Effects of diaspirin crosslinked hemoglobin infusion in treadmill-exercised swine

Crago, Mark S.; West, Shanda; McKenzie, Jack E.

doi:10.1007/bf02481737

Cited by 7 publications

(4 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In animals, Crago et al [3] studied five healthy, nonanaemic swine infused with 5 ml/kg of a 10% diaspirin crosslinked haemoglobin solution (DCLHb) during graded treadmill exercise, and reported a higher oxygen extraction in the DCLHb group accompanied by a decrease in cardiac output. This effect was evident during both exercise and the recovery periods, and was interpreted to be indicative of an enhanced capacity for oxygen delivery mediated by the HBOC solution.…”

Section: Discussionmentioning

confidence: 99%

Effects of Hemopure™ on Maximal Oxygen Uptake and Endurance Performance in Healthy Humans

Ashenden¹,

Schumacher²,

Sharpe³

et al. 2007

Int J Sports Med

View full text Add to dashboard Cite

Haemoglobin-based oxygen carriers (HBOCs) such as Hemopure are touted as a tenable substitute for red blood cells and therefore potential doping agents, although the mechanisms of oxygen transport of HBOCs are incompletely understood. We investigated whether infusion of Hemopure increased maximal oxygen uptake (V.O 2max) and endurance performance in healthy subjects. Twelve male subjects performed two 4-minute submaximal exercise bouts equivalent to 60 % and 75 % of V.O (2max) on a cycle ergometer, followed by a ramped incremental protocol to elicit V.O (2max). A crossover design tested the effect of infusing either 30 g (6 subjects) or 45 g (6 subjects) of Hemopure versus a placebo. Under our study conditions, Hemopure did not increase V.O (2max) nor endurance performance. However, the infusion of Hemopure caused a decrease in heart rate of approximately 10 bpm (p=0.009) and an average increase in mean ( approximately 7 mmHg) and diastolic blood pressure ( approximately 8 mmHg) (p=0.046) at submaximal and maximal exercise intensities. Infusion of Hemopure did not bestow the same physiological advantages generally associated with infusion of red blood cells. It is conceivable that under exercise conditions, the hypertensive effects of Hemopure counter the performance-enhancing effect of improved blood oxygen carrying capacity.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Hemopure™ on Maximal Oxygen Uptake and Endurance Performance in Healthy Humans

Ashenden¹,

Schumacher²,

Sharpe³

et al. 2007

Int J Sports Med

View full text Add to dashboard Cite

show abstract

“…Contrasting this, Crago et al [9] showed in five healthy, non blood-depleted swine that after infusion of 5 ml/kg of 10 % DCLHb-solution, oxygen delivery and recovery was improved during graded treadmill exercise. Oxygen debt was reduced due to higher oxygen delivery to the tissue.…”

Section: Oxygen Delivery To the Muscle And Implications For Sports Mementioning

confidence: 90%

Artificial Oxygen Carriers - The New Doping Threat in Endurance Sport?

Schumacher

Schmid²,

Dinkelmann³

et al. 2001

Int J Sports Med

View full text Add to dashboard Cite

Maximal oxygen uptake is the major performance limiting factor in endurance sports. Sophisticated training methods have been developed to increase this variable. On the other hand, attempts have been made to improve maximal oxygen uptake by artificial means: blood doping and the misuse of recombinant human erythropoietin have beneficial effects on aerobic exercise capacity. Both methods have been banned by international sporting federations. A new class of substances might represent the next step of fraudulent improvement of the maximal oxygen uptake: artificial oxygen carriers, such as solutions based on recombinant, bovine or human hemoglobin and perfluorocarbon-emulsions have been shown to improve oxygen delivery to the muscle. Hemoglobin-based solutions improve aerobic exercise capacity in animal and human testing. Both substances have potentially lethal side effects including renal toxicity, increased systemic and pulmonary blood pressure and impairment of the immune system. Hemoglobin-based carriers can be detected in drug testing with routine laboratory tests based on the detection of free hemoglobin. Perfluorocarbon is not metabolized by the body and exhaled through the lung and can be measured with chromatography. No screening for these substances in drug tests has been performed so far. International sporting federations should be aware of this new, emerging doping threat.

show abstract

“…In addition to the restoration of intravascular volume and oxygen, HBOC seem to have a higher oxygenation potency when compared to RBCs [75] because of their plasmatic oxygen transport and enhanced oxygen release from the HBOC themselves and from adjacent erythrocytes [76]. The increased oxygen extraction seems to be a characteristic essential of modern cell-free hemoglobin and has been proved in different animal models [75,77]. There is evidence from animal studies that the vasoconstrictive side effects associated with HBOC treatment, whether they are caused by NO scavenging [78], endothelin release [79] or modulation of alpha-adrenoreceptors [80], are more than compensated by the enhanced oxygen off-load through capillaries.…”

Section: Hemoglobin Solutionsmentioning

confidence: 99%

Artificial Oxygen Carriers as Red Blood Cell Substitutes – Perfluorocarbons and Cell-Free Hemoglobin

Standl¹

2000

Transfus Med Hemother

View full text Add to dashboard Cite

Forthcoming shortfall of blood products and persisting concerns about viral transmission and immunosuppressive side effects of allogeneic blood transfusion have reinforced the studies with alternative oxygen carriers in the last years. Modern perfluorochemicals and cell-free hemoglobin solutions can be applied without prior cross-matching and are now available as stable formulations with long shelf life. Both groups of oxygen carriers have shown their effectivity and tolerability in numerous animal studies. An emulsion of perflubron which has a 60% weight/volume relation is actually undergoing phase III studies with respect to its effectivity in augmented acute normovolemic hemodilution since it has been shown to reverse hemodynamic transfusion triggers. While clinical studies with human cross-linked hemoglobin (DCLHb) have been stopped last year because of the results of two clinical trials showing an increased mortality in patients with stroke and multiple injury shock being treated with DCLHb in comparison with saline, a phase III study with polymerized bovine hemoglobin HBOC-201 is actually being performed in noncardiac patients with perioperative bleeding. The objective of this multicenter study is to show that treatment with HBOC-201 can reduce or avoid allogeneic RBC transfusion. Besides its use in clinical transfusion protocols, artificial oxygen carriers have a unique potential to deliver oxygen to the tissues by plasmatic transport due to its different physiology of oxygenation when compared with conventional oxygenation provided by red blood cells. Future studies must show if these modern oxygen carriers are able to improve outcome of patients with impaired perfusion and organ oxygenation.

show abstract

Effects of diaspirin crosslinked hemoglobin infusion in treadmill-exercised swine

Cited by 7 publications

References 25 publications

Effects of Hemopure™ on Maximal Oxygen Uptake and Endurance Performance in Healthy Humans

Effects of Hemopure™ on Maximal Oxygen Uptake and Endurance Performance in Healthy Humans

Artificial Oxygen Carriers - The New Doping Threat in Endurance Sport?

Artificial Oxygen Carriers as Red Blood Cell Substitutes – Perfluorocarbons and Cell-Free Hemoglobin

Contact Info

Product

Resources

About