Effects of defatted yellow mealworm (Tenebrio molitor) on growth performance, intestine, and liver health of turbot (Scophthalmus maximus)

Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways

“…According to our previous study, the basal diet (FM diet) containing 62% fishmeal as the sole protein source was formulated ( Bai et al., 2023 ). Five isonitrogenous (48%) and isolipidic (12%) experimental diets were formulated using CAP to replace fishmeal at levels of 15% (CAP15), 30% (CAP30), 45% (CAP45), 60% (CAP60), and 75% (CAP75).…”

“… Item Forward (5′ to 3′) Reverse (5′ to 3′) GenBank no. myod AGGTCAPACGACGCCTTCGAGAC GCAPGGGACTCGATGTAGCTTATGG MH174948.1 myf 5 GTGGCAPACAPTCTGAACGCAPAACTAC GGAGAGACAPCTGGAGGCTGGAG MN072640 myog CGGCGTCTGAAGAAGGTGAATGAG TGGAGCCTCTCGATATACTGGATGG MH174947.1 mrf 4 GACCTCTTCGAGACCAPACGCTTATC CGTTGTACAPGCGGCAPTGTCCAPG MN072641 pax 7 CCCAPGACAPTCTACAPCCAPGAGAGGAG GCCTGTTGCTGAACCAPCAPCCTG MN072642 murf 1 CAPCGAAGACGAACGCAPTCAPACAPTC TCTGGCTCTGGTAGACGGCAPAG MH174966 maf bx ACGCTCTACCTCTCGCTCTGTG CGCAPGCCAPGGTGTTGATGTTCC MH174969 ctsd CTGCTTGCTTCAPCCAPCAPAATATAA GACAPAACTGCCAPGATCCAPTACT KY593341.1 ctsl GACAPGCTGAGGAGATCAPATGAG GAGCCAPCTCGCTTCCTTAAA EU077233.1 rpsd 1 AACAPCAPGGAAGCAPGCAPGAAC ACGGCAPGTGATGGTCTCTC myod = myogenic differentiation; myf 5 = myogenic factor 5; myog = myogenin; mrf 4 = myogenic regulatory factor 4; pax 7 = paired box 7; murf 1 = muscle ring finger protein 1; maf bx = muscle atrophy F box; ctsd = cathepsin D; ctsl = cathepsin L; rpsd = RNA polymerase II subunit D. 1 The rpsd primer sequences referred to Bai et al. (2023) .…”