Effects of DHA supplementation of the extender containing egg yolk and α- tocopherol on the freezability and post-thawing fertility of ram semen

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

“…This is the first study illustrating that the addition of Vit E or Vit E + FO enriched serum directly to a commercial freezing medium could improve frozen‐thawed human sperm quality. In agreement with our results, Abdi‐Benemar et al () demonstrated that supplementating fish oil with α‐tocopherol in tris‐egg yolk extender increased motility, progressive motility, viability, membrane and acrosome integrity of frozen‐thawed ram spermatozoa. Moreover, higher sperm quality was reported in cryopreserved boar spermatozoa by the addition of fish oil in the freezing medium (Kaeoket et al, ).…”

“…Recently, the pivotal roles of omega–3 fatty acids, especially sperm docosahexaenoic acid (DHA) level on sperm function, were reported (Andersen et al, ). However, during the freeze–thaw procedure, the level of PUFA considerably decreases due to peroxidation of lipids (LPO) (Abdi‐Benemar et al, ). A high proportion of PUFA along with low level of cytoplasmic antioxidants make spermatozoa susceptible to ROS‐induced LPO (Topraggaleh et al, ), which significantly impairs sperm motility, viability and fertilising potential (Meseguer, Garrido, Simon, Pellicer, & Remohi, ).…”

“…To minimise the loss of membrane PUFAs during freeze–thaw procedure, dietary supplementation of PUFAs or direct addition of PUFAs enriched sources in cryopreservation medium has been reported in human and animals trials (Abavisani, Arshami, Naserian, Kandelousi, & Azizzadeh, ; Abdi‐Benemar et al, ; Esmaeili, Shahverdi, Alizadeh, Alipour, & Chehrazi, ; Habibi et al, ; Kaeoket, Sang‐urai, Thamniyom, Chanapiwat, & Techakumphu, ; Safarinejad, ; Towhidi, Zeinoaldini, Ardebili, Dadashpour Davachi, & Nasiri, ). Dietary supplementation with fish oil increased the level of the n–3 fatty acids in the serum (Nateghi, Alizadeh, Jafari Ahangari, Fathi, & Akhlaghi, ; Safarinejad, ) and sperm plasma membrane (Alizadeh, Esmaeili, Shahverdi, & Rashidi, ; Esmaeili et al, ; Safarinejad, ).…”

“…Moreover, improvement in frozen‐thawed sperm quality by dietary supplementation with PUFAs has been well established in previous studies (Esmaeili et al, ; Habibi et al, ). Sperm plasma membrane could utilise fatty acids from the PUFA‐enriched medium (Abdi‐Benemar et al, ). It has also been suggested that vitamin E might be tightly associated with the PUFAs in the diet, and a higher level of PUFA may affect the response to dietary fat/oil by antioxidant supplementation (Raederstorff, Wyss, Calder, Weber, & Eggersdorfer, ; Valk & Hornstra, ).…”

Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş-Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi

“…This situation is shown that the dose of 100 mg fish oil provides an ideal concentration in diluent so as to provide comfort to the spermatozoa, thus preventing damage to the process freezing, cryopreservation and thawing. These results are confirmed by previous reports by Abdi-Benemar et al [24] , who revealed that all frozen-thawed sperm characteristics are significantly enhanced with a surge in DHA levels from fish oil. One of the components of fish oil is PUFA with long chains and has been found in the spermatozoa of various species including ram, man and bull.…”