Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells

Zhang, Yuyan; Zhou, Huifen; Yang, Jiehong; Chen, Xiaoqiang; Nishinari, Katsuyoshi; Wan, Haofang

doi:10.1007/s11655-016-2498-x

Cited by 10 publications

(7 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the pleiotropic effects of DHI on both cardio- and cerebral vasculatures are in accordance with the holistic concept of TCM, they also posed a great challenge in identifying individual chemical compounds responsible for the diverse pharmacological mechanisms. Previous studies by us and others have reported certain major active ingredients of DHI, such as phenolic acids, diterpenes and flavonoids, including salvianolic acid B, danshensu, caffeic acid, rosmarinic acid, kaempferol, protocatechuic acid and hydroxysafflor yellow A 51 , 53 , 54 , 58 , 62 – 68 .…”

Section: Introductionmentioning

confidence: 87%

“…(2) Basic researches indicate that DHI can ameliorate cerebral ischemia-reperfusion injury 40 – 43 , cerebral ischemia damage 44 , myocardial reperfusion injury 45 , 46 , myocardial hypertrophy 47 , cardiac dysfunction 48 and cardiac ventricular remodeling 48 . (3) Pharmacological actions of DHI include antioxidation 49 , 50 , anticoagulation 51 , anti-inflammatory 52 – 54 , anti-fibrosis 55 , anti-angiogenesis 55 , anti-atherosclerosis 56 and anti-diabetes 57 effects. It is also capable of promoting vasodilation 58 , inhibiting vasoconstriction 59 and hyperlipidemia 60 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Network pharmacology exploration reveals endothelial inflammation as a common mechanism for stroke and coronary artery disease treatment of Danhong injection

Lyu

Yan

Liu

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Although Danhong injection (DHI) is the most widely prescribed Chinese medicine for both stroke and coronary artery disease (CAD), its underlying common molecular mechanisms remain unclear. An integrated network pharmacology and experimental verification approach was used to decipher common pharmacological mechanisms of DHI on stroke and CAD treatment. A compound-target-disease & function-pathway network was constructed and analyzed, indicating that 37 ingredients derived from DH (Salvia miltiorrhiza Bge., Flos Carthami tinctorii and DHI) modulated 68 common targets shared by stroke and CAD. In-depth network analysis results of the top diseases, functions, pathways and upstream regulators implied that a common underlying mechanism linking DHI’s role in stroke and CAD treatment was inflammatory response in the process of atherosclerosis. Experimentally, DHI exerted comprehensive anti-inflammatory effects on LPS, ox-LDL or cholesterol crystal-induced NF-κB, c-jun and p38 activation, as well as IL-1β, TNF-α, and IL-10 secretion in vascular endothelial cells. Ten of 14 predicted ingredients were verified to have significant anti-inflammatory activities on LPS-induced endothelial inflammation. DHI exerts pharmacological efficacies on both stroke and CAD through multi-ingredient, multi-target, multi-function and multi-pathway mode. Anti-endothelial inflammation therapy serves as a common underlying mechanism. This study provides a new understanding of DHI in clinical application on cardiovascular and cerebrovascular diseases.

show abstract

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Network pharmacology exploration reveals endothelial inflammation as a common mechanism for stroke and coronary artery disease treatment of Danhong injection

Lyu

Yan

Liu

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…This study investigated the pharmacokinetic analysis of single and combined medication of PAL and HSYA, which are the 2 main parts of DHI. The previous studies have shown the pharmacokinetics of PAL and HSYA by using them either alone or combined with other drugs in normal rats [24][25][26]. The corresponding mechanism of the single and combined application in hyperlipidemia model, however, has not been reported.…”

