-tang [44] Via M 3 and 5-HT 3 receptors Daegeonjung-tang [45] Via M 3 and 5-HT 4 receptors Hesperidin, a citrus flavonoid [52] Via 5-HT 4 receptor Hwangryunhaedok-tang [46], Samhwangsasim-tang [47], Socheongryong-tang [48] Via 5-HT 3 and 5-HT 4 receptor Yukgunja-tang [49] Via ghrelin receptor Leejung-tang [90], Sengmek-san [50] Via PLC pathway Pyungwi-san [51] Via PKC pathway Inhibitors of the pacemaker potential of ICCs Galgeun-tang [26] Via α2-and β1-adrenoceptors Quercetin [91] Via opioid receptor signaling pathways M receptors: muscarinic acetylcholine receptors; 5-HT receptors: 5-hydroxytryptamine receptors; PLC: phospholipase C; PKC: protein kinase C. [53]. is decoction also promotes the numbers of stomach ICCs and the levels of SCF in diabetic rats [54].…”

Section: Medicinal Plants or Decoctions (Reference)mentioning

confidence: 99%

Interstitial Cells of Cajal: Potential Targets for Functional Dyspepsia Treatment Using Medicinal Natural Products

Joung

Choi²,

Son

2021

Evidence-Based Complementary and Alternative Medicine

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Introduction. The pathophysiology of functional dyspepsia (FD) remains uncertain, but the interstitial cells of Cajal (ICCs), pacemakers that regulate gastrointestinal motility, are garnering attention as key modulators and therapeutic targets in FD. This review comprehensively discusses the involvement of ICCs in the pharmacologic actions of FD and as therapeutic targets for herbal products for FD. Methods. A search of the literature was performed using PubMed by pairing “interstitial cells of Cajal” with “medicinal plant, herbal medicine, phytotherapy, flavonoids, or traditional Chinese medicine (TCM).” Results. From the 55 articles screened in the initial survey, 34 articles met our study criteria. The search results showed that herbal products can directly depolarize ICCs to generate pacemaker potentials and increase the expression of c-kit and stem cell factors, helping to repair ICCs. Under certain pathological conditions, medicinal plants also protect ICCs from oxidative stress and/or inflammation-induced impairment. Two representative herbal decoctions (Banhasasim-tang, 半夏泻心汤, and Yukgunja-tang, 六君子汤) have been shown to modulate ICC functions by both clinical and preclinical data. Conclusion. This review strongly indicates the potential of herbal products to target ICCs and suggests that further ICC-based studies would be promising for the development of FD treatment agents.

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“…따라서 본 연구에서는 한방임상에서 타박손상에 주로 사용되는 당귀수산 (Dangkwisoo-san) 20) 과 비만에 관여하 는 생강 21) , 당지질 대사장애에 사용되는 커큐민 이다 (Fig. 3A-C).…”

Section: 포사멸에도 관여함이 잘 알려져 많은 연구실에서 Trpm7unclassified

Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels

Kim

2018

JKOMOR

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Objectives: Metabolic syndrome is correlated with increased cardiovascular risk and characterized by several factors, including visceral obesity, hypertension, insulin resistance, and dyslipidemia. Several members of a large family of nonselective cation entry channels, e.g., transient receptor potential (TRP) melastatin 7 (TRPM7) channels have been associated with the development of cardiovascular diseases. The purpose of this study was to investigate the effects of Dangkwisoo-san, ginger and curcumin on TRPM7 channel. Methods: Human embryonic kidney (HEK) 293 cells stably transfected with the TRPM7 expression vectors were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 5 µg/mL blasticidin, and 0.4 mg/mL zeocin in a humidified 20% O2/10% CO2 atmosphere at 37℃. Whole-cell patch clamp recordings were obtained using an Axopatch 700B amplifier and pClamp v.10.4 software, and signals were digitalized at 5 kHz using Digidata 1422A. Results: Dangkwisoo-san extract (100, 200, 300, 400, and 500 µg/mL) inhibited the outward and inward TRPM7 whole-cell currents at dose dependent manner and the half maximal inhibitory concentration (IC)50 of Dangkwisoo-san was 218.3 µg/mL. Also, ginger extract (100, 200, 300, 400, and 500 µg/mL) inhibited the outward and inward of TRPM7 whole-cell currents in a dose dependent manner and the IC50 of ginger was 877.2 µg/mL. However, curcumin had no effects on TRPM7 whole-cell currents. Conclusions: These results suggest that both Dangkwisoo-san and ginger have good roles to inhibit the TRPM7 channel, suggesting that Dangkwisoo-san and ginger are considered one of the candidate agents for the treatment of metabolic syndrome such as cardiovascular disease.

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Effects of Dangkwisoo-san, a traditional herbal medicine for treating pain and blood stagnation, on the pacemaker activities of cultured interstitial cells of Cajal

Cited by 5 publications

References 18 publications

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine

Interstitial Cells of Cajal: Potential Targets for Functional Dyspepsia Treatment Using Medicinal Natural Products

Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels

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Effects of Dangkwisoo-san, a traditional herbal medicine for treating pain and blood stagnation, on the pacemaker activities of cultured interstitial cells of Cajal

Cited by 5 publications

References 18 publications

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum &lt;i&gt;In Vitro&lt;/i&gt; by Thiamin and Quinine

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum &lt;i&gt;In Vitro&lt;/i&gt; by Thiamin and Quinine

Interstitial Cells of Cajal: Potential Targets for Functional Dyspepsia Treatment Using Medicinal Natural Products

Effects of Dangkwisoo-San, Ginger and Curcumin on Transient Receptor Potential Melastatin 7 Channels

Contact Info

Product

Resources

About

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine

Differential Inhibition of the Rhythm and Amplitude of Acetylcholine-Dependent Contraction in the Murine Jejunum and Ileum <i>In Vitro</i> by Thiamin and Quinine