Effects of Degeneration on the Elastic Modulus Distribution in the Lumbar Intervertebral Disc

Umehara, Shinji; Tadano, Shigeru; Abumi, Kuniyoshi; Katagiri, K.; Kaneda, Kiyoshi; Ukai, Takayoshi

doi:10.1097/00007632-199604010-00007

Cited by 87 publications

(65 citation statements)

References 27 publications

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“…15,16 The viscoelastic properties of the goat NP are largely in line with earlier investigations of human, 17,18 pig, 19 and sheep 20 NP, as summarized in Table 1. These findings underscore the potential of the goat as an animal model for disc herniation studies.…”

Section: Discussionsupporting

confidence: 82%

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Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Bron

Koenderink

Everts

et al. 2008

Journal Orthopaedic Research

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Lumbar discectomy is an effective therapy for neurological decompression in patients suffering from sciatica due to a herniated nucleus pulposus (NP). However, high numbers of patients suffering from persisting postoperative low back pain have resulted in many strategies targeting the regeneration of the NP. For successful regeneration, the stiffness of scaffolds is increasingly recognized as a potent mechanical cue for the differentiation and biosynthetic response of (stem) cells. The aim of the current study is to characterize the viscoelastic properties of the NP and to develop dense collagen scaffolds with similar properties. The scaffolds consisted of highly dense (0.5%-12%) type I collagen matrices, prepared by plastic compression. The complex modulus of the NP was 22 kPa (at 10 rad s À1 ), which should agree with a scaffold with a collagen concentration of 23%. The loss tangent, indicative of energy dissipation, is higher for the NP (0.28) than for the scaffolds (0.12) and was not dependent of the collagen density. Gamma sterilization of the scaffolds increased the shear moduli but also resulted in more brittle behavior and a reduced swelling capacity. In conclusion, by tuning the collagen density, we can approach the stiffness of the NP. Therefore, dense collagen is a promising candidate for tissue engineering of the NP that deserves further study, such as the addition of other proteins. ß

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Section: Discussionsupporting

confidence: 82%

“…Earlier studies have shown that G 00 increases in case of disc degeneration. 18 In this study, we used a plastic compression technique to develop collagen I-based scaffolds with varying concentrations and viscoelastic properties. This is a novel technique that was first described by Brown et al 21 as a form of cell-independent engineering.…”

Section: Discussionmentioning

confidence: 99%

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Bron

Koenderink

Everts

et al. 2008

Journal Orthopaedic Research

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“…Furthermore, the gels were highly elastic, with storage moduli generally 4-5 times larger than loss moduli. To put these data into perspective, typical compressive moduli for native tissue are: nucleus pulposus, 3-310 kPa [33][34][35] ; annulus fibrosus, 29-740 kPa [34][35][36] ; articular cartilage, 0.31-1.237 MPa [37][38][39] ; and meniscus, 0.605-1.03 MPa. 40,41 From these compressive modulus values of native tissues, the 5-6% (w/v) low acyl gellan gum with complex compressive moduli of 324.1 6 6.3 and 407.9 6 10.0 kPa, respectively could be a good candidate for nucleus pulposus and annulus fibrosus replacement, and nearly sufficient for meniscus.…”

Section: Discussionmentioning

confidence: 99%

Optimizing gelling parameters of gellan gum for fibrocartilage tissue engineering

et al. 2011

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Gellan gum is an attractive biomaterial for fibrocartilage tissue engineering applications because it is cell compatible, can be injected into a defect, and gels at body temperature. However, the gelling parameters of gellan gum have not yet been fully optimized. The aim of this study was to investigate the mechanics, degradation, gelling temperature, and viscosity of low acyl and low/high acyl gellan gum blends. Dynamic mechanical analysis showed that increased concentrations of low acyl gellan gum resulted in increased stiffness and the addition of high acyl gellan gum resulted in greatly decreased stiffness. Degradation studies showed that low acyl gellan gum was more stable than low/high acyl gellan gum blends. Gelling temperature studies showed that increased concentrations of low acyl gellan gum and CaCl₂ increased gelling temperature and low acyl gellan gum concentrations below 2% (w/v) would be most suitable for cell encapsulation. Gellan gum blends were generally found to have a higher gelling temperature than low acyl gellan gum. Viscosity studies showed that increased concentrations of low acyl gellan gum increased viscosity. Our results suggest that 2% (w/v) low acyl gellan gum would have the most appropriate mechanics, degradation, and gelling temperature for use in fibrocartilage tissue engineering applications.

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“…Despite this complication, there is consistent evidence that annulus UTS and modulus (normalised stiffness) increase radially outwards, from the inner AF to the disc periphery [3][4][5][6]. This can be attributed to the increasing volume fraction of large and strong collagen Type I fibres in the periphery [7].…”

Section: Introductionmentioning

confidence: 92%

Correlation between biomechanical properties of the annulus fibrosus and magnetic resonance imaging (MRI) findings

Shan

Liu

et al. 2015

Eur Spine J

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Effects of Degeneration on the Elastic Modulus Distribution in the Lumbar Intervertebral Disc

Abstract: The distribution of elastic moduli is much different between discs with and without degeneration.

Cited by 87 publications

References 27 publications

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Rheological characterization of the nucleus pulposus and dense collagen scaffolds intended for functional replacement

Optimizing gelling parameters of gellan gum for fibrocartilage tissue engineering

Correlation between biomechanical properties of the annulus fibrosus and magnetic resonance imaging (MRI) findings

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