Effects of Destruxin A on Hemocytes Morphology of Bombyx mori

Fan, Jiqiao; Chen, Xiurun; Hu, Qiongbo

doi:10.1016/s2095-3119(13)60482-7

Cited by 29 publications

(24 citation statements)

References 20 publications

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“…The effect of extensively applied and commercial-grade insecticides such as iminoctadine, fentrothion, ethion and destruxin A, on the silkworm, including subacute and delayed toxicity, carbohydrate and protein metabolism in hemolymph and fat body, digestive enzymes, and food utilization were investigated [ 4 , 21 , 22 , 23 , 24 ]. In addition, the effects of some extracts from plants were also assayed.…”

Section: Resultsmentioning

confidence: 99%

Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx mori L.

Chen

et al. 2015

Toxins

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The silkworm Bombyx mori L. (B. mori) has a significant impact on the economy by producing more than 80% of the globally produced raw silk. The exposure of silkworm to pesticides may cause adverse effects on B. mori, such as a reduction in the production and quality of silk. This study aims to assay the effect of venom from the jellyfish Nemopilema nomurai on growth, cuticle and acetylcholinesterase (AChE) activity of the silkworm B. mori by the leaf dipping method. The experimental results revealed that the four samples caused neither antifeeding nor a lethal effect on B. mori. The sample SFV inhibited B. mori growth after 6 days of treatment in a dose-dependent manner. The samples SFV, DSFV and Fr-1 inhibited the precipitation and synthesis of chitin in the cuticle after 12 and 14 days of treatment. In the case of the four samples, the AChE was significantly improved after 14 days of treatment.

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Section: Resultsmentioning

confidence: 99%

Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx mori L.

Chen

et al. 2015

Toxins

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“…The function of the encapsulation and phagocytosis processes of insect hemocytes were found to be destroyed by destruxins [22]. In our previously survey, it was also found that DA could induce obviously morphologic alterations of silkworm's hemocytes in vivo , even at an extremely low dose [23]. Furthermore, another study showed that destruxinss may play a key role in suppressing the innate immune response of Drosophila melanogaster , by inhibiting the expression of antimicrobial peptides [24].…”

Section: Introductionmentioning

confidence: 82%

Gene Expression Profile of Bombyx mori Hemocyte under the Stress of Destruxin A

et al. 2014

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Destruxin A (DA) is a cyclo-peptidic mycotoxin from the entomopathogenic fungus Metarhizium anisopliae. To uncover potential genes associated with its molecular mechanisms, a digital gene expression (DGE) profiling analysis was used to compare differentially expressed genes in the hemocytes of silkworm larvae treated with DA. Ten DGE libraries were constructed, sequenced, and assembled, and the unigenes with least 2.0-fold difference were further analyzed. The numbers of up-regulated genes were 10, 20, 18, 74 and 8, as well as the numbers of down-regulated genes were 0, 1, 8, 13 and 3 at 1, 4, 8, 12 and 24 h post treatment, respectively. Totally, the expression of 132 genes were significantly changed, among them, 1, 3 and 12 genes were continually up-regulated at 4, 3 and 2 different time points, respectively, while 1 gene was either up or down-regulated continually at 2 different time points. Furthermore, 68 genes were assigned to one or multiple gene ontology (GO) terms and 89 genes were assigned to specific Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology. In-depth analysis identified that these genes putatively involved in insecticide resistance, cell apoptosis, and innate immune defense. Finally, twenty differentially expressed genes were randomly chosen and validated by quantitative real-time PCR (qRT-PCR). Our studies provide insights into the toxic effect of this microbial insecticide on silkworm's hemocytes, and are helpful to better understanding of the molecular mechanisms of DA as a biological insecticide.

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“…For this, it is necessary to elucidate the targets of DA acting on insects. In the past decade, several studies indicated that DA changes the morphology of hemocytes and brings on equilibrium chaos of intra-and extra-cellular hydrogen and calcium ion in Bombyx mori [4,5], and regulates immune related gene expression [6,7]. Recently, a few DA binding-proteins in silkworm were found [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Interactions of Destruxin A with Silkworms’ Arginine tRNA Synthetase and Lamin-C Proteins

Wang¹,

Weng²,

Yin³

et al. 2020

Toxins

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Destruxin A (DA), a cyclodepsipeptidic mycotoxin produced by entomopathogenic fungus Metarhizium anisopliae, has good insecticidal activity and potential to be a new pesticide. However, the mechanism of action is still obscure. Our previous experiments showed that DA was involved in regulation of transcription and protein synthesis and suggested that silkworms’ arginine tRNA synthetase (BmArgRS), Lamin-C Proteins (BmLamin-C) and ATP-dependent RNA helicase PRP1 (BmPRP1) were candidates of DA-binding proteins. In this study, we employed bio-layer interferometry (BLI), circular dichroism (CD), cellular thermal shift assay (CETSA), and other technologies to verify the interaction of DA with above three proteins in vitro and in vivo. The results of BLI indicated that BmArgRS and BmLamin-C were binding-protein of DA with KD value 5.53 × 10−5 and 8.64 × 10−5 M, but not BmPRP1. These interactions were also verified by CD and CETSA tests. In addition, docking model and mutants assay in vitro showed that BmArgRS interacts with DA at the pocket including Lys228, His231, Asp434 and Gln437 in its enzyme active catalysis region, while BmLamin-C binds to DA at His524 and Lys528 in the tail domain. This study might provide new insight and evidence in illustrating molecular mechanism of DA in breaking insect.

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Effects of Destruxin A on Hemocytes Morphology of Bombyx mori

Cited by 29 publications

References 20 publications

Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx mori L.

Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx mori L.

Gene Expression Profile of Bombyx mori Hemocyte under the Stress of Destruxin A

Interactions of Destruxin A with Silkworms’ Arginine tRNA Synthetase and Lamin-C Proteins

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