Effects of Delipidation with Forskolin During In Vitro Culture of Bovine Embryos and Recipient Synchronization on Pregnancy Rates.

Barceló-Fimbres, M.; Anchondo-Garay, Alfredo; López-Franco, Esther; García-Quiñonez, Sara; Antillón-Ruiz, Javier; Rodríguez-Almeida, Felipe Alonso; Seidel, G. E.

doi:10.1093/biolreprod/83.s1.677

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent experiments, Barceló-Fimbres et al (2010) produced IVP embryos with forskolin followed by cryopreservation and did not find satisfactory results in the pregnancy rate. One explanation for this low effect of forskolin on the pregnancy rate may be that the authors used a higher serum concentration than in the present experiment (up 2.5%), and thus, the lipolytic agent was not sufficient to induce lipolysis at the concentration used (10 M).…”

Section: Discussionmentioning

confidence: 99%

Forskolin effect on the cryosurvival ofin vitro-produced bovine embryos in the presence or absence of fetal calf serum

Paschoal

Sudano

Guastali

et al. 2012

Zygote

View full text Add to dashboard Cite

The objective of this study was to assess the viability and cryotolerance of zebu embryos produced in vitro with or without the addition of fetal calf serum (FCS) and forskolin (F). Embryos produced in vivo were used as a control. Presumptive zygotes were cultured in modified synthetic oviductal fluid supplemented with amino acids (SOFaa), bovine serum albumin (BSA) and with (2.5%) or without (0%) FCS. On day 6 of growth, the embryos from each group were divided into treatments with or without 10 μM F to induce embryonic lipolysis, comprising a total of four experimental groups: 2.5% FCS, 0% FCS, 2.5% + F and 0% + F. For vitrification, embryos were exposed to vitrification solution 1 (5 M EG (ethylene glycol)) for 3 min and then transferred to vitrification solution 2 (7 M EG, 0.5 M galactose solution and 18% (w/v) Ficoll 70) before being introduced to liquid nitrogen. The presence of FCS in the culture medium resulted in the production of embryos with a similar rate of damaged cells compared with in vivo-produced embryos. After vitrification, the 2.5% FCS group had a significantly higher rate of damaged cells when compared with the other groups (P < 0.05). The results of this experiment indicated that the omission of FCS and the addition of forskolin do not have deleterious effect on embryo production rates. In addition, embryos produced in the presence of FCS had greater sensitivity to cryopreservation, but this effect was reversed when forskolin was added to the medium, which improved embryo survival without affecting embryo development and quality after vitrification.

show abstract

Section: Discussionmentioning

confidence: 99%

Forskolin effect on the cryosurvival ofin vitro-produced bovine embryos in the presence or absence of fetal calf serum

Paschoal

Sudano

Guastali

et al. 2012

Zygote

View full text Add to dashboard Cite

show abstract

Treatment with chemical delipidation forskolin prior to cryopreservation improves the survival rates of swamp buffalo (Bubalus bubalis) and bovine (Bos indicus) in vitro produced embryos

et al. 2018

View full text Add to dashboard Cite

Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells

Ferreira¹,

Jarmusch²,

Pirro³

et al. 2015

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Lipids play fundamental roles in mammalian embryo preimplantation development and cell fate. Triacylglycerol accumulates in oocytes and blastomeres as lipid droplets, phospholipids influence membrane functional properties, and essential fatty acid metabolism is important for maintaining the stemness of cells cultured in vitro. The growing impact that lipids have in the field of developmental biology makes analytical approaches to analyse structural information of great interest. This paper describes the concept and presents the results of lipid profiling by mass spectrometry (MS) of oocytes and preimplantation embryos, with special focus on ambient ionisation. Based on our previous experience with oocytes and embryos, we aim to convey that ambient MS is also valuable for stem cell differentiation analysis. Ambient ionisation MS allows the detection of a wide range of lipid classes (e.g. free fatty acids, cholesterol esters, phospholipids) in single oocytes, embryos and cell pellets, which are informative of in vitro culture impact, developmental and differentiation stages. Background on MS principles, the importance of underused MS scan modes for structural analysis of lipids, and statistical approaches used for data analysis are covered. We envisage that MS alone or in combination with other techniques will have a profound impact on the understanding of lipid metabolism, particularly in early embryo development and cell differentiation research.

show abstract

Effects of Delipidation with Forskolin During In Vitro Culture of Bovine Embryos and Recipient Synchronization on Pregnancy Rates.

Cited by 3 publications

References 0 publications

Forskolin effect on the cryosurvival ofin vitro-produced bovine embryos in the presence or absence of fetal calf serum

Forskolin effect on the cryosurvival ofin vitro-produced bovine embryos in the presence or absence of fetal calf serum

Treatment with chemical delipidation forskolin prior to cryopreservation improves the survival rates of swamp buffalo (Bubalus bubalis) and bovine (Bos indicus) in vitro produced embryos

Ambient ionisation mass spectrometry for lipid profiling and structural analysis of mammalian oocytes, preimplantation embryos and stem cells

Contact Info

Product

Resources

About