2021
DOI: 10.53388/tmr20210219220
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Effects of Dendrobium candidum polysaccharides on microRNA-125b and mitogen-activated protein kinase signaling pathways in diabetic cataract rats

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Cited by 5 publications
(4 citation statements)
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“…Apart from type 1 and type 2 diabetes, diabetic complications are also recognized as a severe health concern. Dendrobium officinale polysaccharides were demonstrated to ameliorate diabetic cataracts in rats, and it could reduce the severity of the opacity of the lens by downregulating the microRNA-125b and MAPK signaling pathways, in which the level of microRNA-125b was positively correlated with the levels of ERK1, ERK2, Raf, and Ras [71].…”
Section: Oxidative Medicine and Cellular Longevitymentioning
confidence: 99%
“…Apart from type 1 and type 2 diabetes, diabetic complications are also recognized as a severe health concern. Dendrobium officinale polysaccharides were demonstrated to ameliorate diabetic cataracts in rats, and it could reduce the severity of the opacity of the lens by downregulating the microRNA-125b and MAPK signaling pathways, in which the level of microRNA-125b was positively correlated with the levels of ERK1, ERK2, Raf, and Ras [71].…”
Section: Oxidative Medicine and Cellular Longevitymentioning
confidence: 99%
“…Finally, the signals were detected by adding enhanced chemiluminescence (ECL) substrate (Millipore, Billerica, MA, USA) and visualized by a Tanon 5200 Imaging Analysis System (Tanon, Shanghai, China). Relative protein levels were determined by densitometry analysis using the Image-Pro Plus software [ 20 ]. The relative protein expression was normalized to β -actin.…”
Section: Methodsmentioning
confidence: 99%
“…e proteins were separated using SDS-PAGE electrophoresis, transferred to PVDF membranes, blocked with 5% skimmed milk for 2 h at room temperature, and incubated with rabbit antirat primary antibody P65 (1 : 1,000), p-P65 (1 : 1,000), JNK1/ 2 (1 : 2,000), SREBP1 (1 : 1,000), leptin (1 : 1,000), Fas (1 : 1,000), cytochrome C (1 : 2,000), GSK3β (1 : 1,000), CYP2E1 (1 : 1,000), and β-action (1 : 2,000) overnight at 4 °C. After washing the membrane, the membrane was incubated with the secondary antibody (goat anti-rabbit IgG; 1 : 8,000) at room temperature for 2 h. After washing the membrane with Tris-buffered saline, enhanced chemiluminescence was added to develop and detect the bands with Image-Pro plus 6.0 software for quantitative analysis of the grayscale values [14].…”
Section: Western Blottingmentioning
confidence: 99%