Effects of depleted uranium on the reproductive success and F1 generation survival of zebrafish (Danio rerio)

Bourrachot, Stéphanie; Brion, François; Pereira, Sandrine; Floriani, Magali; Camilleri, Virginie; Cavalié, Isabelle; Palluel, Olivier; Adam-Guillermin, Christelle

doi:10.1016/j.aquatox.2014.04.018

Cited by 38 publications

(14 citation statements)

References 46 publications

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“…Here, survival among control larvae was ~60% from harvested eggs to adulthood, which is typical survival for the breeding pairs used here and comparable to rates achieved by our own group and elsewhere (e.g. Bourrachot et al, 2014). There was no loss due to death or malformation after 10-dpf (100% of 10-dpf fish survived to adulthood), which is also consistent with previous studies.…”

Section: Resultssupporting

confidence: 88%

Persistent behavioral effects following early life exposure to retinoic acid or valproic acid in zebrafish

et al. 2016

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BACKGROUND Moderate to severe dysregulation in retinoid signaling during early development is associated with a constellation of physical malformations and/or neural tube defects, including spina bifida. It is thought that more subtle dysregulation of this system, which might be achievable via dietary (i.e. hypervitaminosis A) or pharmacological (i.e. valproic acid) exposure in humans, will manifest on behavioral domains including sociability, without overt physical abnormalities. METHODS During early life, zebrafish were exposed to low doses of two chemicals that disrupt retinoid signaling. From 0-5 dpf, larvae were reared in aqueous solutions containing retinoic acid (0, 0.02, 0.2 or 2 nM) or valproic acid (0, 0.5, 5.0 or 50 uM). One cohort of zebrafish was assessed using a locomotor activity screen at 6-dpf; another was reared to adulthood and assessed using a neurobehavioral test battery (startle habituation, novel tank exploration, shoaling, and predator escape/avoidance). RESULTS There was no significant increase in the incidence of physical malformation among exposed fish compared to controls. Both retinoic acid and valproic acid exposures during development disrupted larval activity with persisting behavioral alterations later in life, primarily manifesting as decreased social affiliation. CONCLUSIONS Social behavior and some aspects of motor function were altered in exposed fish; the importance of examining emotional or psychological consequences of early life exposure to retinoid acting chemicals is discussed.

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Section: Resultssupporting

confidence: 88%

Persistent behavioral effects following early life exposure to retinoic acid or valproic acid in zebrafish

et al. 2016

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“…More than 50% of the total U burden in the internal organs (i.e., excluding the intestine and gill burdens) was measured in the testes after 20 d of both exposure pathways (Figure ). Uranium accumulation in the testes may confirm the epigenetic effects, transcriptomic changes, and histological damage (at higher levels of U exposure) in this organ detected by Bourrachot et al (), Gombeau et al (), and Armant et al ().…”

Section: Discussionsupporting

confidence: 71%

Uranium transfer and accumulation in organs of Danio rerio after waterborne exposure alone or combined with diet‐borne exposure

Simon

Gagnaire

Sommard

et al. 2018

Enviro Toxic and Chemistry

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Uranium (U) toxicity patterns for fish have been mainly determined under laboratory‐controlled waterborne exposure conditions. Because fish can take up metals from water and diet under in situ exposure conditions, a waterborne U exposure experiment (20 μg L−1, 20 d) was conducted in the laboratory to investigate transfer efficiency and target organ distribution in zebrafish Danio rerio compared with combined waterborne exposure (20 μg L−1) and diet‐borne exposure (10.7 μg g−1). 233Uranium was used as a specific U isotope tracer for diet‐borne exposure. Bioaccumulation was examined in the gills, liver, kidneys, intestine, and gonads of D. rerio. Concentrations in the organs after waterborne exposure were approximately 500 ng g−1 fresh weight, except in the intestine (> 10 μg g−1 fresh wt) and the kidneys (200 ng g−1 fresh wt). No significant difference was observed between waterborne and diet‐borne conditions. Trophic U transfer in organs was found but at a low level (< 10 ng g−1 fresh wt). Surprisingly, the intestine appeared to be the main target organ after both tested exposure modalities. The gonads (57% at 20 d) and the liver (41% at 20 d) showed the highest accumulated relative U burdens. Environ Toxicol Chem 2019;38:90–98. © 2018 SETAC

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“…Mitochondrial and DNA repair enzymes were also perturbed in zebrafish skeletal muscles and brain [17,18], similarly to what was observed in the kidney of mice chronically exposed to uranium [19]. DU bioaccumulates in the gonads of females chronically exposed and is transmitted to the eggs [20,21]. This transfer from the exposed adult to the progeny was proposed to account for the genotoxic effects of uranium.…”

Section: Introductionmentioning

confidence: 88%

Zebrafish exposure to environmentally relevant concentration of depleted uranium impairs progeny development at the molecular and histological levels

Armant¹,

Gombeau²,

Houdigui³

et al. 2017

PLoS ONE

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Uranium is an actinide naturally found in the environment. Anthropogenic activities lead to the release of increasing amounts of uranium and depleted uranium (DU) in the environment, posing potential risks to aquatic organisms due to radiological and chemical toxicity of this radionucleide. Although environmental contaminations with high levels of uranium have already been observed, chronic exposures of non-human species to levels close to the environmental quality standards remain scarcely characterized. The present study focused on the identification of the molecular pathways impacted by a chronic exposure of zebrafish to 20 μg/L of DU during 10 days. The transcriptomic effects were evaluated by the use of the mRNAseq analysis in three organs of adult zebrafish, the brain the testis and the ovaries, and two developmental stages of the adult fish progeny, two-cells embryo and four-days larvae. The results highlight generic effects on the cell adhesion process, but also specific transcriptomic responses depending on the organ or the developmental stage investigated. The analysis of the transgenerational effects of DU-exposure on the four-day zebrafish larvae demonstrate an induction of genes involved in oxidative response (cat, mpx, sod1 and sod2), a decrease of expression of the two hatching enzymes (he1a and he1b), the deregulation of the expression of gene coding for the ATPase complex and the induction of cellular stress. Electron microscopy analysis of skeletal muscles on the four-days larvae highlights significant histological impacts on the ultrastructure of both the mitochondria and the myofibres. In addition, the comparison with the transcriptomic data obtained for the acetylcholine esterase mutant reveals the induction of protein-chaperons in the skeletal muscles of the progeny of fish chronically exposed to DU, pointing towards long lasting effects of this chemical in the muscles. The results presented in this study support the hypothesis that a chronic parental exposure to an environmentally relevant concentration of DU could impair the progeny development with significant effects observed both at the molecular level and on the histological ultrastructure of organs. This study provides a comprehensive transcriptomic dataset useful for ecotoxicological studies on other fish species at the molecular level. It also provides a key DU responsive gene, egr1, which may be a candidate biomarker for monitoring aquatic pollution by heavy metals.

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Effects of depleted uranium on the reproductive success and F1 generation survival of zebrafish (Danio rerio)

Cited by 38 publications

References 46 publications

Persistent behavioral effects following early life exposure to retinoic acid or valproic acid in zebrafish

Persistent behavioral effects following early life exposure to retinoic acid or valproic acid in zebrafish

Uranium transfer and accumulation in organs of Danio rerio after waterborne exposure alone or combined with diet‐borne exposure

Zebrafish exposure to environmentally relevant concentration of depleted uranium impairs progeny development at the molecular and histological levels

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