Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen

Chanapiwat, Panida; Kaeoket, Kampon; Tummaruk, Padet

doi:10.1038/aja.2009.40

Cited by 53 publications

(66 citation statements)

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“…It is observed a higher percentage of functional membrane sperm and a lower percentage of damaged cells after thawing in the diluent where 2% of salmon oil was added as compared to the control group. This result corroborates the results found by Andrade (2013) in horses and Chanapiwat et al (2009), who studied the effect of DHA semen quality of swine semen, where they found a better result obtained by the combination of egg yolk enriched in DHA + L-cysteine. This study demonstrates the importance of the role of this association in the protection of the structure and function of the plasma membrane, increasing PUFA levels in the membrane, improving its fluidity and flexibility due to the presence of some double bonds.…”

Section: Resultssupporting

confidence: 82%

The addition of the salmon oil in the freezing of equine semen

Agostinho

Andrade

Caiado

et al. 2017

Rev. bras. saúde prod. anim.

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SUMARY The aim of the present study was to verify the effect of salmon oil addition on cryopreservation of equine semen. The experiment consisted of two treatments. Treatment 1 (T1) (control diluent), BotuCrio® was used without addition of salmon oil and treatment 2 (T2) (experimental diluent) BotuCrio® plus (with) 2% salmon oil. Three ejaculates of four stallions were used, totalizing 12 collections (n=12). Overall motility and progressive motility were evaluated by the Hamilton Thorn Research (HTR) Ceros 10.8 program, as well as the plasma membrane functionality through the hyposmotic test. Both treatments did not present statistical differences in relation to motility (T1 25,2±1,7 a, T2 29,7±1,9 a) and progressive motility (T1 11,0±1,1 a, T2 14,1±1,3 a). With respect to the hyposmotic test, the treatment 2 plus 2% of Salmon oil, presented better protection of sperm membrane functionality in relation to the control treatment (T2 77,3±1,0 a, T1 68,0±1,0 b). It can be concluded that salmon oil, although not altering the total and progressive motility, confers a better efficiency of sperm membrane functionality after thawing in equine semen.

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Section: Resultssupporting

confidence: 82%

The addition of the salmon oil in the freezing of equine semen

Agostinho

Andrade

Caiado

et al. 2017

Rev. bras. saúde prod. anim.

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“…Diluted semen was transferred to 50 mL centrifuge tubes, cooled at 15ºC for 120 min and later centrifuged at 800 × g at 15ºC for 10 min (Hettich Rotanta 460R; Andreas Hettich GmbH & Co. KG, Tuttlingen, Germany). The supernatant was discarded and the sperm pellet was re-suspended (about 1-2:1) with Extender II (80 mL of 11% lactose and 20 mL egg yolk) to a concentration of 1.5 × 10 9 spermatozoa per mL [8]. To .…”

Section: Semen Freezing Processmentioning

confidence: 99%

“…T h e i n t e g r i t y o f t h e s p e r m a c r o s o m e w a s evaluated using fluorescein isothiocyanate-labelled peanut (A. Hypogaea) agglutinin (FITC-PNA) staining [8,[24][25][26][27]. Ten µL of diluted semen with 140 µL phosphate buffered saline (PBS) was mixed with 10 µL of EthD-1 and incubated at 37ºC for 15 min.…”

Section: Assessment Of Acrosomal Integritymentioning

confidence: 99%

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Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

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Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L −1 (group I, control), 5 mmol L −1 (group II), 10 mmol L −1 (group III) and 15 mmol L −1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L −1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.

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“…Diluted semen was transferred to 50 ml centrifuge tubes, cooled at 15°C for 120 min and later centrifuged at 800 × g 15°C for 10 min (Hettich Rotanta 460R, Andreas Hettich GmbH & Co., KG, Tuttlingen, Germany). The supernatant was discarded, and the sperm pellet was re-suspended (about 1-2:1) with extender II (80 ml of 11% lactose and 20 ml egg yolk, LEY) to a concentration of 1.5 × 10 9 spermatozoa/ml [9,23,24,29]. To prepare LEY freezing Extender II supplemented with gamma-oryzanol, two types of gamma-oryzanol-enriched rice bran oil, i.e., from a dietary supplement product (Biogrow Natural TM , Biogrow (TH) Co., Ltd., Bangkok, Thailand) and from a salad dressing and cooking oil (King TM rice bran oil, Thai Edible Oil Co., Ltd., Bangkok, Thailand) were used and prepared as follows to provide a concentration of gammaoryzanol of 0.1 mg/ml of Extender II.…”

Section: Methodsmentioning

confidence: 99%

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

Kaeoket

Donto

Nualnoy

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of this study was to determine the effect of gamma-oryzanol-enriched -rice bran oil on the quality of cryopreserved boar semen. Ten boars provided semen of proven motility and morphology for this study. The semen was divided into three portions in which lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with 2 types of rice bran oils, at a gamma-oryzanol concentration of 0 mg/ml of lactose egg yolk (LEY) freezing extender (group A, control), 0.1 mg/ml(0.16 mMol) of freezing extender (group B) and 0.1 mg/ml of freezing extender (group C). Semen suspensions were loaded in medium straws (0.5 ml) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability and acrosomal integrity. There was a significantly higher percentage of progressive motility (34 versus 47.0 and 48.5, P<0.001), viability (35.5 versus 48.1 and 50.1, P<0.001) and acrosomal integrity (39.8 versus 50.8 and 54.9, P<0.001) in the gamma-oryzanolenriched rice bran oil-supplemented groups (groups B, C) than in the control group (group C), respectively. In conclusion, addition of gamma-oryzanol-enriched rice bran oil to LEY freezing extender is appropriated for improving the quality of frozen-thawed boar semen. Cryopreservation of boar spermatozoa offers an effective way to maintain their valuable genetic material and transport it between countries, thereby minimizing boar transportation. It has been demonstrated that certain processes in cryopreservation including elimination of seminal plasma by centrifugation and reduction in the temperature result in cold shock, owing to a decrease in quantity of antioxidants and an increase in reactive oxygen species (ROS) contents in the semen [6,8,43]. The ROS can lead to oxidative stress and lipid peroxidation of the sperm plasma membrane, which have detrimental effects on spermatozoa during cryopreservation, for instance, reduction in progressive motility, acrosomal integrity, sperm-oocyte fusion and fertilizing potential [1-3, 17, 41, 53].During the past decade, several studies have focused on supplementation of freezing extender for frozen boar semen with a variety of antioxidants (e.g., vitamin E, vitamin C, L-cysteine, glutathione, taurine, pyruvate, SOD, catalase) in an attempt to minimize the detrimental effects of ROS, which occur during the freezing process [7,10,16,23,26,38,39].Gamma-oryzanol, a phytosteryl ferulate mixture extracted from rice bran oil, has received a great deal of attention, because of its various significant health-promoting functions such as antioxidant activity, inhibition of lipoperoxidation by its scavenging activity [4], reduction in LDL cholesterol and induction of HDL cholesterol [40,41,48] and inhibition of platelet aggregation [13]. In humans, many studies have been reported on its potential implications as a UV-A filter in sunscreen cosmetics [14] and as a treatment for type 2 diabetes mellitus [12] and allergic reactions [3...

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Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen

Cited by 53 publications

References 58 publications

The addition of the salmon oil in the freezing of equine semen

The addition of the salmon oil in the freezing of equine semen

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

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