Effects of decellularized extracellular matrix derived from Jagged1-treated human dental pulp stem cells on biological responses of stem cells isolated from apical papilla

Phothichailert, Suphalak; Nowwarote, Nunthawan; Fournier, Benjamin; Trachoo, Vorapat; Roytrakul, Sittiruk; Namangkalakul, Worachat; Osathanon, Thanaphum

doi:10.3389/fcell.2022.948812

Cited by 6 publications

(2 citation statements)

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“…Decellularized ECM basically preserves the microenvironment and fibrin, mimics natural tissues chemically and mechanically, provides mechanical support for cell seeding and biological 3D carriers, and is considered a suitable natural scaffold material for endodontic tissue engineering [32] . Dental pulpderived decellularized ECM, which produces scaffolds that support the proliferation, differentiation, and regulation of mineralization [33,34] of stem cells, has no apparent cytotoxicity. Animal experiments demonstrated that decellularized ECM containing bone morphogenetic protein 4 (BMP-4) bound to DPSCs, and vascularized pulp histogenesis was observed in nude mice [35] .…”

Section: Acellular Ecmmentioning

confidence: 99%

Progress in application of natural scaffold materials in pulp regeneration

2023

FMSR

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Endodontic and periapical diseases are two of the most common oral diseases and usually cause irreversible damage to the pulp due to severe irritation, such as dental caries, accidental trauma, or iatrogenic causes. The typical dental pulp treatment method is root canal therapy, but the loss of dental pulp tissue changes the function of dental pulp, and may cause a series of complications. Pulp regeneration therapy uses the principle of tissue engineering to replace the diseased pulp tissue with new pulp tissue so as to achieve the purpose of restoring the normal physiological function of the pulp. Scaffolds are one of the important elements of tissue engineering, and a variety of scaffolds have been found to potentially be used in pulp regeneration. Naturally derived scaffold materials have good biocompatibility and rapid degradation rate and are widely used in endodontic tissue engineering.

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Section: Acellular Ecmmentioning

confidence: 99%

Progress in application of natural scaffold materials in pulp regeneration

2023

FMSR

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“…Recent studies have illustrated the regenerative potential of decellularized extracellular matrix (dECM) obtained from DPSCs for bone tissue regeneration, as it promotes mineralization (8). In addition, dECM generated from immobilized Jagged1-treated hDPSCs increases the odonto/osteogenic differentiation of stem cells isolated from the apical papilla (SCAPs) (9).…”

Section: Introductionmentioning

confidence: 99%

Decellularized Extracellular Matrix Derived from Dental Pulp Stem Cells Promotes Gingival Fibroblast Adhesion and Migration

Nowwarote,

Chahlaoui,

Petit

et al. 2024

Preprint

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Background: Decellularized extracellular matrix (dECM) has been proposed as a useful source of biomimetic materials for regenerative medicine due to its biological properties that regulate cell behaviors. The present study aimed to investigate the influence of decellularized ECM derived from dental pulp stem cells (DPSCs) on gingival fibroblast (GF) cell behaviors. Cells were isolated from dental pulp and gingival tissues. ECM was derived from culturing dental pulp stem cells in growth medium supplemented with ascorbic acid. A bioinformatic database of the extracellular matrix was constructed using Metascape. GFs were reseeded onto dECM, and their adhesion, spreading, and organization were subsequently observed. The migration ability of the cells was determined using a scratch assay. Protein expression was evaluated using immunofluorescence staining. Results: Type 1 collagen and fibronectin were detected on the ECM and dECM derived from DPSCs. Negative phalloidin and nuclei were noted in the dECM. The proteomic database revealed enrichment of several proteins involved in ECM organization, ECM–receptor interaction, and focal adhesion. Compared with those on the controls, the GFs on the dECM exhibited more organized stress fibers. Furthermore, cultured GFs on dECM exhibited significantly enhanced migration and proliferation abilities. Interestingly, GFs seeded on dECM showed upregulation of FN1, ITGB3, and CTNNB1 mRNA levels. Conclusions: ECM derived from DSPCs generates a crucial microenvironment for regulating GF adhesion, migration and proliferation. Therefore, decellularized ECM from DPSCs could serve as a matrix for oral tissue repair.

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