Effects of dialyzable leukocyte extracts(DLE) and inosine involved in DLE on lymphocytes.

文夫, 小松; 滋, 笹川

doi:10.3925/jjtc1958.34.530

Cited by 2 publications

(1 citation statement)

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“…This increase in inosine level may participate in the activation of lymphocytes that is induced by HD. Indeed, inosine contained in dialyzable leukocyte extracts activates lymphocytes (32).…”

Section: Abnormal Levels Of Ado An Its Metabolites and Enzymes In Patients Who Were Undergoing Hdmentioning

confidence: 99%

High Adenosine and Deoxyadenosine Concentrations in Mononuclear Cells of Hemodialyzed Patients

Sampol¹,

Dussol²,

Fenouillet³

et al. 2001

Journal of the American Society of Nephrology

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Abstract. Infections are one of the most important complications of hemodialysis (HD). The high concentrations of adenosine (Ado) and of its metabolites during HD may contribute to the dialysis-induced immune deficiency through their known ability to alter lymphocyte function. The influence of HD on Ado metabolism was assessed in mononuclear cells through the measurement of (1) the concentrations of nucleosides in mononuclear cells and (2) the activities of mononuclear cell Ado deaminase (MCADA) and Ado kinase, two enzymes involved in Ado concentration regulation. Nine end-stage renal failure hemodialyzed patients (five men and four women; mean age, 69 ± 10 yr) and eight healthy volunteers (four men and four women; mean age, 53 ± 19 yr) were included in the study. Before HD, Ado, deoxyadenosine, and inosine concentrations were respectively 2.9-, 2.5-, and 2.5-fold higher in mononuclear cells of patients than in healthy volunteers. During HD, Ado concentration decreased by 34%, whereas inosine concentration increased by 27%. Before HD, MCADA activity level was 2.1-fold lower in patients than in control subjects. After HD, MCADA activity increased by nearly 50% but remained lower than in control subjects. Ado kinase activity level of patients did not differ from that of control subjects and was unchanged by HD. The influence of Ado on in vitro mononuclear cell proliferation and interferon-γ production also was evaluated. Ado inhibited cell proliferation and interferon-γ production in a dose-dependent manner, and these inhibitions were stronger for patients than for healthy volunteers. The high concentrations of Ado and deoxyadenosine in mononuclear cells and the low MCADA activity level likely are involved in the immune defect of patients who are undergoing HD.

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Section: Abnormal Levels Of Ado An Its Metabolites and Enzymes In Patients Who Were Undergoing Hdmentioning

confidence: 99%