Effects of diamines on ornithine decarboxylase activity in control and virally transformed mouse fibroblasts

Bethell, Delia R.; Pegg, Anthony E.

doi:10.1042/bj1800087

Cited by 54 publications

(28 citation statements)

References 41 publications

(62 reference statements)

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“…In the colon, a precocious increase in activity at day 10 was only noted when rats were exposed to spermidine for 3 days prior to sacrifice. The increased levels of intestinal ODC ac tivity noted here after spermidine exposure to suckling rats are similar to those observed in the ileum of the adult rat after infusion of putrescine [42], Taken together, these results appear paradoxical in the context of the usual feedback inhibition of ODC by polyamines seen in a variety of cell types [9,10], However, a diminished sensitivitiy of ODC to autoregu lation by polyamines has been observed in virally transformed cells and in hepatocytes after carcinogen exposure [43][44][45]. Thus, a diminished responsiveness of ODC to poly amine levels may be characteristic of aug mented cell proliferation in a variety of systems.…”

Section: Discussionmentioning

confidence: 84%

Effect of Exogenously Administered Polyamine on the Structural Maturation and Enzyme Ontogeny of the Postnatal Rat Intestine

et al. 1993

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The factors regulating the developmental changes in intestinal morphology and enzyme activity during the postnatal period are incompletely understood. Increased ornithine decarboxylase (ODC) and polyamine levels occur in association with increased mucosal growth seen just prior to weaning. The present work examines the effects of the polyamine spermidine, administered exogenously during early postnatal development in the rat, on structural and functional differentiation of the intestine. Young rats were fed 6 μmol of spermidine for either 1 day (P1) or 3 days (P3) prior to sacrifice on postnatal day 10. Control littermates were sacrificed at day 10 (C10) or at day 49 (C49) (postweanling [adult] reference). A loss of most of the well-developed characteristic endosomal complex and supranuclear giant lysosome was observed in the absorptive cells of the ileum and proximal colon in the spermidine-treated groups and was accompanied by a decline in N-acetyl-glucosaminidase activity to adult levels. A precocious appearance of sucrase and NaK ATPase activities was observed in the P1 group and these activities attained adult levels in the P3 group. This premature appearance of sucrase and NaK ATPase activities was associated with a decline in lactase levels. The exogenous administration of spermidine also elicited an increase in mucosal ODC activity.

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Section: Discussionmentioning

confidence: 84%

Effect of Exogenously Administered Polyamine on the Structural Maturation and Enzyme Ontogeny of the Postnatal Rat Intestine

et al. 1993

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“…However, it has recently been shown (5-7, 13, 16) that in many dividing plant tissues putrescine is synthesized from ornithine by ODC, as found in microorganisms (1,22,30) and animal cells (4,11,23). After synthesis, putrescine may be converted into the other two major polyamines, spermidine and spermine.…”

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confidence: 99%

Polyamine Biosynthetic Enzymes in the Cell Cycle of Chlorella

Cohen¹,

Arad²,

Heimer³

et al. 1984

Plant Physiol.

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During the life cycle of Chlorella vulgaris Beijerinck var vulgaris fa. vulgaris growing synchronously, the specific activity of ornithine decarboxylase peaked at the 2nd hour of the cycle, whereas that of arginine decarboxylase changed only slightly, increasing towards the end of the cycle. The endogenous level of putrescine and spermidine on a per cell basis increased gradually up to the 8th hour of the cycle, and declined thereafter. Thus, the peak of ornithine decarboxylase activity and the polyamine increase preceded both DNA replication (which took place between the 6th and 8th hours of the cycle) and autospore release (which started at the 8th hour). A 2-fold increase in the light intensity caused doubling of the DNA content, resulting in doubling of the number of autospores per mother cell. It also brought about a 2-fold increase in the specific activity of ornithine decarboxylase and polyamine content, the peaks being at the same hour of the cycle under high and low light intensities. The increase in cell number and polyamine content in a Chlorella culture grown under high light intensity was inhibited by adifluoromethyl ornithine, a specific inhibitor of ornithine decarboxylase, this inhibition being partially reversed by putrescine.It is suggested that in C. vulgaris the sequence of events which relates polyamine biosynthesis to cell division is as follows: increased ornithine decarboxylase activity, accumulation of polyamines, DNA replication, and autospore release.

