Effects of Delay in the Snap Freezing of Colorectal Cancer Tissues on the Quality of DNA and RNA

Hong, Sang Hyun; Baek, Hong-Sun; Jang, Kyu Yun; Chung, Myoung Ja; Moon, Woo Sung; Kang, Myoung Jae; Lee, Dong Geun; Park, Ho Sung

doi:10.3393/jksc.2010.26.5.316

Cited by 46 publications

(30 citation statements)

References 12 publications

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“…Overall, 93% of studies reported at least two diagnostic and two predictive measures of quality,12 17–24 26–30 with the majority of studies (53%) reporting at least six of the nine diagnostic and/or predictive quality measures 12 18 20–22 27–29. Moreover, of those, 25% of studies reported at least seven of the nine measures of interest 21 28…”

Section: Resultsmentioning

confidence: 99%

Quality assessment and preservation of RNA from biobank tissue specimens: a systematic review

2015

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It is well recognised that genomic, proteomic and biomarker studies require properly annotated and well-characterised biospecimens. Consequently, this necessitates biobanks to collect, store and distribute biospecimens under stringent quality control and assurance measures. However, despite this realisation, there remains a lack of standardisation in quality management among biobanks and consensus as to which quality indicators provide the optimal molecular diagnostic performance tools and information for biospecimens. In an attempt to identify key factors that predict tissue specimen integrity and quality, this systematic review investigated the measures reported in the literature, which characterised the collection, processing and storage of high-quality tissue specimens. Our findings demonstrated RNA integrity, alone, may not be an effective measure of tissue quality. Furthermore, the frequently reported parameters related to biospecimen integrity, such as storage time, temperature, time to cryopreservation and tissue morphology were also not effective indicators of quality control and assurance. These findings suggest that it is unlikely that a single marker will provide the optimal diagnostic and performance information for biospecimens, but rather, a panel of markers assessing the molecular integrity of the lifespan of the biospecimen is required. Further work is needed to identify which factors predict specimen integrity and quality in biobanked tissue specimens.

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Section: Resultsmentioning

confidence: 99%

Quality assessment and preservation of RNA from biobank tissue specimens: a systematic review

2015

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“…This may explain the relatively low number of samples containing more than 60% tumor nuclei we obtained compared with previous studies. 18,19 Taking samples from a more central location in the tumor would likely have increased this value, but might also increase the number of samples with large necrosis. Although our FFPE and fresh frozen tissue came from the same biopsy, some differences between the two pieces could be present.…”

Section: Discussionmentioning

confidence: 97%

“…A more accurate image of the content in the fresh frozen pieces would likely be obtained if mirror-image slides were prepared from each end of the fresh frozen piece. 18,20 However, tissue morphology can be distorted during the liquid nitrogen snap freezing process.…”

Section: Discussionmentioning

confidence: 99%

Impact of in Vivo Ischemic Time on RNA Quality—Experiences from a Colon Cancer Biobank

Olsen

Eiholm

et al. 2015

Biopreservation and Biobanking

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Considerable effort has been made to improve differentiated diagnostics as well as personalized treatment for colorectal cancer patients. High-quality fresh frozen tissue is often required to investigate relevant molecular signatures in these patients. In RNA expression studies, the "RNA integrity number" is widely accepted as a reliable marker of RNA quality. Here, we investigate the feasibility of obtaining high-quality tissue from a colon cancer biobank and the impact of in vivo ischemic time and various technical and clinicopathological factors on RNA quality. Biopsies were obtained immediately following the tumor removal. The time from clamping the main arterial supply to resection and removal of the tumor was used to estimate the in vivo ischemic time. We did not observe a significant difference in RNA quality between normal tissue and tumor tissue. We observed a significant correlation between in vivo ischemic time and RNA quality in normal tissue (r = -0.24, p<0.001) but not in tumor tissue. Male gender and laparoscopic procedure were also significantly associated with lower RNA quality in normal tissue only. In tumor tissue, poor differentiation was associated with low RNA quality. In conclusion, in vivo ischemic time, surgical procedure, and gender have minor but significant effects on the quality of RNA from normal colon tissue but not tumor tissue. Poorly differentiated tumors are associated with lower RNA quality. Although its impact is low, it can still be considered to note in vivo ischemic time in colon cancer specimen procurement.

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“…Some studies report that any delay in getting the sample from the living state to a preserved state (frozen, in formalin (FFPE) or RNAlater) will decrease the quality of the sample (Hong et al, 2010). There are other studies that indicate that there is at least a 16 hour window in which the sample collection and the QC metrics of BioAnalyzer assessment do not show any degradation (Micke et al, 2006).…”

Section: Sample Collectionmentioning

confidence: 99%

Novel Tissue Types for the Development of Genomic Biomarkers

Sergueeva¹,

Collins²,

Dow³

et al. 2012

Biomarker

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Effects of Delay in the Snap Freezing of Colorectal Cancer Tissues on the Quality of DNA and RNA

Cited by 46 publications

References 12 publications

Quality assessment and preservation of RNA from biobank tissue specimens: a systematic review

Quality assessment and preservation of RNA from biobank tissue specimens: a systematic review

Impact of in Vivo Ischemic Time on RNA Quality—Experiences from a Colon Cancer Biobank

Novel Tissue Types for the Development of Genomic Biomarkers

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