Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats

Pérez-Gallardo, Rocío V.; Noriega‐Cisneros, Ruth; Esquivel‐Gutiérrez, Edgar; Calderón-Cortés, Elizabeth; Cortés‐Rojo, Christian; Manzo‐Ávalos, Salvador; Campos-Garcı́a, Jesús; Salgado‐Garciglia, Rafael; Montoya‐Pérez, Rocío; Boldogh, István; Saavedra‐Molina, Alfredo

doi:10.1007/s10863-014-9594-4

Cited by 29 publications

(14 citation statements)

References 51 publications

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“…The decrease in antioxidant capacity and increase in oxidative stress product break down the dynamic balance between antioxidation and oxidation in vivo. 19,20 T-AOC is an important marker of oxidation, which mainly reflects nonenzymatic but includes the activity of a minority of small molecular enzymatic systems. MDA is a naturally occurring product of lipid peroxidation and prostaglandin biosynthesis that is mutagenic and carcinogenic.…”

Section: Discussionmentioning

confidence: 99%

The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats

et al. 2015

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Diabetic nephropathy (DN) is one of the most common chronic complications of diabetes, which is associated with an increased oxidative stress induced by hyperglycemia and alterations in DJ-1/NF-E2-related factor-2 (Nrf2) pathway. In the present study, we investigated the role and the proper time nodes of DJ-1/Nrf2 pathway in the pathogenesis of DN. Diabetes mellitus (DM) model of rats was induced by intraperitoneal injection of streptozotocin (STZ) on male Sprague-Dawley (SD) rats. Then, the diabetic rats were divided into 4, 8 and 12 weeks groups. As early at 4 weeks of diabetes, renal histologic evaluation score, cystatin C (Cys C), b2-microglobulin (b2-MG) and malondialdehyde (MDA) levels were increased, and total antioxidative capacity (T-AOC) level was decreased as compared with that in the control group. The protein expressions of DJ-1, NF-E2-related factor-2 (Nrf2) and heme oxygenase-1 (HO-1) were upregulated compared with the control group from 4 weeks and further increased with the progression of DM. The protein expressions of DJ-1, Nrf2 and HO-1 in renal tissues have good line correlations with renal histologic evaluation score, respectively. Taken together, these results suggested that the activation of DJ-1/Nrf2 pathway was involved in the pathogenesis of diabetic nephropathy in rats.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats

et al. 2015

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“…It has also been described that NO can reach local higher levels than the average concentration in tissues [29] and that association of iNOS with the mitochondrial outer membrane increases under pathological conditions, such as sepsis and inflammation [39], correlating well with the proposed role of CACT S-nitrosylation. Moreover increase of NO is reported in diabetics rats [40]. Indeed, reducing intramitochondrial acetyl-CoA accumulation may have a beneficial effect in type 2 diabetes [41].…”

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confidence: 96%

Nitric oxide inhibits the mitochondrial carnitine/acylcarnitine carrier through reversible S -nitrosylation of cysteine 136

Tonazzi

Giangregorio

Console

et al. 2017

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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S-nitrosylation of the mitochondrial carnitine/acylcarnitine transporter (CACT) has been investigated on the native and the recombinant proteins reconstituted in proteoliposomes, and on intact mitochondria. The widely-used NO-releasing compound, GSNO, strongly inhibited the antiport measured in proteoliposomes reconstituted with the native CACT from rat liver mitochondria or the recombinant rat CACT over-expressed in E. coli. Inhibition was reversed by the reducing agent dithioerythritol, indicating a reaction mechanism based on nitrosylation of Cys residues of the CACT. The half inhibition constant (IC50) was very similar for the native and recombinant proteins, i.e., 74 and 71μM, respectively. The inhibition resulted to be competitive with respect the substrate, carnitine. NO competed also with NEM, correlating well with previous data showing interference of NEM with the substrate transport path. Using a site-directed mutagenesis approach on Cys residues of the recombinant CACT, the target of NO was identified. C136 plays a major role in the reaction mechanism. The occurrence of S-nitrosylation was demonstrated in intact mitochondria after treatment with GSNO, immunoprecipitation and immunostaining of CACT with a specific anti NO-Cys antibody. In parallel samples, transport activity of CACT measured in intact mitochondria, was strongly inhibited after GSNO treatment. The possible physiological and pathological implications of the post-translational modification of CACT are discussed.

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“…DM is characterized by hyperglycemia, glucose intolerance, abnormal lipid and protein metabolisms along with specific long–term complications affecting several major organs, including heart, blood vessels, nerves, eyes, and kidneys. Diabetic complications, such as cardiac dysfunction, atherosclerosis, neuropathy, retinopathy, and nephropathy23 are linked to defective insulin secretion, insulin resistance, or both45. Patients with type 2 diabetes (T2D) are at higher risk of complications arising from macro vascular, peripheral vascular and coronary heart disease, stroke, and insulin resistance associated with atherosclerosis6.…”

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confidence: 99%

Purslane (Portulaca oleracea) Seed Consumption And Aerobic Training Improves Biomarkers Associated with Atherosclerosis in Women with Type 2 Diabetes (T2D)

Dehghan

Soori

Gholami

et al. 2016

Sci Rep

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The aim of this study was to investigate the responses of atherosclerosis plaque biomarkers to purslane seed consumption and aerobic training in women with T2D. 196 women with T2D were assigned into; (1) placebo (PL), (2) aerobic training+placebo (AT + PL), 3) purslane seeds (PS), aerobic training+purslane seeds (AT + PS). The training program and purslane seeds consumption (2.5 g lunch and 5 g dinner) were carried out for 16 weeks. The components of purslane seed were identified and quantified by GC–MS. Blood samples were withdrawn via venipuncture to examine blood glucose, low-density lipoprotein (LDL), high-density lipoprotein (HDL), cholesterol, triglycerides (TG), creatinine, urea, uric acid, NF-κB, GLP1, GLP1R, TIMP-1, MMP2, MMP9, CRP, CST3, and CTSS expressions. Blood glucose, LDL, cholesterol, TG, creatinine, urea, and uric acid levels in the (P), (AT), and (AT + PS) groups were significantly decreased compared to the pre-experimental levels or the placebo group, while HDL, significantly increased. Furthermore, the protein and mRNA levels of NF-κB, TIMP-1, MMP2 &9, CRP, CST3, and CTSS in the (P), (AT), (AT + PS) significantly decreased compared to pre-experimental or the placebo group, while level of GLP1 and GLP1-R increased drastically. Findings suggest that purslane seed consumption alongside exercising could improve atherosclerosis plaque biomarkers through synergistically mechanisms in T2D.

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Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats

Cited by 29 publications

References 51 publications

The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats

The role of DJ-1/Nrf2 pathway in the pathogenesis of diabetic nephropathy in rats

Nitric oxide inhibits the mitochondrial carnitine/acylcarnitine carrier through reversible S -nitrosylation of cysteine 136

Purslane (Portulaca oleracea) Seed Consumption And Aerobic Training Improves Biomarkers Associated with Atherosclerosis in Women with Type 2 Diabetes (T2D)

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