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“…However, its activity did not change during the logarithmic phase of growth, being one-fourth of this maximal level of ODC (E. Cohen, S. (Malis) Arad, Y. M. Heimer, and Y. Mizrahi, unpublished results). It was, therefore, suggested that in these two plant systems (8,9), as in mammalian cells (2,6,17), ODC is the first enzyme in polyamine biosynthetic pathway.…”

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confidence: 99%

Participation of Ornithine Decarboxylase in Early Stages of Tomato Fruit Development

Cohen¹,

Arad²,

Heimer³

et al. 1982

Plant Physiol.

128

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The apparent association of ornithine decarboxylase (ODC) with rapid cell proliferation in developing tomato (Lycopersicon esculentum Mil. cv.Pearson ms-35) fruits has been previously described. Further evidence is provided by the use of two ODC inhibitors, a-difluoromethylornithine (a-DFMO) and a-methylornithine (a-MO). Fruit development was inhibited by these inhibitors if applied during the period of intensive cell division. When applied in vitro, the two inhibitors were shown to inhibit the activity of ODC but not that of arginine decarboxylase (ADC). When applied in vivo, a-DFMO, a catalytic irreversible inhibitor, caused 97.1% reduction of ODC activity in the dialyzed extract from the treated ovaries, while it had no effect on ADC. On the other hand, a-MO, a reversible inhibitor, did not reduce the activity of these two enzymes in the dialyzed extracts when applied in vivo. The dialysis procedure probably removed a-MO from the enzyme fraction. Putrescine, the product of both ODC and ADC, aleviated the inhibition of fruit development but did not restore ODC activity to the control level. These results suggest that in the young developing tomato fruit, ODC is the enzyme responsible for the synthesis of putrescine, which is essential for the early stages of fruit development. The reduced activity of ODC elicited by putrescine suggests a mechanism of feedback regulation by enzyme repression or release of an ODC anti-enzyme.Polyamines are widely distributed in nature, but their precise role in cellular processes is not always fully understood. They are associated with cell proliferation, tissue regeneration, and malignancy (1,6,7,17,18). Most of the information on the biosynthetic pathways of putrescine, spermidine, and spermine, their regulation, and the possible site of action has been obtained from studies with microorganisms and mammalian cells (1). Such information is lacking for plant systems. Several reports (4,8,14,15,(20)(21)(22) do, however, describe the presence of various polyamines in plants and the occurrence of enzymes involved in polyamine biosynthesis, e.g. ODC3 and ADC. It is commonly accepted that ADC is the enzyme responsible for the production of putrescine in plants and that ODC is of lesser importance (14,15,(20)(21)(22)).We have recently described an apparent association between elevated ODC activity and rapid cell proliferation in two plant systems: tomato ovaries during the first 10 d after pollination and ' The work was performed in partial fulfillment of the requirements for the PhD thesis of E. C.'To whom correspondence should be addressed. 'Abbreviations: ODC, ornithine decarboxylase; ADC, arginine decarboxylase; a-DFMO, a-difluoromethylornithine; a-MO, a-methylornithine.tobacco XD cells growing in suspension culture during the logarithmic phase of growth (9). ADC was also present in tomato ovaries. However, its activity did not change during the logarithmic phase of growth, being one-fourth of this maximal level of ODC (E. Cohen, S. (Malis) Arad, Y. M. Heimer, and Y. Mizra...

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Effects of diamines on ornithine decarboxylase activity in control and virally transformed mouse fibroblasts

Cited by 54 publications

References 41 publications

Effect of Exogenously Administered Polyamine on the Structural Maturation and Enzyme Ontogeny of the Postnatal Rat Intestine

Effect of Exogenously Administered Polyamine on the Structural Maturation and Enzyme Ontogeny of the Postnatal Rat Intestine

Polyamine Biosynthetic Enzymes in the Cell Cycle of Chlorella

Participation of Ornithine Decarboxylase in Early Stages of Tomato Fruit Development

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