Section: Discussionmentioning

confidence: 96%

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Zhou¹,

Li²,

Jin³

et al. 2018

Pharmacology

View full text Add to dashboard Cite

Background: Protocatechuic aldehyde (PAL) and hydroxysafflor yellow A (HSYA) are 2 effective ingredients of Danhong Injection, which is extensively used for the clinical treatment of cardio-cerebrovascular diseases. This study aims to investigate the pharmacokinetic differences between single and combined medication of PAL and HSYA and analyze the interaction of the above effective components in hyperlipidemia rats. Methods: Thirty male SD rats were randomly divided into the control group (n = 6) and the model group (n = 24). The hyperlipidemia model was established by feeding with superfatted forage. The successful model rats were then randomly divided into the PAL group (16 mg/kg), the HSYA group (10 mg/kg), and the combination group (16 mg/kg + 10 mg/kg). Administration through tail-vein, and orbital blood was sampled at different time points. The mass concentration of PAL and HSYA was determined by high performance liquid chromatography (HPLC-DAD). Analysis of pharmacokinetic parameters was conducted by using DAS 3.2.6 software and SPSS 19.0 statistical analysis software. Results: According to the parameters of statistical moment of non-compartmental model, there was a significant difference in plasma clearance (CL) between the PAL group and the drug combination group (p < 0.01), as well as in the area under the first moment of the plasma concentration-time curve and the elimination half-life (t_1/2) between the HSYA group and the drug combination group (p < 0.01) but no obvious differences about the blood concentration time curve area, the average dwell time (MRT), and the peak concentration (C_max; p > 0.05). Conclusion: The combined medication of PAL and HSYA could increase the plasma CL significantly and have a great influence on the absorption of HSYA in rats with hyperlipidemia.

show abstract

“…In addition, DHI reduces hypoxia-induced damage in venous endothelial cells. Compared with the hypoxic group, DHI increases levels of 6-keto-PGF1α and NO, as well as the activity of SOD and t-PA , and decreases levels of endothelin-1 (ET-1) and MDA, PAI activity, as well as the apoptosis rate [117].…”

Section: Experimental Studies and Molecular Mechanismsmentioning

confidence: 89%

Danhong injection in cardiovascular and cerebrovascular diseases: Pharmacological actions, molecular mechanisms, and therapeutic potential

Feng

Wang

et al. 2019

Pharmacological Research

103

View full text Add to dashboard Cite

Cardiovascular and cerebrovascular diseases are the main cause of mortality worldwide, currently with less than optimum therapeutic options. Danhong injection (DHI) is a medicinal preparation based on two eminent Chinese herbal medicines, Salviae Miltiorrhizae (Dan Shen; family: Lamiaceae) and Flos Carthami (Hong Hua; family: Compositae/Asteraceae). DHI has been mainly used in the clinical therapy of cardiovascular (such as acute coronary syndrome and angina pectoris) and cerebrovascular diseases (such as stroke) in China for many years. The pharmacological properties of DHI include anti-inflammatory, anti-oxidant, anti-coagulatory, hypolipidemic, anti-apoptotic, vasodilatory, and angiogenesis-promoting actions. DHI offers a safe and effective therapeutic agent against cardiovascular and cerebrovascular diseases by modulating multiple disease-relevant signaling pathways and molecular targets. Herein, we provide a comprehensive review of the phytochemistry, therapeutic effects, molecular mechanisms, and adverse reactions of DHI in cardiovascular and cerebrovascular diseases. We also highlight the latest pharmacological advances and therapeutic potential of this promising herb-derived cardiovascular drug preparation.

show abstract

Effects of Danhong Injection (丹红注射液) and its main components on anticoagulation and fibrinolysis in cultured vein endothelial cells

Abstract: Danhong Injection and its main components played an important role in protecting primary VECs from hypoxic damage by regulating the secretion and vasomotor function of VECs. The function of Danhong Injection was most remarkable.

Cited by 10 publications

References 28 publications

Network pharmacology exploration reveals endothelial inflammation as a common mechanism for stroke and coronary artery disease treatment of Danhong injection

Network pharmacology exploration reveals endothelial inflammation as a common mechanism for stroke and coronary artery disease treatment of Danhong injection

Pharmacokinetic Study on Protocatechuic Aldehyde and Hydroxysafflor Yellow A of Danhong Injection in Rats with Hyperlipidemia

Danhong injection in cardiovascular and cerebrovascular diseases: Pharmacological actions, molecular mechanisms, and therapeutic potential

Contact Info

Product

Resources

About