Effects of deletion of the receptor CrlA on Dictyostelium aggregation and MPBD-mediated responses are strain dependent and not evident in strain Ax2

Narita, Takaaki B.; Schaap, Pauline; Saito, Tamao

doi:10.1093/femsle/fnx022

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…CrlA was reported to detect the polyketide MPBD (4-methyl-5-pentylbenzene-1,3-diol), which stimulates both aggregation competence and release of the peptide SDF-1 (spore differentiation factor-1), which then promotes spore and stalk differentiation ( Anjard et al , 2011 ). However, other workers found that CrlA does not mediate MPBD induction of aggregation competence and is not needed for normal spore differentiation in AX2 wild type cells ( Narita et al , 2017 ). Clade 3 contains the four D.dis cAMP receptors (CarA, CarB, CarC and CarD).…”

Section: Resultsmentioning

confidence: 99%

Phylogeny-wide analysis of G-protein coupled receptors in social amoebas and implications for the evolution of multicellularity

Hall¹,

Kelly²,

Schaap³

et al. 2023

Open Res Europe

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G-protein coupled receptors (GPCRs) are seven-transmembrane proteins and constitute the largest group of receptors within eukaryotes. The presence of a large set of GPCRs in the unicellular Amoebozoa was surprising and is indicative of the largely undiscovered environmental sensing capabilities in this group. Evolutionary transitions from unicellular to multicellular lifestyles, like we see in social amoebas, have occurred several times independently in the Amoebozoa, and GPCRs may have been co-opted for new functions in cell-cell communication. Methods We have analysed a set of GPCRs from fully sequenced Amoebozoan genomes by Bayesian inference, compared their phylogenetic distribution and domain composition, and analysed their temporal and spatial expression patterns in five species of dictyostelids. Results We found evidence that most GPCRs are conserved deeply in the Amoebozoa and are probably performing roles in general cell functions and complex environmental sensing. All families of GPCRs (apart from the family 4 fungal pheromone receptors) are present in dictyostelids with family 5 being the largest and family 2 the one with the fewest members. For the first time, we identify the presence of family 1 rhodopsin-like GPCRs in dictyostelids. Some GPCRs have been amplified in the dictyostelids and in specific lineages thereof and through changes in expression patterns may have been repurposed for signalling in multicellular development. Discussion Our phylogenetic analysis suggests that GPCR families 1, 2 and 6 already diverged early in the Amoebozoa, whereas families 3 and 5 expanded later within the dictyostelids. The family 6 cAMP receptors that have experimentally supported roles in multicellular development in dictyostelids (carA-carD; tasA/B) originated at the root of all dictyostelids and only have weakly associated homologs in Physarum polycephalum. Our analysis identified candidate GPCRs which have evolved in the dictyostelids and could have been co-opted for multicellular development.

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Section: Resultsmentioning

confidence: 99%

Phylogeny-wide analysis of G-protein coupled receptors in social amoebas and implications for the evolution of multicellularity

Hall¹,

Kelly²,

Schaap³

et al. 2023

Open Res Europe

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“…CrlA-mutant with the Ax2 background showed normal cell aggregation, unlike the stlAmutant. This indicates that MPBD activity in the early development of Ax2 does not require CrlA (Narita et al, 2017).…”

Section: Mpbd: Biosynthesis and Functionmentioning

confidence: 87%

Small molecules and cell differentiation in Dictyostelium discoideum

Araki¹,

Saito²

2019

Int. J. Dev. Biol.

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Dictyostelium is a microorganism found in soils that are known as the battle fields of chemical warfare. Genome analysis of Dictyostelium revealed that it has great potential for the production of small molecules, including secondary metabolites such as polyketides and terpenes. Polyketides are a large family of secondary metabolites which have a variety of structures. In accordance with their structural variety, polyketides have a plethora of biological activities, including antimicrobial, antifungal, and antitumor activities. Unsurprisingly, they have exceptional medical importance. Polyketides in nature work as protective compounds and /or function in pheromonal communication. Terpenes belong to another family of structurally diverse secondary metabolites which play roles in ecological interactions, including defence against predators and formation of mutually beneficial alliance with other organisms. Polyketides and terpenes work as intra-or inter-species signalling compounds, i.e. they play the role of a chemical language. However, in Dictyostelium, they work as paracrine signalling compounds which control the organism's multicellular morphogenesis. This review is primarily focused on the small molecules that regulate pattern formation in the slug stage of the organism and their biosynthetic pathways. Current in vivo understandings of polyketide DIF-1 induced cell differentiation and DIF-1-dependent/independent pathways are also discussed.

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The polyketide synthase StlA is involved in inducing aggregation in Polysphondylium violaceum

Yamasaki

Araki

Narita

2022

Bioscience, Biotechnology, and Biochemistry

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In the social amoeba Dictyostelium discoideum, the polyketide MPBD (4-methyl-5-pentylbenzene-1,3-diol) regulates the gene expressions of cAMP signaling to make cells aggregation-competent and also induces spore maturation. The polyketide synthase StlA is responsible for MPBD biosynthesis in D. discoideum and appears to be conserved throughout the major groups of the social amoeba (Dictyostelia). In this study, we analyzed the function of StlA in Polysphondylium violaceum by identifying the gene sequence and creating the knockout mutants. We found that Pv-stlA– mutants had defects only in cell aggregation but not in spore maturation, indicating that the function of StlA in inducing spore maturation is species-specific. We also found that MPBD could rescue the aggregation defect in Pv-stlA– mutants whereas the mutants normally exhibited chemotaxis to their chemoattractant, glorin. Our data suggest that StlA is involved in inducing aggregation in P. violaceum by acting on signaling pathways other than chemotaxis in P. violaceum.

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Effects of deletion of the receptor CrlA on Dictyostelium aggregation and MPBD-mediated responses are strain dependent and not evident in strain Ax2

Cited by 4 publications

References 31 publications

Phylogeny-wide analysis of G-protein coupled receptors in social amoebas and implications for the evolution of multicellularity

Phylogeny-wide analysis of G-protein coupled receptors in social amoebas and implications for the evolution of multicellularity

Small molecules and cell differentiation in Dictyostelium discoideum

The polyketide synthase StlA is involved in inducing aggregation in Polysphondylium violaceum

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