Effects of Diabetes on Calcium-Binding Protein in the Cecum and Colon of the Rat

Wilson, H.D.; Petith, M. Michael; Schedl, Harold P.

doi:10.1159/000198628

Cited by 5 publications

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Dietary Fructooligosaccharides Change the Concentration of Calbindin-D9k Differently in the Mucosa of the Small and Large Intestine of Rats

Ohta¹,

Motohashi

Ohtsuki³

et al. 1998

The Journal of Nutrition

View full text Add to dashboard Cite

Previously, we confirmed that dietary fructooligosaccharides (FOS) increase calcium absorption in rats. In this study, we examined the influence of FOS feeding on the concentration of calbindin-D9k of several intestinal segments in rats. Rats in the control group were fed a diet without FOS. Rats in the other two groups were fed the diet containing FOS at either 50 or 100 g/kg for 10 d and subjected to a calcium absorption study. On the final day of feeding, the rats were killed and the entire intestine was removed. The intestinal mucosa was collected from four segments, i.e., the proximal and distal segments of the small intestine, the cecum and the colorectum, respectively. The apparent absorption of calcium increased dose dependently (r = 0.9256, P < 0.0001). Significant positive correlations between apparent calcium absorption and the relative amounts of calbindin in both large intestinal segments were observed (cecum, r = 0.8956, P = 0.0011; colorectum, r = 0.8828, P = 0.0016). Also, significant negative correlations between apparent calcium absorption and the relative amounts of calbindin-D9k in both small intestinal segments were observed (proximal, r = -0.7149, P = 0. 0304; distal, r = -0.8740, P = 0.0021). In conclusion, FOS feeding increases levels of calbindin-D9k in the large intestine, but decreases those in the small intestine. Moreover, these results suggest that part of the stimulatory effect of fructooligosaccharides relates to the transcellular route of calcium absorption in the large intestine of rats.

show abstract